Abstract
Oxidative status of rat cardiomyocytes during ischemia induced by occlusion of the descending branch of the coronary artery was studied by the methods of Fe-induced chemiluminescence and spectrophotometry of primary and secondary lipid peroxidation product. The concentrations of low-molecular-weight antioxidants a-tocopherol and citrate and activities of NADP-dependent isocitrate dehydrogenase (EC 1.1.1.41) and aconitate hydratase (EC 4.2.1.3) were also measured. Ischemia was associated with intensification of free radical processes, increased antioxidant activity in subcellular fractions of the myocardium, activation of NADP-isocitrate dehydrogenase, accumulation of citrate, and inhibition of aconitate hydratase. Differential centrifugation, ion exchange chromatography on various ion exchangers, and electrophoresis in polyacrylamide gel revealed no redistribution of enzyme activity between the cytoplasmic and mitochondrial cardiomyocyte fractions during ischemia.
Similar content being viewed by others
REFERENCES
Zh. I. Abramova and G. I. Oksengendler, Man and Antioxidants [in Russian], Leningrad (1985).
V. G. Afanas'ev, V. S. Zaitsev, and T. I. Vol'fson, Lab. Delo, No. 1, 115–116 (1973).
Yu. Brawnvald, P. R. Maroco, and P. Libby, Myocardium Metabolism, Ed. E. M. Chazov, Moscow (1975).
A. I. Kuz'menko, R. P. Morozova, I. A. Nikolenko, et al., Biokhimiya, 62, 712–715 (1997).
A. N. Osipov, O. A. Azizova, and Yu. A. Vladimirov, Uspekhi Biol. Khimii, 31, 180–204 (1990).
V. P. Skulachev, Biokhimiya, 64,No. 12, 16,679–16,688 (1999).
V. B. Spirichev, I. I. Matusis, and L. M. Bronshtein, Experimental Vitaminology, Ed. Yu. M. Ostrovskii [in Russian], Moscow (1979), pp. 36–37.
I. D. Stal'naya and T. G. Garishvili, Modern Methods in Biochemistry, Ed. V. N. Orekhovich [in Russian], Moscow (1977), pp. 63–68.
P. R. Gardner, D. M. Nguyen, and C. W. White, Proc. Natl. Acad. Sci. USA, 91,No. 25, 12,248–12,252 (1994).
D. Johnson and H. Lardy, Methods Enzymol., 10, 94–102 (1967).
O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. Randall, J. Biol. Chem., 194, 265–271 (1951).
A. Pastushko and A. Gromek, Bull. Acad. Pol. Sci. Ser. Sci. Biol., 24, 415–422 (1976).
M. Pellet, G. Baveret, and M. L. Mastin, Lyon Med., 230No. 19, 681–689 (1973).
R. Pfeifer, G. Rarl, and R. Scholz, Biol. Chem. Hopp-Seyler, 367, 1061–1068 (1986).
V. P. Skulachev, Biosci. Rep., 17No. 3, 347–366 (1997).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Medvedeva, L.V., Popova, T.N., Artyukhov, V.G. et al. Oxidative Status and Distribution of NADP-Dependent Isocitrate Dehydrogenase and Aconitate Hydratase in Rat Cardiomyocytes under Normal Conditions and during Ischemia. Bulletin of Experimental Biology and Medicine 134, 130–134 (2002). https://doi.org/10.1023/A:1021171811902
Issue Date:
DOI: https://doi.org/10.1023/A:1021171811902