biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:101-105, 2003 | DOI: 10.1023/A:1022322403283

Growth and Enzyme Activity in Roots and Calli of Resistant and Susceptible Allium Lines as Influenced by Sterile Culture Filtrates of Phoma terrestris

D. Zappacosta1, R. Delhey1, N. Curvetto1
1 Department of Agronomy, Universidad Nacional del Sur, CERZOS (CONICET), Bahia Blanca, Argentina

Growth and activities of peroxidases, chitinases and glucanases were studied in order to evaluate the response of calli and roots of pink root-susceptible Allium cepa cvs. Valcatorce and T-412 and resistant A. fistulosum cv. Nogiwa Negi, to sterile culture filtrates of Phoma terrestris. Untreated calli and roots of A. fistulosum exhibited higher activity of peroxidases and glucanases than that of Valcatorce and T-412. Enzyme activities and growth of roots and calli were not affected in filtrate-treated A. fistulosum. The growth of calli and roots of A. cepa cultivars decreased significantly after exposure to P. terrestris filtrates while the peroxidase and glucanase activities increased. Peroxidase and glucanase activities were also enhanced in roots of Valcatorce bulbs grown in P. terrestris-inoculated soil as compared to healthy control plants. We conclude that a high constitutive activity of glucanases and perhaps chitinases might account for the resistance of A. fistulosum. The differential reaction (with respect to root growth) of pink root-susceptible and resistant materials to culture filtrates indicates that this in vitro-system might be useful for the screening of onion breeding lines.

Keywords: onion; Japanese bunching onion; fungal toxins; in vitro screening
Subjects: Allium cepa; bacteria; banana, defense systems induction; chitinase; defense enzymes induction, fusarium wilt, fluorescent pseudomonas; fluorescent pseudomonad, defense enzymes induction; fungal toxins; fungi; Fusarium oxysporum; β-1,3-glucanase; in vitro culture, screening of onion lines; Japanese bunching onion; Musa sp.; onion, resistant and susceptible lines; peroxidase; phenolics induction, fusarium wilt, fluorescent pseudomonas; Phoma terrestris; Pseudomonas fluorescens

Prepublished online: March 1, 2003; Published: July 1, 2003  Show citation

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Zappacosta, D., Delhey, R., & Curvetto, N. (2003). Growth and Enzyme Activity in Roots and Calli of Resistant and Susceptible Allium Lines as Influenced by Sterile Culture Filtrates of Phoma terrestris. Biologia plantarum, 46(1), 101-105. doi: 10.1023/A:1022322403283
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