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Optimization of cell growth and poly(glutamic acid) production in batch fermentation by Bacillus subtilis

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Abstract

Poly(glutamic acid) was produced maximally by Bacillus subtilis in batch fermentations at pH 7 and using glycerol at 20 g l−1 in a glutamic acid/citric acid medium. Poly(glutamic acid) reached 23 g l−1 after 30 h.

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References

  • Chemical and Engineering News (2002) States Sue Bristol-Myers over taxol generics. 80: 13.

  • Cromwick A-M, Birrer G, Gross R (1996) Effects of pH and aeration on ?-poly(glutamic acid) formation by Bacillus licheniformis in controlled batch fermentor cultures. Biotechnol. Bioeng. 50: 222–227.

    Google Scholar 

  • Engel J, Deger W, Reissman T, Losse G, Naumann W, Murgas S (2000) Process for the preparation of immobilized and activity stabilized complexes of LHTH. US Patent No. 6,054,555.

  • Giannos S, Gross A (1990) Poly(glutamic acid) produced by bacterial fermentation. In: Kaplan D, Mayer J, eds. Novel Biodegradable Microbial Polymers. Kluwer Academic Publishers, Dordrecht, pp. 457–460.

    Google Scholar 

  • Goto A, Kunioka M(1992) Biosynthesis and hydrolysis of poly(?-glutamic acid) from Bacillus subtilis IFO3335. Biosci. Biotech. Biochem. 56: 1031–1035.

    Google Scholar 

  • Hwan Do J, Chang H, Lee S (2001) Efficient recovery of poly(glutamic acid) from highly viscous culture broth. Biotechnol. Bioeng. 76: 219–223.

    Google Scholar 

  • Iwata H, Matsuda S, Mitsuhashi K, Kenji I, Itoh E, Ikada Y (1998) A novel surgical glue composed of gelatin and Nhydroxysuccinimide activated poly(L-glutamic acid). Biomaterials 19: 1869–1876.

    Google Scholar 

  • Ko Y, Gross R (1998) Effects of glucose and glycerol on ?-poly(glutamic acid) formation by Bacillus licheniformis ATCC 9945a. Biotechnol. Bioeng. 57: 430–437.

    Google Scholar 

  • Kubota H, Matsunobu T, Uotani K, Takebe H, Satoh A, Tanaka T, Taniguchi M (1993) Production of poly(glutamic acid) by Bacillus subtilis F-2-01. Biosci. Biotech. Biochem. 57: 1212–1213.

    Google Scholar 

  • Kunioka M, Goto A (1994) Biosynthesis of poly(?-glutamic acid) from L-glutamic acid, citric acid, and ammonium sulfate in Bacillus subtilis IFO3335. Appl. Microbiol. Biotechnol. 40: 867–872.

    Google Scholar 

  • Li C, Ke S, Wu Q-P, Tansey W, Hunter N, Buchmiller L, Milas L, Chamsangavej C, Wallace S (2000) Experimental therapeutics, preclinical pharmacology – Tumor irradiation enhances the tumor-specific distribution of poly(L-glutamic acid)-conjugated paclitaxel and its antitumor efficacy. Clin. Cancer. Res. 6: 2829–2834.

    Google Scholar 

  • Li C, Wallace S, Yu D-F, Yang D (1999) Water soluble paclitaxel prodrugs. US Patent No. 5,977,163.

  • Multani A, Li C, Ozen M, Yadav M, Yu D-F, Wallace S, Pathak S (1997) Paclitaxel and water-soluble poly(L-glutamic acid)-paclitaxel, induce direct chromosomal abnormalities and cell death in a murrine metastatic melanoma cell line. Anticancer Res. 17: 4269–4274.

    Google Scholar 

  • Myers A, Bichon D (1992) Cytotoxic drug conjugates and their delivery to tumor cells. US Patent No. 5,087,616.

  • Otani Y, Tabata Y, Ikada Y (1996) A new biological glue from gelatin and poly(L-glutamic acid). J. Biomed. Mat. Res. 31: 157–165.

    Google Scholar 

  • Otani Y, Tabata Y, Ikada Y (1999) Sealing effect of rapidly curable gelatin-poly(L-glutamic acid) hydrogel glue on lung air leak. Ann. Thorac. Surg. 67: 922–926.

    Google Scholar 

  • Richard A, Margaritis A (2001) Poly(glutamic acid) for biomedical applications. CRC Crit. Rev. Biotechnol. 21: 219–232.

    Google Scholar 

  • Richard A, Margaritis A (2003) Rheology, oxygen transfer and molecular weight characteristics of poly(glutamic acid) fermentation by Bacillus subtilis. Biotech. Bioeng., in press.

  • Sakurai Y, Okano T, Kataoka K, Yamada N, Inoue S, Yokoyama M (1997) Water soluble high molecular weight polymerized drug preparation. US Patent No. 5,693,751.

  • Sgouras D, Duncan R (1994) Evaluation of poly(glutamic acid, alanine, tyrosine) (1:1:1) as a lung-specific drug delivery system I. Biocompatibility and studies on biodistribution in the rat. STP Pharm. Sci. 4: 87–94.

    Google Scholar 

  • Yoon S, Do J, Lee S, Chang H (2000) Production of poly-?-glutamic acid by fed-batch culture of Bacillus licheniformis. Biotechnol. Lett. 22: 585–588.

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, Ontario, Canada, N6A 5B9

    Andrew Richard & Argyrios Margaritis

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  1. Andrew Richard
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  2. Argyrios Margaritis
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Correspondence to Argyrios Margaritis.

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Richard, A., Margaritis, A. Optimization of cell growth and poly(glutamic acid) production in batch fermentation by Bacillus subtilis . Biotechnology Letters 25, 465–468 (2003). https://doi.org/10.1023/A:1022644417429

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  • DOI: https://doi.org/10.1023/A:1022644417429

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