Abstract
The high molecular weight glutenin subunit (HMW-GS) composition of acollection of 107 Argentinean bread wheat cultivars was analysed bysodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE).Allelic variation at the Glu-1 loci was identified and its frequencycalculated. Eleven alleles were detected, three encoded at the Glu-A1locus, six at the Glu-B1 locus and two at the Glu-D1 locus. Alow frequency of the null allele at the Glu-A1 locus and a highfrequency of subunits 5+10 at the Glu-D1 locus were observed.Reversed phase-high performance liquid chromatography (RP-HPLC)analysis was used to further characterise HMW-GS. Two different types ofBx subunit 8 (named subunits 8 and 8) were detected, the latterhaving shorter elution time. Subunit 8 was not identifiable bySDS-PAGE. According to quantification by RP-HPLC analysis, two groupsof subunit 7 were observed. One group, with a relatively high proportionof subunit 7 (approximately 39% of the total amount of HMW-GS)appeared in cultivars with allele 7+8 at the Glu-B1 locus; asecond group of subunit 7 (around 24% of the total amount ofHMW-GS), was found in alleles 7+8, 7+8 and 7+9. Restrictionfragment length polymorphisms (RFLP) analyses of HMW-GS genes werealso carried out after digestion of genomic DNA with HindIII andTaqI restriction enzymes. The relationship between DNA fragment sizeand glutenin subunits, as estimated by electrophoretic mobility inSDS-PAGE, was also examined. The restriction enzyme TaqIdemonstrated to be a useful tool to detect homozygous plants in selectionprograms against the Glu-A1 null allele.
Article PDF
Similar content being viewed by others
References
Anderson, O.D, F.C. Green, R.E. Yip, N.G. Halford, P.R. Shewry & J.M. Malpica-Romero, 1989. Nucleotide sequences of the two high-molecular-weight glutenin genes from the D-genome of a hexaploid bread wheat, Triticum aestivum L. cv Cheyenne. Nucleic Acids Res 17: 461–462.
Branlard, G. & M. Dardevet, 1985. Diversity of grain protein and bread wheat quality. II. Correlation between high molecular subunits of glutenin and flour quality characteristics. J Cereal Sci 3: 345–354.
Cooke, R.J., 1995. Allelic variability at the Glu-1 loci in wheat varieties. Plant Var & Seeds 8: 97–106.
Dong, H., T.S. Cox, R.G. Sears & G.L. Lookhart, 1991. High molecular weight glutenin genes: effects on quality in wheat. Crop Sci 31: 974–979.
D'Ovidio, R., S. Masci, E. Porceddu & D.D. Kasarda, 1997. Duplication of the Bx7 high-molecular-weight glutenin subunit gene in bread wheat (Triticum aestivum L.) cultivar Red River 68. Plant Breed 116: 525–531.
Dvorak, J., P.E. McGuire & B. Cassidy, 1988. Apparent sources of the A genomes of wheats inferred from the polymorphism in abundance and restriction fragment length of repeated nucleotide sequences. Genome 30: 680–689.
Dubcovsky, J., A.F. Galvez & J. Dvorak, 1994. Comparison of the genetic organization of the early salt-stress-response gene system in salt-tolerant Lophopyrum elongatum and salt-sensitive wheat. Theor Appl Genet 87: 957–964.
Felsenburg, T., A.A. Levy, G. Galili & M. Feldman, 1991. Polymorphism of high molecular weight glutenins in wild tetraploid wheat: spatial and temporal variation in a native site. Israel J Botany 40: 451–479.
Graybosch, R.A., C.J. Peterson, J.H. Lee & D.R. Shelton, 1994. Effects of glutenin protein polymorphisms on breadmaking quality of winter wheats. Crop Sci 34: 628–635.
Gupta, R.B. & F. MacRitchie, 1991. A rapid one-step onedimensional SDS-PAGE procedure for analysis of subunit composition of glutenin in wheat. J Cereal Sci 14: 105–109.
Gupta, R.B., Y. Popineau, J. Lefebvre, M. Cornec, G.J. Lawrence & F. MacRitchie, 1995. Biochemical basis of flour properties in bread wheats. II Changes in polymeric protein formation and dough/gluten properties associated with the loss of low Mr or high Mr glutenin subunits. J Cereal Sci 21: 103–116.
Harberd, N.P., D. Bartels & R.D. Thompson, 1986. DNA restriction fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits of wheat. Biochem Genet 24: 579–596.
Johansson, E., P. Henriksson, G. Svensson & W.K. Heneen, 1993. Detection, chromosomal location and evaluation of the functional value of a novel high Mr glutenin subunit found in Swedish wheats. J Cereal Sci 17: 237–245.
Lawrence, G.J., 1986. High-molecular-weight glutenin subunit composition of Australian wheat cultivars. Aust J Agric Res 37: 125–133.
Lawrence, G.J. & K.W. Shepherd, 1980. Variation in glutenin protein subunits of wheat. Aust J Biol Sci 33: 221–233.
Lawrence, G.J., H.J. Moss, K.W. Shepherd & C.W. Wrigley, 1987. Dough quality of biotypes of eleven Australian wheat cultivars that differ in high-molecular-weight glutenin subunit composition. J Cereal Sci 6: 99–101.
Lawrence, G.J., F. MacRitchie & C.W. Wrigley, 1988. Dough and baking quality of wheat lines deficient in glutenin subunits controlled by the Glu-A1, Glu-B1 and Glu-D1 loci. J Cereal Sci 7: 109–112.
Lukow, O.M., P.I. Payne & R. Tkachuk, 1989. The HMW glutenin subunit composition of Canadian wheat cultivars and their association with bread-making quality. J Sci Food Agric 46: 451–460.
Mackie, A.M., E.S. Lagudah, P.J. Sharp & D. Lafiandra, 1996. Molecular and biochemical characterisation of HMW glutenin subunits from T. tauschii and the D genome of hexaploid wheat. J Cereal Sci 23: 213–225.
MacRitchie, F., D.L. du Cros & C.W. Wrigley, 1990. Flour polypeptides related to wheat quality. In: Y. Pomeranz (Ed.), Advances in Cereal Science and Technology, Vol. 10, pp. 79–146.
MacRitchie, F., 1992. Physicochemical properties of wheat proteins in relation to functionality. Adv Food Nutr Res 36: 1–87.
MacRitchie, F. & D. Lafiandra, 1997. Structure-function relationships of wheat proteins. In: S. Damodaran & A. Paraf (Eds.), Food Proteins and Their Applications, pp. 293–324.
Manifesto, M.M., A.R. Schlatter, H.E. Hopp, E.Y. Suárez & J. Dubcovsky, 2001. Quantitative evaluation of genetic diversity in wheat germplasm using molecular markers. Crop Sci 41: 682–690.
Marchylo, B.A., J.E. Kruger & D.W. Hatcher, 1989. Quantitative reversed-phase high-performance liquid chromatographic analysis of wheat storage proteins as a potential quality prediction tool. J Cereal Sci 9: 113–130.
Marchylo, B.A., O.M. Lukow & J.E. Kruger, 1992. Quantitative variation in high molecular weight glutenin subunit 7 in some Canadian wheats. J Cereal Sci 15: 29–37.
Margiotta, B., G. Colaprico, R. D'Ovidio & D. Lafiandra, 1993. Characterization of high Mr subunits of glutenin by combined chromatographic (RP-HPLC) and electrophoretic separation and restriction fragment length polymorphism (RFLP) analyses of their encoding genes. J Cereal Sci 17: 221–236.
Morgunov, A.I., R.J. Pena, J. Crossa & S. Rajaram, 1993. Worldwide distribution of the Glu-1 alleles in bread wheat. J Genet & Breed 47: 53–60.
Nakamura, H., A. Inazu & H. Hirana, 1999. Allelic variation in high-molecular-weight glutenin subunit loci of Glu-1 in Japanese common wheats. Euphytica 106: 131–138.
Nakamura, H., 2000. The association between high molecular weight glutenin subunit compositions and the bread-making quality of Chinese and Japanese hexaploid wheats. Aust J Agric Res 51: 371–375.
Ng, P.K.W, N.E. Pogna, F. Mellini & W. Bushuk, 1989. Glu-1 allele composition of the wheat cultivars registered in Canada. J Genet & Breed 43: 53–59.
Payne, P.I., 1987. The genetical basis of breadmaking quality in wheat. Aspects Appl Biol 15: 79–90.
Payne, P.I. & G.J. Lawrence, 1983. Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for the high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res Commun 11: 29–35.
Payne, P.I., K.G. Corfield & J.A. Blackman, 1981a. Correlation between the inheritance of certain high-molecular-weight subunits of glutenin and bread-making quality in progenies of six crosses of bread wheat. J Sci Food Agric 32: 51–60.
Payne, P.I., L.M. Holt & C.N. Law, 1981b. Structural and genetic studies on the high-molecular-weight subunits of wheat glutenin. Part I. Allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theor Appl Genet 60: 229–236.
Payne, P.I., M.A. Nightingale, A.F. Krattiger & L.M. Holt, 1987. The relationship between HMW glutenin subunit composition and the breadmaking quality of British-grown wheat varieties. J Sci Food Agric 40: 51–65.
Payne, P.I., L.M. Holt, A.F. Krattiger & J.M. Carrillo, 1988. Relationship between seed quality and HMW glutenin subunit composition determined using wheat grown in Spain. J Cereal Sci 7: 229–235.
Pogna, N.E., F. Mellini, A. Beretta & A. Dal Belin Peruffo, 1989. The high-molecular-weight glutenin subunits of common wheat cultivars grown in Italy. J Genet & Breed 43: 17–24.
Pogna, N.E., J.C. Autran, F. Mellini, D. Lafiandra & P. Feillet, 1990. Chromosome 1B-encoded gliadins and glutenin subunits in durum wheat: genetics and relationship to gluten strength. J Cereal Sci 11: 15–34.
Reddy, P. & R. Appels, 1993. Analysis of a genomic DNA segment carrying the wheat high-molecular-weight (HMW) glutenin Bx17 subunit and its use as an RFLP marker. Theor Appl Genet 85: 616–624.
Rogers, W.J., P.I. Payne & K. Harinder, 1989. The HMW glutenin subunit and gliadin composition of German-grown wheat varieties and their relationship with bread-making quality. Plant Breed 103: 89–100.
Rogers, W.J., T.E. Miller, P.I. Payne, J.A. Seekings, E.J. Sayers, L.M. Holt & C.N. Law, 1997. Introduction to bread wheat (Triticum aestivum L.) and assessment for bread-making quality of alleles from T. boeticum Boiss. ssp. thaoudar at Glu-A1 encoding two high-molecular-weight subunits of glutenin. Euphytica 93: 19–29.
Rooke, L., F. Bekes, R. Fido, F. Barro, P. Gras, A.S. Thatam, P. Barcelo, P Lazzeri & P.R. Shewry, 1999. Over-expression of a gluten protein in transgenic wheat results in greatly increased dough strength. J Cereal Sci 30: 115–120.
Shewry, P.R., N.G. Halford & A.S. Tatham, 1992. The high molecular weight subunits of wheat glutenin. J Cereal Sci 15: 105–120.
Sutton, K.H., 1991. Qualitative and quantitative variation among high molecular weight subunits of glutenin detected by Reversed-phase High-performance Liquid Chromatography. J Cereal Sci 14: 25–34.
Thair, M., T. Turchetta, R. Anwar & D. Lafiandra, 1996. Assessment of genetic variability in hexaploid wheat landraces of Pakistan based on polymorphism for HMW glutenin subunits. Genet Res Crop Evol 43: 211–220.
Tkachuk, R. & J. Mellish, 1980.Wheat cultivar identification by gel high voltage gel electrophoresis. Ann Tech Agric 29: 207–212.
Thompson, R.D., D. Bartels & N.P. Harberd, 1985. Nucleotide sequences of a gene from chromosome 1D of wheat encoding a HMW-glutenin subunit. Nucleic Acids Res 13: 6833–6846.
Uhlen, A.K., 1990. The composition of high molecular weight glutenin subunits in Norwegian wheats and their relation to bread-making quality. Norwegian J Agric Sci 4:1- 17.
Van Hintum, Th.J.L. & A. Elings, 1991. Assessment of glutenin and phenotypic diversity of Syrian durum wheat landraces in relation to their geographical origin. Euphytica 55: 209–215.
Zhong-hu, H., R.J. Peña & S. Rajaram, 1992. High molecular weight glutenin subunit composition of Chinese bread wheats. Euphytica 64: 11–20.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gianibelli, M., Echaide, M., Larroque, O. et al. Biochemical and molecular characterisation of Glu-1 loci in Argentinean wheat cultivars. Euphytica 128, 61–73 (2002). https://doi.org/10.1023/A:1020643702867
Issue Date:
DOI: https://doi.org/10.1023/A:1020643702867