Abstract
Four strains of an unknown yeast species were isolated from rotten willow samples which were collected in Hungary. Although their phenotypic characteristics suggested that they were conspecific with Pichia pastoris, investigation of their small (18S) and large (26S) subunit rDNA revealed that they belonged to an undescribed yeast species. The description of the new yeast species, Pichia (Komagataella) pseudopastoris is given.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W. et al. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389–3402.
Barnett J.A., Payne R.W. and Yarrow D. 2000. Yeasts: Characteristics and identification. 3rd edn. Cambridge Univ. Press, Cambridge.
Dlauchy D., Tornai-Lehoczki J. and Péter G. 1999. Restriction enzyme analysis of PCR amplified rDNA as a taxonomic tool in yeast identification. Syst Appl Microbiol 22: 445–453.
Kurtzman C.P. 1998. Pichia E.C. Hansen emend Kurtzman. In: Kurtzman C.P. and Fell J.W. (eds), The Yeasts: A Taxonomic Study. 4th edn. Elsevier Science Publ., Amsterdam, pp. 273–352.
Kurtzman C.P. and Robnett C.J. 1998. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73: 331–371.
Kurtzman C.P. 2000. Three new ascomycetous yeasts from insect-associated arboreal habitats. Can J Microbiol 46: 50–58.
Phaff H.J. and Starmer W.T. 1987. Yeasts associated with plants, insects and soil. In: Rose A.H. and Harrison J.S. (eds), The Yeasts Vol. 1. 2nd edn. Academic Press, London, pp. 123–180.
Spencer J.F.T. and Spencer D.M. 1997. Ecology: where yeasts live. In: Spencer J.F.T. and Spencer D.M. (eds), Yeasts in Natural and Artificial Habitats. Springer, Berlin, pp. 33–58.
Vilgalys R. and Hester M. 1990. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bact 172: 4238–4246.
Wickerham L.J. and Burton K.A. 1954. A simple technique for obtaining mating types in heterothallic diploid yeasts, with special reference to their uses in the genus Hansenula. J Bact 67: 303–308.
White T.J., Bruns T., Lee S. and Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis N., Gelgand D., Sninsky J. and White T. (eds), PCR protocols: A Guide to Methods and Applications. Academic Press, Inc., New York, pp. 315–322.
Yamada Y. 1998. Identification of coenzyme Q (ubiquinone) homologs. In: Kurtzman C.P. and Fell J.W. (eds), The Yeasts: A Taxonomic Study. 4th edn. Elsevier Science Publ., Amsterdam, pp. 101–102.
Yamada Y., Matsuda M., Maeda K. and Mikata K. 1995. The phylogenetic relationships of methanol-assimilating yeasts based on the partial sequences of 18S and 26S ribosomal RNAs: The proposal of Komagataella gen. nov. (Saccharomycetaceae) Biosci. Biotech Biochem 59: 439–444.
Yarrow D. 1998. Methods for the isolation, maintenance and identification of yeasts. In: Kurtzman C.P. and Fell J.W. (eds), TheYeasts: A Taxonomic Study. 4th edn. Elsevier Science Publ., Amsterdam, pp. 77–100.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Dlauchy, D., Tornai-Lehoczki, J., Fülöp, L. et al. Pichia (Komagataella) pseudopastoris sp. nov., a new yeast species from Hungary. Antonie Van Leeuwenhoek 83, 327–332 (2003). https://doi.org/10.1023/A:1023318829389
Issue Date:
DOI: https://doi.org/10.1023/A:1023318829389