biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:199-203, 2003 | DOI: 10.1023/A:1022890208500

Bulblet Formation from Bulbscale Segments of Lilium Using Bioreactor System

M.L. Lian1, D. Chakrabarty1, K.Y. Paek1
1 Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, Korea

In vitro bulblet formation was studied using solid, liquid and bioreactor culture (immersion and periodic immersion in liquid media using ebb and flood) in order to develop a cost effective method for the mass propagation of Lilium oriental hybrid 'Casablanca'. Although the percent of bulblet formation was higher in solid culture, the increased growth rate and production of large number of bulblets in bioreactor makes it suitable for mass propagation. Four times per day and 15 min of medium supply was optimal for bulblet formation in ebb and flood bioreactor. Bulblet formation was also found to be effective in 16-h photoperiod. It was also observed that bulblet formation in the medium with 1.0 mg dm-3 BA and 0.3 mg dm-3 NAA was higher than in the medium without growth regulators, but formation of abnormal bulblets was higher in medium with BA and NAA.

Keywords: ebb and flood; liquid culture; lily; micropropagation; photoperiod; solid culture
Subjects: bioreactor; bulblet formation from bulbscale segments; daylength; in vitro culture, micropropagation; Lilium auratum; Lilium nobillissimum; Lilium oriental hybrid 'Casablanca'; Lilium rubellum; Lilium speciosum; lily, in vitro culture; photoperiod, in vitro culture, bioreactor

Prepublished online: March 1, 2003; Published: September 1, 2003  Show citation

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Lian, M.L., Chakrabarty, D., & Paek, K.Y. (2003). Bulblet Formation from Bulbscale Segments of Lilium Using Bioreactor System. Biologia plantarum, 46(2), 199-203. doi: 10.1023/A:1022890208500
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