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The use of visual marker genes as cell-specific reporters of Agrobacterium-mediated T-DNA delivery to wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.).

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Abstract

Transfer of T-DNA from Agrobacterium tumefaciens and A. rhizogenes to cells of wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) is demonstrated following the inoculation of immature embryos and immature embryo-derived callus. Agrobacterium T-DNA vectors containing the C1/Lc anthocyanin-biosynthesis regulatory genes, the gusA gene or a synthetic green fluorescent protein gene (sgfp-S65T) were constructed from original binary vectors. The visual T-DNA markers were used as cell-autonomous reporters of early Agrobacterium-mediated transformation events in the wheat and barley cells. This localization of the transformed cells revealed a non-random distribution throughout each embryo and callus piece.

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Authors and Affiliations

  1. The Norman Borlaug Institute for Plant Science Research, De Montfort University, Scraptoft, Leicester, LE7 9SU, U.K

    Alex C. McCormac, Huixia Wu, Yibing Wang, Ruiji Xu, Malcolm C. Elliott & Dong-Fang Chen

  2. Department of Forestry, Huazhong Agricultural University, Wuhan, Hubei, 430070, China

    Manzhu Bao

Authors
  1. Alex C. McCormac
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  2. Huixia Wu
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  3. Manzhu Bao
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  4. Yibing Wang
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  5. Ruiji Xu
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  6. Malcolm C. Elliott
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  7. Dong-Fang Chen
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McCormac, A.C., Wu, H., Bao, M. et al. The use of visual marker genes as cell-specific reporters of Agrobacterium-mediated T-DNA delivery to wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.).. Euphytica 99, 17–25 (1998). https://doi.org/10.1023/A:1018303102488

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