Photosynthetica 2002, 40(4):523-527 | DOI: 10.1023/A:1024339716382

Protective Effects of Exogenous Antioxidants and Phenolic Compounds on Photosynthesis of Wheat Leaves under High Irradiance and Oxidative Stress

Hui Jie Zhao1, Qi Zou2
1 Department of Agronomy, Henan Agricultural University, Zhengzhou, Henan, P.R. China
2 Department of Plant Science, Shandong Agricultural University, Tai-An, Shandong, P.R. China

Infiltration of methyl viologen (MV, source of O2-) and Na-diethyldithiocarbamate (DDC, inhibitor of SOD) into wheat leaves resulted in the accumulation of active oxygen species and photo-oxidative damage to photosynthetic apparatus under both moderate and high irradiance. Exogenous antioxidants, ascorbate (ASA) and mannitol, scavenged active oxygen efficiently, protected the photosynthetic system from MV and DDC induced oxidative damage, and maintained high Fv/Fm [maximal photochemical efficiency of photosystem 2 (PS2) while all PS2 reaction centres are open], Fm/F0 (another expression for the maximal photochemical efficiency of PS2), ΦPS2 (actual quantum yield of PS2 under actinic irradiation), qP (photochemical quenching coefficient), PN (net photosynthetic rate), and lowered qNP (non-photochemical quenching coefficient) of the leaves kept under high irradiance and oxidative stress. Phenolic compounds used in these experiments, catechol (Cat), resorcinol (Res), and tannic acid (Tan), had similar anti-oxidative activity and protective effect on photosynthetic apparatus as ASA and mannitol. The anti-oxidative activity and the protective effect of phenolic compounds increased with increase in their concentration from 100 to 300 g m-3. The number and the position of hydroxyl group in phenolic molecules seemed to influence their antioxidative activity.

Additional key words: ascorbate; catechol; Na-diethyldithiocarbamate; irradiance; mannitol; photosystem 2; resorcinol; tannic acid; Triticum

Published: December 1, 2002  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Zhao, H.J., & Zou, Q. (2002). Protective Effects of Exogenous Antioxidants and Phenolic Compounds on Photosynthesis of Wheat Leaves under High Irradiance and Oxidative Stress. Photosynthetica, 40(4), 523-527. doi: 10.1023/A:1024339716382
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Allen, R.D.: Dissection of oxidative stress tolerance using transgenic plants.-Plant Physiol. 107: 1049-1054, 1995. Go to original source...
    2. Asada, K.: Radical production and scavenging in the chloroplasts.-In: Baker, N.R. (ed.): Photosynthesis and the Environment. Pp. 123-150. Kluwer Academic Publishers, Dordrecht-Boston-London 1996. Go to original source...
    3. Asada, K., Kiso, K., Yoshikawa, K.: Univalent reduction of molecular oxygen by spinach chloroplasts on illumination.-J. biol. Chem. 249: 2175-2181, 1974. Go to original source...
    4. Bowler, C., Montagu, W.V.: Superoxide dismutase and stress tolerance.-Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 83-116, 1992. Go to original source...
    5. Demmig-Adams, B., Adams, W.W., III: Photoprotection and other responses of plants to high light stress.-Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 599-626, 1992. Go to original source...
    6. Elstner, E.F., Heupel, A.: Inhibition of nitrite formation from hydroxylammonium chloride: A simple assay for superoxide dismutase.-Anal. Biochem. 70: 616, 1976. Go to original source...
    7. Foyer, C.H., Descourvières, P., Kunert, K.J.: Protection against oxygen radicals: an important defence mechanism studied in transgenic plants.-Plant Cell Environ. 17: 507-523, 1994. Go to original source...
    8. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    9. Inzé, D., Montague, M.V.: Oxidative stress in plants.-Cur. Opinions Biotechnol. 6: 153-158, 1995. Go to original source...
    10. Larson, R.A.: The antioxidants of higher plants.-Phytochemistry 27: 969-978, 1988. Go to original source...
    11. McCord, J.M., Fridovich, I.: Superoxide dismutase: An enzymic function for erythrocuprin (hemocuprin).-J. biol. Chem. 224: 6049-6055, 1969. Go to original source...
    12. Monk, L.S., Fagerstedt, K.V., Crawford, R.M.M.: Oxygen toxicity and superoxide dismutase as an antioxidatant in physiological stress.-Physiol. Plant. 76: 456-459, 1989. Go to original source...
    13. Osmond, C.B.: What is photoinhibition? Some insights from comparisons of shade and sun plants.-In: Baker, N.P., Bowyer, J.R. (ed.): Photoinhibition of Photosynthesis: From Molecular Mechanisms to the Environment. Pp. 1-24. Bios Scientific Publishers, Oxford 1994.
    14. Powles, S.B.: Photoinhibition of photosynthesis induced by visible light.-Annu. Rev. Plant Physiol. 35: 15-44, 1984. Go to original source...
    15. Richter, M., Rühle, W., Wild, A.: Studies on the mechanism of photosystem II photoinhibition. II. The involvement of toxic oxygen species.-Photosynth. Res. 24: 237-243, 1990. Go to original source...
    16. Salah, N., Miller, N.J., Paganga, G., Tijburg, L., Bolwell, G.P., Rice-Evans, C.: Polyphenolic flavanols as scavengers of aqueous phase radicals and chain-breaking antioxidants.-Arch. Biochem. Biophys. 322: 339-346, 1995. Go to original source...
    17. Schreiber, U., Schliwa, U., Bilger, W.: Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer.-Photosynth. Res. 10: 51-62, 1986. Go to original source...
    18. Ye, L., Gao, H., Zou, Q.: Responses of the antioxidant systems and xanthophyll cycle in Phaseolus vulgaris to the combined stress of high irradiance and high temperature.-Photosynthetica 38: 205-210, 2000. Go to original source...
    19. Yordanov, I., Velikova, V., Tsonev, T.: Plant responses to drought, acclimation, and stress tolerance.-Photosynthetica 38: 171-186, 2000. Go to original source...
    20. Zhou, B., Jia, Z.S., Chen, Z.H., Yang, L., Wu, L.M., Liu, Z.L.: Synergistic antioxidant effect of green tea polyphenols with α-tocopherol on free radical initiated peroxidation of linoleic acid in micelles.-J. chem. Soc. Perkin Trans. 2 2000: 785-791, 2000. Go to original source...

    Return to the content