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Larval Polytene Chromosomes of Black Flies (Simulium) from Thailand

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Abstract

Larval polytene chromosome maps of Simulium (G.) asakoae and S. (G.) sp. g in the ceylonicum-group and S. (G.) angulistylum, S. (G.) decuplum and S. (G.) siamense in the batoense-group of the subgenus Gomphostilbia from Thailand are presented. These species have three pairs of chromosomes (2n = 6). Light stained centromeric bands were observed in the chromosomes of S. (G.) asakoae, S. (G.) sp. g, S. (G.) decuplum and S. (G.) siamense, whereas heavy dark centromeric bands were present in S. (G.) angulistylum. The best distinguishing character of Simulium species in the subgenus Gomphostilbia is the position of the nucleolar organizer in the short arm of chromosome I. The Ring of Balbiani and the double bubble are located in chromosome arm IIS in all species except for S. (G.) angulistylum, which showed these cytological markers in chromosome arm IIIS. A low chromosomal polymorphism was recorded in all species except for S. (G.) sp. g, which exhibited a standard polytene chromosome. Inversion polymorphisms found in this study conformed to Hardy–Weinberg equilibrium and were not associated with sex. These species have different specific markers and banding patterns although homologous banding sequences were found in chromosome arm IIS in S. (G.) asakoae, S. (G.) sp. g, S. (G.) decuplum and S. (G.) siamense and chromosome arm IIIS in S. (G.) angulistylum. Our results showed no evidence of a sibling species complex within any taxon.

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References

  • Arteaga, L.T. & d.H.P. Munoz, 1999. New cytotype in the Simulium metallicum complex (Diptera: Simuliidae) from Cundinamarca, Colombia. J. Med. Ent. 36: 133–140.

    Google Scholar 

  • Bedo, D.G., 1977. Cytogenetics and evolution of Simulium ornatipes Skuse (Diptera: Simuliidae). I. Sibling speciation. Chromosoma 64: 37–65.

    Google Scholar 

  • Bedo, D.G., 1979. Cytogenetics and evolution of Simulium ornatipes Skuse (Diptera: Simuliidae). II. Temporal variation in chromosome polymorphisms and homosequential sibling species. Evolution 33: 296–308.

    Google Scholar 

  • Boakye, D.A., 1993. A pictoral guide to the chromosomal identification of members of the Simulium damnosum Theobald complex in West Africa with particular reference to the Onchocerciasis Control Programme Area. Trop. Med. Parasitol. 44: 223–240.

    Google Scholar 

  • Boakye, D.A. & F.W. Mosha, 1988. The distribution and chromosome polymorphism of Simulium dieguerense (Diptera: Simuliidae). Trop. Med. Parasitol. 39: 117–119.

    Google Scholar 

  • Campos, J., S.M. Reccopimentel & C.F.S. Andrade, 1996. Polytene chromosome analysis of a population of Simulium pertinax (Diptera, Simuliidae). Brazilian J. Genet. 19: 47–52.

    Google Scholar 

  • Charalambous, M., A.J. Shelley, M.M. Herzog & A.P. Dias, 1996. Four new cytotypes of the onchocerciasis vector blackfly Simulium guianense in Brazil. Med. Vet. Ent. 10: 111–120.

    Google Scholar 

  • Crosskey, R.W., 1988. Annotated checklist of the world black flies (Diptera: Simuliidae), pp. 425–520 in Black Flies: Ecology, Population Management, and Annotated World List, edited by K.C. Kim & R.W. Merritt. Pennsylvania State University Press, University Park.

    Google Scholar 

  • Datta, M., 1983. A review of the Simuliidae (Diptera) from the Oriental Region. Oriental Insects 17: 215–267.

    Google Scholar 

  • Dobzhansky, T., H. Burla & A.B. Da Cunha, 1950. A comparative study of chromosomal polymorphism in sibling species of the willistoni group of Drosophila. Am. Nat. 84: 229–246.

    Google Scholar 

  • Hadi, U.K., H. Takaoka & C. Aoki, 1995. Larval salivary gland chromosomes of the blackfly, Simulium (Gomphostilbia) yaeyamaense (Diptera: Simuliidae) from Ryukyu Islands, Japan. Jpn. J. Sanit. Zool. 46: 235–239.

    Google Scholar 

  • Hadi, U.K., H. Takaoka, K. Kondo & H. Hirai, 1996a. Larval salivary gland chromosomes of the blackfly, Simulium (Gomphostilbia) sundaicum (Diptera: Simuliidae) from Java, Indonesia. Med. Entomol. Zool. 47: 55–61.

    Google Scholar 

  • Hadi, U.K., H. Takaoka, K. Kondo & H. Hirai, 1996b. Larval salivary gland chromosomes of Simulium (Simulium) eximium (Diptera: Simuliidae) from Java and Sumatra, Indonesia, with implication of sibling speciation. Med. Entomol. Zool. 47: 139–144.

    Google Scholar 

  • Hadi, U.K., H. Takaoka & C. Aoki, 1996c. Larval polytene chromosomes of three Japanese blackfly species (Diptera: Simuliidae). Med. Entomol. Zool. 47: 47–54.

    Google Scholar 

  • Hirai, H., W.S. Procunier, J.O. Ochoa & K. Uemoto, 1994. A cytogenetic analysis of the Simulium ochraceum species complex (Diptera: Simuliidae) in Central America. Genome 37: 36–53.

    Google Scholar 

  • Kuvangkadilok, C., S. Phayuhasena & C. Boonkemtong, 1999a. Larval polytene chromosomes of five Simulium species (Diptera: Simuliidae) from Doi Inthanon National Park, northern Thailand. Cytologia 64: 197–207.

    Google Scholar 

  • Kuvangkadilok, C., S. Phayuhasena & V. Baimai, 1999b. Population cytogenetic studies on Simulium feuerborni Edwards (Diptera: Simuliidae) from northern Thailand. Genome 42: 80–86.

    Google Scholar 

  • Kuvangkadilok, C. & H. Takaoka, 2000. Taxonomic notes on Simuliidae (Diptera) from Thailand: description of a new species and new distributional records of nine known species. Jpn. J. Trop. Med. Hyg. 28: 167–175.

    Google Scholar 

  • Maegga, B.T.A. & E.W. Cupp, 1994. Cytotaxonomy of the Simulium damnosum complex and description of new cytotypes in the Tukuyu focus southwest Tanzania. Trop. Med. Parasitol. 45: 125–129.

    Google Scholar 

  • Mafugai, H.B., R.J. Post, C.G. Vajime & D.H. Molyneux, 1996. Cytotaxonomic identification of the Simulium damnosum complex (Diptera: Simuliidae) from Nigeria. Trop. Med. Int. Health 1: 779–785.

    Google Scholar 

  • Millest, A.L., 1992. Identification of members of the Simulium ochraceum complex in the three onchocerciasis foci in Mexico. Med. Vet. Ent. 6: 23–28.

    Google Scholar 

  • Porter, D.L. & J. Martin, 1977. The cytology of Polypedilum nubifer (Diptera: Chironomidae). Caryologia 30: 41–62.

    Google Scholar 

  • Rothfels, K.H., 1956. Black flies: siblings, sex, and species grouping. J. Hered. 47: 113–122.

    Google Scholar 

  • Rothfels, K.H., 1979. Cytotaxonomy of black flies (Simuliidae). Annu. Rev. Entomol. 24: 507–539.

    Google Scholar 

  • Rothfels, K.H., 1980. Chromosomal variability and speciation in blackflies. Symp. Roy. Entomol. Soc. London 10: 207–224.

    Google Scholar 

  • Rothfels, K.H., 1981. Cytotaxonomy: principles and their application to some northern species-complexes in Simulium, pp. 19–29 in Blackflies: the Future for Biological Method in Integrated Control, edited by M. Laird. Academic Press Inc. (London) Ltd.

    Google Scholar 

  • Rothfels, K.H., 1989. Speciation in blackflies. Genome 32: 500–509.

    Google Scholar 

  • Rothfels, K.H. & R.W. Dunbar. 1953. The salivary gland chromosomes of the black flySimulium vittatum Zett. Can. J. Zool. 31: 226–241.

    Google Scholar 

  • Takaoka, H., 1979. The black flies of Taiwan (Diptera: Simuliidae). Pacific Insects 203: 65–403.

    Google Scholar 

  • Takaoka, H., 1996. The geographical distribution of the genus Simulium Latreille in the Oriental and Australasian regions. Jpn. Trop. Med. Hyg. 24: 113–124.

    Google Scholar 

  • Takaoka, H. & P.H. Adler, 1997. A new subgenus, Simulium (Daviesellum), and a new species, S. (D.) courtneyi, (Diptera: Simuliidae) from Thailand and Peninsular Malaysia. Jpn. J. Trop. Med. Hyg. 25: 17–27.

    Google Scholar 

  • Takaoka, H. & D.M. Davies, 1995. The Black Flies (Diptera: Simuliidae) of West Malaysia. Kyushu University Press, Fukuoka.

    Google Scholar 

  • Takaoka, H. & D.M. Davies, 1996. The Black Flies of Java, Indonesia. Bishop Museum Bull. Entomol. 6, Honolulu.

  • Takaoka, H. & K. Saito, 1996. A new species and new records of black flies (Diptera: Simuliidae) from Thailand. Jpn. J. Trop. Med. Hyg. 24: 163–169.

    Google Scholar 

  • Takaoka, H. & H. Suzuki, 1984. The blackflies (Diptera: Simuliidae) from Thailand. Jpn. J. Sanit. Zool. 35: 7–45.

    Google Scholar 

  • Vajime, C.G., 1989. Cytotaxonomy of Sirba form populations of the Simulium damnosum complex in West Africa: amendments to sex chromosomes and sibling status. Trop. Med. Parasitol. 40: 464–467.

    Google Scholar 

  • Vajime, C.G., P.A. Tambala, A. Kruger & R.J. Post, 2000. The cytotaxonomy of Simulium damnosum s.l. (Diptera: Simuliidae) from the Thyolo onchocerciasis focus in Malawi and description of a new member of the complex. Ann. Trop. Med. Parasitol. 94: 279–290.

    Google Scholar 

  • Wülker, W.F. & J. Martin, 1971. Karyosystematics of the Chironomus staegeri group. Stud. Nat. Sci. 1: 22–34.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, 10400, Thailand

    Chaliow Kuvangkadilok, Suwannee Phayuhasena & Visut Baimai

  2. National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Rama VI Road, Bangkok, 10400, Thailand

    Chainarong Boonkemtong

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  1. Chaliow Kuvangkadilok
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  2. Chainarong Boonkemtong
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  3. Suwannee Phayuhasena
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  4. Visut Baimai
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Kuvangkadilok, C., Boonkemtong, C., Phayuhasena, S. et al. Larval Polytene Chromosomes of Black Flies (Simulium) from Thailand. Genetica 118, 69–81 (2003). https://doi.org/10.1023/A:1022909018987

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