Abstract
The structure of Latimeria chalumnae (coelacanth) proteolipid protein/DM20 gene excluding exon 1 was determined, and the amino acid sequence of Latimeria DM20 corresponding to exons 2–7 was deduced. The nucleotide sequence of exon 3 suggests that only DM20 isoform is expressed in Latimeria. The structure of proteolipid protein/DM20 gene is well preserved among human, dog, mouse, and Latimeria. Southern blot analysis indicates that Latimeria DM20 gene is a single-copy gene. When the amino acid sequences of DM20 were compared among various species, Latimeria was more similar to tetrapods than other fishes including lungfish, confirming the previous finding by immunoreactivity (Waehneldt and Malotka 1989 J. Neurochem. 52:1941–1943). However, when phylogenetic trees were constructed from the DM20 sequences, lungfish was clearly the closest to tetrapods. Latimeria was situated outside of lungfish by the maximum likelihood method. The apparent similarity of Latimeria DM20 to tetrapod proteolipid protein/DM20 is explained by the slow amino acid substitution rate of Latimeria DM20.
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REFERENCES
Lees, M. B., and Bizzozero, O. A. 1992. Structure and acylation of proteolipid protein. Pages 237–255, in Martenson, R. E. (ed.), Myelin: Biology and Chemistry, CRC Press, Boca Raton, Florida.
Macklin, W. B., Campagnoni, C. W., Deininger, P. L., and Gardinier, M. V. 1987. Structure and expression of the mouse myelin proteolipid protein gene. J. Neurosci. Res. 18: 383–394.
Nave, K.-A, Lai, C., Bloom, F. E., and Milner, R. J. 1987. Splice site selection in the proteolipid protein (PLP) gene transcript and primary structure of the DM-20 protein of central nervous system myelin. Proc. Natl. Acad. Sci. USA 84:5665–5669.
Simons, R., Alon, N., and Riordan, J. R. 1987. Human myelin DM-20 protolipid protein deletion defined by cDNA sequence. Biochem. Biophys. Res. Commun. 146:666–671.
Schliess, F., and Stoffel, W. 1991. Evolution of the myelin integral membrane proteins of the central nervous system. Biol. Chem. Hoppe-Seyler 372:865–874.
Waehneldt, T. V., Matthieu, J.-M., and Jeserich, G. 1986. Major central nervous system myelin glycoprotein of the African lungfish (Protopterus dolloi) cross-reacts with myelin proteolipid protein antibodies, indicating a close phylogenetic relationship with amphibians. J. Neurochem. 46:1387–1391.
Waehneldt, T. V., Matthieu, J.-M., Malotka, J., and Joss, J. 1987. A glycosylated proteolipid protein is common to CNS myelin of recent lungfish (Ceratodidae, Lepidosirenidae). Comp. Biochem. Physiol. 88:1209–1212.
Waehneldt, T. V., and Malotka, J. 1989. Presence of proteolipid protein in coelacanth brain myelin demonstrates tetrapod affinities and questions of a chondrichthyan association. J. Neurochem. 52:1941–1943.
Sambrook, J., Fritsch, E. F., and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd edit. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Betz, U. A. K., Mayer, W. E., and Klein, J. 1994. Major histocompatibility complex class I genes of the coelacanth Latimeria chalumnae. Proc. Nat. Acad. Sci. USA 91:11065–11069.
Sanger, F., Niklen, S., and Coulson, A. R. 1977. DNA sequencing with chain terminating inhibitors. Proc. Nat. Acad. Sci. USA 74:5463–5467.
Kishino, H., Miyata, T., and Hasegawa, M. 1990. Maximum likelihood inference of protein phylogeny and the origin of chloroplasts. J. Mol. Evol. 30:151–160.
Adachi, J., and Hasegawa, M. 1996. MOLPHY version 2.3: programs for molecular phylogenetics based on maximum likelihood. Computer Science Monographs, No. 28. Institute of Statistical Mathematics, Tokyo.
Saitou, N., and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406–425.
Diehl, H.-J., Schaich, M., Budzinski, R.-M., and Stoffel, W. 1986. Individual exons encode the integral membrane domains of human myelin proteolipid protein. Proc. Natl. Acad. Sci. USA 83:9807–9811.
Nadon, N. L., Duncan, I. D., and Hudson, L. D. 1990. A point mutation in the proteolipid protein gene of the ‘shaking pup’ interrupts oligodendrocyte development. Development 110:529–537.
Yoshida, M., and Colman, D. R. 1996. Parallel evolution and coexpression of the proteolipid proteins and protein zero in vertebrate myelin. Neuron 16:1115–1126.
Kitagawa, K., Sinoway, M. P., Yang, C., Gould, R. M., and Colman, D. W. 1993. A proteolipid protein gene family: expression in sharks and rays and possible evolution from an ancestral gene encoding a pore-forming polypeptide. Neuron 11:433–448.
Matthieu, J.-M., Eschmann, N., Bürgisser, P., Malotka, J., and Waehneldt, T. V. 1986. Expression of myelin proteins characteristic of fish and tetrapods by Polypterus revitalizes long discredited phylogenetic links. Brain Res. 379:137–142.
Macklin, W. B. (1992). The myelin proteolipid protein gene and its expression. Pages 257–276, in Martenson, R. E. (ed.), Myelin: Biology and Chemistry, CRC Press, Boca Raton, Florida.
Kurihara, T., Sakuma, M., and Gojobori, T. 1997. Molecular evolution of myelin proteolipid protein. Biochem. Biophys. Res. Commun. 237:559–561.
Tamai, Y., Kojima, H., and Abe, K. 1986. Chemical characterization of the brain of a coelacanth, Latimeria chalumnae. Comp. Biochem. Physiol. 83:295–299.
Waehneldt, T. V., Malotka, J., Jeserich, G., and Matthieu, J.-M. 1991. Central nervous system myelin proteins of the coelacanth Latimeria chalumnae: phylogenetic implications. Environ. Biol. Fishes 32:131–143.
Tamai, Y., Kojima, H., and Takayama-Abe, K. 1994. Lipid and myelin proteins in the brains of coelacanth (Latimeria chalumnae), lungfish (Lepidosiren paradoxa and Protopterus aetiopicus), bichir (Polypterus senegalus), and sturgeon (Acipenser ruthenus) (osteichthyes): phylogenetic implications. Can. J. Fish. Aquat. Sci. 51:1265–1272.
Romer, A. S. 1966. Vertebrate paleontology, 3rd edit. University of Chicago Press, Chicago.
Ahlberg, P. E., and Milner, A. R. 1994. The origin and early diversification of tetrapods. Nature 368:507–514.
Forey, P. L. 1988. Golden jubilee for the coelacanth Latimeria chalumnae. Nature 336:727–732.
Meyer, A. 1995. Molecular evidence on the origin of tetrapods and the relationships of the coelacanth. Trends Ecol. Evol. 10:111–116.
Zardoya, R., Cao, Y., Hasegawa, M., and Meyer, A. 1998. Searching for the closest relative(s) of tetrapods through evolutional analysis of mitochondrial and nuclear data. Mol. Biol. Evol. 15:506–517.
Cao, Y., Waddell, P. J., Okada, N., and Hasegawa, M. 1998. The complete mitochondrial DNA sequence of the shark Mustelus manazo: evaluating rooting contradictions to living bony vertebrates. Mol. Biol. Evol. 15:1637–1646.
Campagnoni, C. W., Kampf, K., Mason, B., Handley, V. W., and Campagnoni, A. T. 1994. Isolation and characterization of a cDNA encoding the zebra finch myelin proteolipid protein. Neurochem. Res. 19:1061–1065.
Hudson, L. D., Berndt, J. A., Puckett, C., Kozak, C. A., and Lazzarini, R. A. 1987. Aberrant splicing of proteolipid protein mRNA in the dysmyelinating jimpy mutant mouse. Proc. Natl. Acad. Sci. USA 84:1459–1458.
Milner, R. J., Lai, C., Nave, K.-A., Lenoir, D., Ogata, J., and Sutcliffe, J. G. 1985. Nucleotide sequence of two mRNAs for rat brain myelin proteolipid protein. Cell 42:931–939.
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Tohyama, Y., Kasama-Yoshida, H., Sakuma, M. et al. Gene Structure and Amino Acid Sequence of Latimeria chalumnae (Coelacanth) Myelin DM20: Phylogenetic Relation of the Fish. Neurochem Res 24, 867–873 (1999). https://doi.org/10.1023/A:1020958014398
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DOI: https://doi.org/10.1023/A:1020958014398