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Location of respiration activity in filamentous bacteria by image analysis

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Biotechnology Techniques

Abstract

Respiration activity was located in Streptomyces ambofaciens using formazan crystals and image analysis. The technique was validated by comparing it to a global respiration rate measurement method. A uniform distribution of formazan crystals along the hyphae was found in cultivations in flasks and fermenter, throughout the cultures, although respiration is globally decreased when cultures age.

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Authors and Affiliations

  1. Laboratoire des Sciences du Génie Chimique CNRS-ENSIC-INPL, 1, rue Grandville, BP 451, F-54001, Nancy cedex, France

    P. Mauss, M.N. Pons & H. Vivier

  2. Laboratoire de Microbiologie Industrielle ENSAIA-INPL, 2 avenue de la Forêt de Haye, BP 172, F-54505, Vandoeuvre-les-Nancy cedex, France

    J.F. Drouin & P. Germain

  3. Rhône-Poulenc Rorer SA, 13 quai Jules Guesde, BP 14, F-94400, Vitry-sur-Seine, France

    B. Vanhoutte

  4. CRITT, Rhône-Poulenc, F-69000, Décines, France

    L. Louvel

Authors
  1. P. Mauss
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  2. J.F. Drouin
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  3. M.N. Pons
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  4. H. Vivier
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  5. P. Germain
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  6. L. Louvel
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  7. B. Vanhoutte
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Mauss, P., Drouin, J., Pons, M. et al. Location of respiration activity in filamentous bacteria by image analysis. Biotechnology Techniques 11, 813–817 (1997). https://doi.org/10.1023/A:1018477308962

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  • DOI: https://doi.org/10.1023/A:1018477308962

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