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Practical Use of Corrected Fluorescence Excitation and Emission Spectra of Fluorescent Proteins in Förster Resonance Energy Transfer (FRET) Studies

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Abstract

Corrected fluorescence excitation and emission spectra have been obtained from several enhanced variants of the green fluorescent protein (EGFP) isolated from the jellyfish Aequorea victoria, blue fluorescence protein (EBFP), cyan fluorescent protein (ECFP), EGFP and yellow fluorescent protein (EYFP–citrine) and from the red fluorescent protein (DsRed) isolated from the coral species Discosoma. The spectra are stored in a database. This report describes how the spectra can be used as templates to derive the critical transfer distance for any pair of fluorescent proteins.

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Authors and Affiliations

  1. MicroSpectroscopy Centre, Wageningen University, The Netherlands

    Mark A. Hink, Nina V. Visser, Jan Willem Borst, Arie van Hoek & Antonie J. W. G. Visser

  2. Laboratories of Biochemistry, Wageningen University, The Netherlands

    Mark A. Hink, Nina V. Visser, Jan Willem Borst & Antonie J. W. G. Visser

  3. Biophysics, Wageningen University, Dreijenlaan, 3 6703 HA, Wageningen, The Netherlands

    Arie van Hoek

  4. Department of Structural Biology, Faculty of Earth and Life Sciences, Vrije Universiteit, Amsterdam, The Netherlands

    Antonie J. W. G. Visser

Authors
  1. Mark A. Hink
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  2. Nina V. Visser
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  3. Jan Willem Borst
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  4. Arie van Hoek
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  5. Antonie J. W. G. Visser
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Correspondence to Antonie J. W. G. Visser.

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Hink, M.A., Visser, N.V., Borst, J.W. et al. Practical Use of Corrected Fluorescence Excitation and Emission Spectra of Fluorescent Proteins in Förster Resonance Energy Transfer (FRET) Studies. Journal of Fluorescence 13, 185–188 (2003). https://doi.org/10.1023/A:1022947411788

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  • DOI: https://doi.org/10.1023/A:1022947411788

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