Skip to main content
SpringerLink
Log in

Suppressive Impact of Glucosinolates in Brassica Vegetative Tissues on Root Lesion Nematode Pratylenchus neglectus

  • Published:
Journal of Chemical Ecology Aims and scope Submit manuscript

Abstract

The potential of tissue amendments from a variety of wild and cultivated Brassica spp. to kill the root lesion nematode (Pratylenchus neglectus) in soil was assessed in laboratory experiments. Soil amended with leaf tissues was highly nematicidal, killing 56.2–95.2% of exposed nematodes. Amendment with root tissues was less effective, causing 0–48.3% mortality. Little of the suppressive impact by leaf tissues could be related to either total contents or any individual glucosinolate as determined by HPLC analysis. While the levels of total glucosinolates within root amendments had a nonsignificant relationship to nematode mortality, levels of 2-phenylethyl glucosinolate within root amendments significantly correlated with nematode suppression (P < 0.001). Amendment of soils with equimolar levels of purified 2-phenylethyl isothiocyanate resulted in comparable levels of nematode mortality, suggesting that 2-phenylethyl glucosinolate has a role in the suppressive impact of Brassica spp. root tissues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  • Ashley, M. G., and Leigh, B. L. 1963. The action of metham-sodium in the soil. I. Development of an analytical method for the determination of methyl isothiocyanate residues in soil. J. Sci. Food Agric. 14:148–153.

    Google Scholar 

  • Bernard, E. C., and Montgomery-Dee, M. E. 1993. Reproduction of plant parasitic nematodes on winter rapeseed (Brassica napus spp. oleifera). J. Nematol. (Suppl.). 25(4S):863–868.

    Google Scholar 

  • Brabban, A. D., and Edwards, C. 1995. The effects of glucosinolates and their hydrolysis products on microbial growth. J. Appl. Bacteriol. 79:171–177.

    Google Scholar 

  • Bradow, J. M. 1991. Relationships between chemical structure and inhibitory activity of C6 through C9 volatiles emitted by plant residues. J. Chem. Ecol. 17:2193–2212.

    Google Scholar 

  • Brown, P., and Morra, M. 1995. Glucosinolate containing plant tissues as bioherbicides. J. Agric. Food Chem. 43:3070–3074.

    Google Scholar 

  • Doncaster, C. C. 1962. A counting dish for nematodes. Nematology 7:334–336.

    Google Scholar 

  • Donkin, S., Eiteman, M., and Williams, P. 1995. Toxicity of glucosinolates and their enzymic decomposition products to Caenorhabditis elegans. J. Nematol. 27:258–262.

    Google Scholar 

  • Drobnica, L., Zemanova, M., Nemec, P., Antos, K., Kristian, P., Stullerova, A., Knoppova, V., and Nemec, P., Jr. 1967. Antifungal activity of isothiocyanates and related compounds. Appl. Microbiol. 15:701–709.

    Google Scholar 

  • Fenwick, G. R., Heany, R. K., and Mullin, W. J. 1994. Glucosinolates and their breakdown products in food and food plants. CRC Crit. Rev. Food Sci. Nutr. 18:123–201.

    Google Scholar 

  • Giamoustaris, A., Magrath, R., and Mithen, R. 1994. Modifying the aliphatic glucosinolate content of oilseed rape and its effect upon pest and pathogen interactions. Aspects Appl. Biol. 39:125–131.

    Google Scholar 

  • Glenn, M. G., Chew, F. S., and Williams, P. H. 1988. Influence of glucosinolate content of Brassica (Cruciferae) roots on growth of vesicular-arbuscular mycorrhizal fungi. New Phytol. 110:217–225.

    Google Scholar 

  • Heany, R., Spinks, E., Hanley, A., and Fenwick, G. 1986. Analysis of glucosinolates in rapeseed. Technical Bulletin. AFRC Food Research Institute, Norwick, UK.

    Google Scholar 

  • Horakova, K. 1966. Cytotoxicity of natural and synthetic isothiocyanates. Naturwissenschaften 53:383–384.

    Google Scholar 

  • Jiang, Y., Ghisalberti, E. L., and Ridsdell-Smith, T. J. 1996. Correlation of 1-octen-3-one with antixenotic resistance in subterranean clover cotyledons to red-legged earth mite, Halotydeus destructor (Acarina: Penthaleidae). J. Chem. Ecol. 22:369–382.

    Google Scholar 

  • Johnson, A. W., Golden, A. M., Auld, D. L., and Sumner, D. R. 1992. Effects of rapeseed and vetch as green manure crops and fallow on nematodes and soil-borne pathogens. J. Nematol. 24:117–126.

    Google Scholar 

  • Kawakishi, S., Goto, T., and Namiki, M. 1983. Oxidative scission of the disulfide bond of cysteine and polypeptides by the action of allyl isothiocyanate. Agric. Biol. Chem. 47:2071–2076.

    Google Scholar 

  • Kirkegaard, J., Gardner, P., Angus, J., and Koetz, E. 1994. Effect of Brassica break crops on the growth and yield of wheat. Aust. J. Agric. Res. 45:529–545.

    Google Scholar 

  • Lazzeri, L., Tacconi, R., and Palmieri, S. 1993. In vitro activity of some glucosinolates and their reaction products towards a population of the nematode, Heterodera schachtii. J. Agric. Food Chem. 41:825–829.

    Google Scholar 

  • Matthiesson, J. N., Desmarchilier, J. M., Vu, L. T., and Shackleton, M. A. 1996. Comparative efficacy of fumigants against hatching Whitefringed beetle (Coleoptera: Curculionidae) larvae and their sorption by soil. J. Econ. Entomol. 89:1372–1378.

    Google Scholar 

  • Mayton, H. S., Olivier, C., Vaughn, S. F., and Loria, R. 1996. Correlation of fungicial activity of Brassica species with allyl isothiocyanate production in macerated leaf tissue. Phytopathology 86:267–271.

    Google Scholar 

  • McBeth, C., and Bergeson, G. 1953. Methods of assaying nematicides. Methods Phytopathol. 43:264–267.

    Google Scholar 

  • McFadden, W., Potter, J., and Brandle, J. E. 1992. Use of Brassica spp. as a green manure for biological control of Pratylenchus penetrans. Phytopathology 82:246.

    Google Scholar 

  • Mithen, R. 1992. Leaf glucosinolate profiles and their relationship to pest and disease resistance in oilseed rape. Euphytica 63:71–83.

    Google Scholar 

  • Mithen, R., Raybould, A. F., and Giamoustaris, A. 1995. Divergent selection for secondary metabolites between wild populations of Brassica oleracea and its implications for plant-herbivore interactions. Heredity 75:472–484.

    Google Scholar 

  • Mojtahedi, H., and Santo, G. S. 1996. Toxicity of isothiocyanate derivatives to Meloidogyne chitwoodi and Pratylenchus penetrans in soil environment. Proceedings of the Third International Nematology Congress, Guadaloup, pp. 128–129.

  • Mojtahedi, H., Santo, G., Wilson, J., and Hang, A. 1993. Managing Meloidogyne chitwoodi on potato with rapeseed as green manure. Plant Dis. 77:42–46.

    Google Scholar 

  • Nicol, J. M., and Vanstone, V. 1993. Carrot piece and chickpea culture of Pratylenchus thornei and P. neglectus. Proceedings of the Pratylenchus Workshop, 9th Biennial Conference of the Australian Plant Pathology Society, Hobart, Australia.

  • Parker, G. 1994. Effect of canola (B. napus) on populations of Pratylenchus neglectus in the soil. Honors degree thesis. University of Adelaide, South Australia.

    Google Scholar 

  • Potter, T. 1995. Canola in South Australia. Proceedings of the 10th Australian Research Assembly on Brassicas. T. Potter (ed.). Struan, South Australia, pp. 6–7.

    Google Scholar 

  • Sang, J. P., Minchinton, I. R., Johnstone, P. K., and Truscott, R. J. W. 1984. Glucosinolate profiles in the seed, root and leaf tissue of cabbage, mustard, rapeseed, radish and swede. Can. J. Plant Sci. 64:77–93.

    Google Scholar 

  • Somda, I., Brun, H., Chevre, A. M., and Renard, M. 1995. Variability of Leptosphaeria maculans towards Brassica juncea resistance introduced into Brassica napus, pp. 1254–1256, in D. Murphy (ed.). Proceedings of the Ninth International Rapeseed Congress. Rapeseed Today and Tomorrow, Vol. 4, K18. Organising Committee of the Ninth International Rapeseed Congress, Cambridge, UK.

  • Southey, J. F. (ed.). 1986. Laboratory Methods for Work with Plant and Soil Nematodes, 6th ed. Ministry of Agriculture, Fisheries and Food reference book, HMSO, London.

    Google Scholar 

  • Spak, J., Kolarova, J., Lewis, J., and Fenwick, G. R. 1993. The effect of glucosinolates (mustard oil glycosides) and products of their enzymatic degradation on the infectivity of turnip mosaic virus. Biol. Plant. 35:73–80.

    Google Scholar 

  • Thierfelder, A., and Friedt, W. 1995. Development of novel rapeseed varieties (Brassica napus) resistant against beet cyst nematodes (Heterodera schachtii), pp. 1208–1210, in D. Murphy (ed.). Proceedings of the Ninth International Rapeseed Congress. Rapeseed Today and Tomorrow, Vol. 4, K2. Organising Committee of the Ninth International Rapeseed Congress, Cambridge, UK.

  • Underhill, E. W. 1980. Glucosinolates: Secondary plant products, pp. 493–511, in Encyclopedia of Plant Physiology, New Series. Vol. 8. E. A. Bell and B. V. Charlwood (eds.). Springer-Verlag, Berlin.

    Google Scholar 

  • Webb, R. 1990. Effects of nematode Pratylenchus fallax on roots of oilseed rape (Brassica napus var. oleifera). Rev. Nematol. 13:115–117.

    Google Scholar 

  • Williams, L., III, Morra, M. J., Brown, P. D., and McCaffrey, J. P. 1993. Toxicity of allyl isothiocyanate-amended soil to Limonius californicus (Mann.) (Coleoptera: Elateridae) wireworms. J. Chem. Ecol. 19:1033–1046.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Science, University of Adelaide, Waite Campus, PMB 1, Glen Osmond, South Australia

    Mark J. Potter & Anthony J. Rathjen

  2. Department of Crop Protection, University of Adelaide, Waite Campus, PMB 1, Glen Osmond, South Australia

    Kerrie Davies

Authors
  1. Mark J. Potter
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kerrie Davies
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Anthony J. Rathjen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Potter, M.J., Davies, K. & Rathjen, A.J. Suppressive Impact of Glucosinolates in Brassica Vegetative Tissues on Root Lesion Nematode Pratylenchus neglectus . J Chem Ecol 24, 67–80 (1998). https://doi.org/10.1023/A:1022336812240

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1022336812240

Search

Navigation