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Potentiation of Cytotoxicity of Mitoxantrone Toward CHO-K1 Cells in Vitro by Dipyridamole

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Abstract

Dipyridamole (DP), a clinically used vasodilator and an antiplatelet compound, augmented the activity of the anticancer drug mitoxantrone (MXN) toward Chinese hamster ovary (CHO-K1) cells in culture. Clonogenic assays indicated that DP (1.0, 2.5, and 5.0 µM) decreased the survival of cells treated with MXN (5 to 25 nM) in a dose-dependent manner. Further, DP (1 and 5 nM) decreased the MXN concentration required for 50% inhibition of cell growth from 3.2 to 1.8 and from 3.0 to 0.5 nM, respectively, over a period of 3 days. DP (10 µM) increased the accumulation of MXN by 1.8-fold in exponentially growing cells exposed to MXN. The enhanced levels of MXN in CHO-K1 cells in the presence of the chemosensitizer may account for the potentiation of MXN-cytotoxicity by DP.

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Authors and Affiliations

  1. Department of Basic Pharmaceutical Sciences, College of Pharmacy, University of South Carolina, Columbia, South Carolina, 29208

    Pankaj B. Desai & Rajagopalan Sridhar

  2. Department of Radiation Therapy and Cancer Center, Howard University, 2041 Georgia Avenue, NW, Washington, D.C., 20060

    Rajagopalan Sridhar

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  1. Pankaj B. Desai
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  2. Rajagopalan Sridhar
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Desai, P.B., Sridhar, R. Potentiation of Cytotoxicity of Mitoxantrone Toward CHO-K1 Cells in Vitro by Dipyridamole. Pharm Res 9, 178–181 (1992). https://doi.org/10.1023/A:1018920903436

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