Abstract
Porous polyamide 4,6 membranes developed as semi-permeable and biocompatible membranes for the encapsulation of pancreatic islets were prepared by precipitation in water. Different membrane morphologies were obtained by varying the molecular weight of polyamide 4,6, the solvent evaporation time and the composition of the casting solution. Each membrane was submitted to differential scanning calorimetry and water flux measurements to study the total water content and the different kinds of water in correlation with its permeability performances. Their biocompatibility was first evaluated by a surface protein adsorption test. Of the various membranes, the one prepared by immersion in water after 5 minutes of solvent evaporation, of 15% KS200 polyamide 4,6 solution added with 1% of polyvinylpyrrolidone K30 seems to be the most promising. This membrane is characterized by a low adsorption of proteins, a high hydraulic coefficient and an asymmetric structure. Such a membrane represents a good candidate to be an efficient immunological barrier. It also exhibits good glucose and insulin diffusion properties. Moreover, rat islets cultivated on its surface were not affected by its presence and no important cell adhesion was noticed when implanted in mice. This membrane exhibits most of the properties suitable for the islet encapsulation with a view to developing a bioartificial pancreas.
Similar content being viewed by others
References
Diabetes Complication Trial Group, N. Engl. J. Med. 329 (1993) 977.
R. A. CUTHBERTSON, J. L. HOPPER and T. E. MANDEL, Transplant. 48 (1989) 218.
R. ALEJANDRO, A. TZAKIS, C. RICORDI, Y. ZENG, S. TODO, V. MAZZAFERRO and D. H. MINTZ, Transplant. Proc. 23 (1991) 789.
C. RICORDI, A. TZAKIS, P. B. CARROLLI, Y. ZENG, H. L. R. RILO, R. ALEJANDRO, R. SHAPIRO, J. J. FUNG, A. J. DMETRIS, D. H. MINTZ and T. E. STARZL, Transplant. 53 (1992) 407.
D. W. SCHARP, P. E. LACY, J. V. SANTIAGO, C. S. McCULLOUGH, L. G. WEIDE, P. J. BOYLE, L. FALQUI, P. MARCHETTI, C. RICORDI, R. L. GINGERICH, A. S. JAFFE, P. E. CRYER, D. W. HANTO, C. B. ANDERSON, and M. W. FLYE, ibid. 51 (1991) 76.
G. L. WARNOCK, N. W. KNETEMAN, E. A. RYAN, A. RABINOVITCH and R. V. RAJOTTE, Diabetologia 35 (1992) 29.
G. REACH, in “Biotechnology of insulin therapy” edited by J. C. Pickup (Blackwell, London, 1991) p. 76.
M. Y. FAN, Z. P. LUM, X. W. FU, L. LEVESAVE, I. J. TAI and A. M. SUN, Diabetes 39 (1990) 519.
J. J. ALTMAN, A. HOULBERT, P. CALLARD, P. McMILLAN, B. A. SOLOMON, J. ROSEN and P. M. GALETTI, ibid. 35 (1986) 625.
H. IWATA, T. TAKAGI, H. AMEMIYA, K. SHIMIZU, K. YAMASHITA, K. KOBAYASHI and T. AKUTSU, J. Biomed. Mater. Res. 26 (1992) 967.
G. F. KLOMP, H. HASHIGUCHI, P. C. URSELL, Y. TAKEDA, T. TUGUCHI and W. H. DOBELLE, ibid. 17 (1983) 865.
G. J. ZONDERVAN, H. J. HOPPEN, A. J. PENNINGS, W. FRITSCHY, G. WOLTERS and VAN SCHILF GAARDER, Biomaterials 13 (1992) 136.
P. E. LACY, O. D. HEGRE, F. T. GERASIMIDIVAZEOVA GENTILE and K. E. DIONNE, Science 254 (1991) 1782.
T. ZEKORN, U. SIEBERS, L. FILID, K. MAVER, U. SCHMITT, R. G. BRETZEL, and K. FEDERLIN, Transplant. Proc. 21 (1989) 2748.
A. CHIHANI, F. Z. BAZA and H. MENDJEL, Eur. Polym. J. 30 (1994) 711.
T. URAGAMI, Y. SUGITANI and M. SUGIHARA, Polymer 23 (1982) 192.
Y. TANIGUCHI, and S. HORIGOME, J. Appl. Polym. Sci. 19 (1975) 2743.
S. TAKIGAMI, T. KIMURA and Y. NAKAMURA, Polymer 34 (1993) 604.
B. A. MANSOR and B. H. MALCOLM, Polym. Int. 33 (1994) 273.
N. NISHIOKA, S. YOSHIMI, T. IWAGUCHI and K. KOSAI, Polym. J. 16 (1984) 877.
L. KESSLER, M. APRAHAMIAN, M. KEIPES, C. DAMGE, M. PINGET and D. POINSOT, Biomaterials 13 (1992) 44.
O. H. LOWRY, W. J. ROSEBROUGH, L. FARRA and R. J. RANDALL, J. Biol. Chem. 193 (1951) 265.
A. S. HUGGET and D. A. NIXON, Lancet ii(1957) 368.
P. E. LACY and M. KOSTIANOUSKY, Diabetes 16 (1967) 35.
J. MCGUIRE and V. KRISDHASIMA, in “Contact angle, wettability and adhesion”, (edited by K. L. Mittal), p. 829.
K. HOLMBERG, K. BERGSTRÖM, C. BRINK, E. ÖSTERBERG, F. TIBERG and J. MILTON HARRIS, in “Contact angle, wettability and adhesion”, (Edited by K. L. Mittal), p. 813.
M. S. SHEU, A. S. HOFFMAN and J. FEIJEN, in “Contact angle, wettability and adhesion”, (Edited by K. L. Mittal), p. 531.
L. KESSLER, M. PINGET, M. APRAHAMIAN, P. DEJARDIN and C. DAMGE, Horm. Metab. Res. 23 (1991) 312.
J. SMID, J. H. M. ALBERS and A. P. M. KUSTERS, J. Membr. Sci. 64 (1991) 121.
M. A. FROMMER and R. M. MESSALAM, Ind. Eng. Chem., Prod. Res. Dev. 12 (1973) 328.
H. STRATHMAN, K. KOCK, P. AMAR and R. W. BAKER, Desalination 16 (1975) 179.
C. A. SMOLDERS, A. J. REUVERS, R. M. BOOM and I. M. WIENK, J. Membr. Sci. 73 (1992) 259.
R. M. BOOM, I. M. WIENK, T. H. VAN DEN BOOMGAARD and C. A. SMOLDERS, ibid. 73 (1992) 277.
G. SOLDANI, P. GIUSTI, P. MARCHETTI, R. GIANNARELLI, A. DI CARLO and R. NAVALESI, J. Mater. Sci. Mater. Med. 3 (1992) 371.
T. ZEKORN, U. KOMP, R. G. BRETZEL and K. FEDERLIN, Horm. Metab. Res. 19 (1987) 87.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
LHOMMEAU , C., TOILLON , S., PITH , T. et al. Polyamide 4,6 membranes for the encapsulation of Langerhans islets: preparation, physico-chemical properties and biocompatibility studies. Journal of Materials Science: Materials in Medicine 8, 163–174 (1997). https://doi.org/10.1023/A:1018575220505
Issue Date:
DOI: https://doi.org/10.1023/A:1018575220505