Abstract
The objective of this work was to evaluate the potential of near-infrared reflectance spectroscopy (NIRS) as a rapid method to estimate the fatty acid composition of the oil in intact-seed samples of rapeseed. A total of 549 samples (3 g intact seed) from selected mutant and breeding lines were scanned by NIRS, and 220 of them were selected and scanned again by using two different adapters, which reduced the sample size to 300 and 60 mg, respectively. Selected samples were analysed by gas liquid chromatography and calibration equations for individual fatty acids were developed. Calibrations for oleic, linoleic, linolenic, and erucic acid were highly accurate, with values of r2 in cross validation from 0.95 to 0.98 (samples of 3 g), from 0.93 to 0.97 (300 mg), and from 0.84 to 0.96 (60 mg). Calibrations for palmitic and stearic acid were less accurate, with values of r2 in cross validation always lower than 0.8, probably because of the narrow range available for these fatty acids. The accuracy of the calibration equations for eicosenoic acid was very low (r2 = 0.69 in 3 g samples), although improved equations were developed (r2 from 0.78 to 0.91) when the relationship between erucic and eicosenoic acid was taken into account. We conclude that NIRS is a powerful technique to estimate the fatty acid composition of the oil in rapeseed, provided that samples covering a wide range of fatty acid levels are available, with the advantage that such estimation is possible with few additional costs when NIRS is used for the determination of other seed quality traits.
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References
Daun, J.K., K.M. Clear & P. Williams, 1994. Comparison of three whole seed near-infrared analyzers for measuring quality components of canola seed. J Am Oil Chem Soc 71: 1063-1068.
Daun, J.K. & P.C. Williams, 1995. Use of NIR spectroscopy to determine quality factors in harvest survey of canola. In: Proc 9th Int Rapeseed Congr, Cambridge, UK, pp. 864-866. 4-7 July 1995, Henry Ling Limited, Dorchester.
Jönsson, R., 1977. Erucic-acid heredity in rapeseed (Brassica napusL. and Brassica campestrisL.). Hereditas 86: 159-170.
Koester, S. & C. Paul, 1989. Application of near infrared reflectance spectroscopy (NIRS) in breeding rapeseed for improved quality. In: Eucarpia (Eds.), Science for Plant Breeding (Book of Poster Abstracts, Vol II, XII EucarpiaCongress), poster 31-4. Paul Parey Publishers, Berlin and Hamburg.
Murphy, D.J., 1995. The use of conventional and molecular genetics to produce new diversity in seed oil composition for the use of plant breeders - progress, problems and future prospects. Euphytica 85: 433-440.
Murray, I., 1987. NIR spectra of homologous series of organic compounds. In: J. Hollo et al. (Ed.), NIR/NIT Spectroscopy, pp. 13-28. Akademiai Kiado, Budapest.
Reinhardt, T.-C. & G. Röbbelen, 1991. Quantitative analysis of fatty acids in intact rapeseed by near infrared reflectance spectroscopy. In: GCIRC (Ed.), Proc 8th Int Rapeseed Congr, Saskatoon, Canada, pp. 1380-1384. 9-11 July 1991.
Reinhardt, T.-C., C. Paul & G. Röbbelen, 1992. Quantitative analysis of fatty acids in intact rapeseed by NIRS. In: I. Murray & I. Cowe (Eds.), Making Light Work. Advances in Near Infrared Spectroscopy, pp. 323-327. VCH, London.
Röbbelen, G. & A. Nitsch, 1975. Genetical and physiological investigations on mutants for polyenoic fatty acids in rapeseed, Brassica napusL. I. Selection and description of new mutants. Z Pflanzenzüchtg 75: 93-105.
Roberts, C.A., R.E. Joost & G.E. Rottinghaus, 1997. Quantification of ergovaline in tall fescue by near infrared reflectance spectroscopy. Crop Sci 37: 281-284.
Rücker, B. & G. Röbbelen, 1995. Development of high oleic acid rapeseed. In: GCIRC (Ed.), Proc 9th Int Rapeseed Congr, Cambridge, UK, pp. 389-391. 4-7 July 1995, Henry Ling Limited, Dorchester.
Sato, T., S. Kawano & M. Iwamoto, 1991. Near infrared spectral patterns of fatty acid analysis from fats and oils. J Am Oil Chem Soc 68: 827-833.
Sato, T., Y. Takahata, T. Noda, T. Tanagisawa, T. Morishita & S. Sakai, 1995. Nondestructive determination of fatty acid composition of husked sunflower (Helianthus annusL.) seeds by near-infrared spectroscopy. J Am Oil Chem Soc 72: 1177-1183.
Shenk, J. & M.O. Westerhaus, 1993. Analysis of Agriculture and Food Products by Near Infrared Reflectance Spectroscopy. Infrasoft International, Port Matilda.
Thies, W., 1971. Schnelle und einfache Analysen der Fettsaurzusammensetzung in einzelnen RapsKotyledonen I. Gaschromatographische und papierchromatographische Methoden. Z Pflanzenzüchtg 65: 181-202.
Velasco, L., J.M. Fernández-Mart ínez, M. Del Rio & A. De Haro, 1995. The applicability of NIRS for estimating multiple seed quality components in Ethiopian mustard. In: GCIRC (Ed.), Proc 9th Int Rapeseed Congr, Cambridge, UK, pp. 867-869. 4-7 July 1995. Henry Ling Limited, Dorchester.
Velasco, L., J.M. Fernández-Mart ínez & A. De Haro, 1996. Screening Ethiopian mustard for erucic acid by near infrared reflectance spectroscopy. Crop Sci 36: 1068-1071.
Velasco, L., J.M. Fernández-Mart ínez & A. DeHaro, 1997. Determination of the fatty acid composition of the oil in intact-seed mustard by near-infrared reflectance spectroscopy. J Am Oil Chem Soc (in press).
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Velasco, L., Becker, H.C. Estimating the fatty acid composition of the oil in intact-seed rapeseed (Brassica napus L.) by near-infrared reflectance spectroscopy. Euphytica 101, 221–230 (1998). https://doi.org/10.1023/A:1018358707847
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DOI: https://doi.org/10.1023/A:1018358707847