Skip to main content
SpringerLink
Log in

Suppressive effect of endogenous regucalcin on deoxyribonuclic acid synthesis in the nuclei of rat renal cortex

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

The effect of regucalcin, a regulatory protein of Ca2+ signaling, on deoxyribonucleic acid (DNA) synthesis activity in the nuclei isolated from rat renal cortex was investigated. The addition of calcium chloride (10–100 μM) in the reaction mixture containing the nuclei caused a significant decrease in DNA synthesis activity. Nuclear DNA synthesis activity was significantly raised in the presence of EGTA (1 mM), a chelator of Ca2+, indicating that nuclear Ca2+ has an inhibitory effect. Regucalcin (0.1–0.5 μM) added in the reaction mixture in the presence of either EGTA (1 mM) or calcium chloride (50 μM) had a significant inhibitory effect on nuclear DNA synthesis activity. The presence of anti-regucalcin monoclonal antibody (10–50 ng/ml) in the reaction mixture caused a significant increase in DNA synthesis activity. This increase was completely abolished by the addition of regucalcin (0.5 μM). The effect of anti-regucalcin monoclonal antibody in increasing DNA synthesis was enhanced in the presence of EGTA. Additionally, an inhibitory effect of calcium chloride (10 or 50 μM) was enhanced in the presence of anti-regucalcin monoclonal antibody (25 ng/ml). The present study demonstrates that endogenous regucalcin has a suppressive effect on DNA synthesis in the nuclei of rat renal cortex.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Cheung WY: Calmodulin plays a pivotal role in cellular regulation. Science 207: 19–27, 1980

    Google Scholar 

  2. Heizman CW, Hunziker W: Intracellular calcium-binding proteins:More sites than insights. Trends Biochem Sci 16: 98–103, 1991

    Google Scholar 

  3. Kraus-Friedman N, Feng L: The role of intracellular Ca2+ in the regulation of gluconeogenesis. Metabolism 42: 389–403, 1996

    Google Scholar 

  4. Yamaguchi M, Yamamoto T: Purification of calcium binding substancefrom soluble fraction of normal rat liver. Chem Pharm Bull 26: 1915–1918, 1978

    Google Scholar 

  5. Shimokawa N, Yamaguchi M: Molecular cloning and sequencing of the cDNA coding for a calcium-binding protein regucalcin from rat liver. FEBS Lett 327: 251–255, 1993

    Google Scholar 

  6. Yamaguchi M: Role of regucalcin in calcium signaling. Life Sci 66:1769–1780, 2000

    Google Scholar 

  7. Yamaguchi M: The role of regucalcin in nuclear regulation of regenerating liver. Biochem Biophys Res Commun 276: 1–6, 2000

    Google Scholar 

  8. Shimokawa N, Yamaguchi M: Expression of hepatic calcium-binding protein regucalcin mRNA is mediated through Ca2+/calmodulin in rat liver. FEBS Lett 316: 79–84, 1993

    Google Scholar 

  9. Yamaguchi M, Isogai M: Tissue concentration of calcium-binding protein regucalcin in rats by enzyme-linked immunoadsorbent assay. Mol Cell Biochem 122: 65–68, 1993

    Google Scholar 

  10. Yamaguchi M, Kurota H: Expression of calcium-binding protein regucalcin mRNA in the kidney cortex of rats: The stimulation by calcium administration. Mol Cell Biochem 146: 71–77, 1995

    Google Scholar 

  11. Kurota H, Yamaguchi M: Activatory effect of calcium-binding protein regucalcin on ATP-dependent calcium transport in the basolateral membranes of rat kidney cortex. Mol Cell Biochem 169: 149–156, 1997

    Google Scholar 

  12. Kurota H, Yamaguchi M: Regucalcin increases Ca2+-ATPase activity and ATP-dependent calcium uptake in the microsomes of rat kidney cortex. Mol Cell Biochem 177: 201–207, 1997

    Google Scholar 

  13. Xue JH, Takahashi H, Yamaguchi M: Stimulatory effect of regucalcin on mitochondrial ATP-dependent calcium uptake activity in rat kidney cortex. J Cell Biochem 80: 285–292, 2000

    Google Scholar 

  14. Kurota H, Yamaguchi M: Inhibitory effect of regucalcin on Ca2+/calmodulin-dependent protein kinase activity in rat renal cortex cytosol. Mol Cell Biochem 177: 239–243, 1997

    Google Scholar 

  15. Kurota H, Yamaguchi M: Inhibitory effect of calcium-binding protein regucalcin in protein kinase C activity in rat renal cortex cytosol. Biol Pharm Bull 21: 315–318, 1998

    Google Scholar 

  16. Morooka Y, Yamaguchi M: Suppressive role of endogenous regucalcinin the regulation of protein phosphatase activity in rat renal cortex cytosol. J Cell Biochem 81: 639–646, 2001

    Google Scholar 

  17. Omura M, Yamaguchi M: Inhibition of Ca2+/calmodulin dependent phosphatase activity by regucalcin in rat liver cytosol: Involvement of calmodulin binding. J Cell Biochem 71: 140–148, 1998

    Google Scholar 

  18. Jones DP, McConkey DJ, Nicotera P, Orrenius S: Calcium activated DNA fragmentation in rat liver nuclei. J Biol Chem 264: 6398–6403, 1989

    Google Scholar 

  19. Burton A: Study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonuceic acid. Biochem J 62: 315–323, 1956

    Google Scholar 

  20. Lowry OH, Rosebrough NH, Farr AL, Randall RF: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–273, 1951

    Google Scholar 

  21. Lynch WE, Brown RF, Umeda T, Langreth SG, Liberman I: Synthesis of deoxyribonucleic acid by isolated liver nuclei. J Biol Chem 245: 3911–3916, 1970

    Google Scholar 

  22. Katsumata T, Yamaguchi M: Inhibitory effect of calcium-binding protein regucalcin on protein kinase activity in the nuclei of regenerating rat liver. J Cell Biochem: 71, 569–576, 1998

    Google Scholar 

  23. Inagaki S, Yamaguchi M: Enhancement of protein tyrosine phosphatase activity in the proliferation of cloned rat hepatoma H4-II-E cells: Suppressiverole of endogenous regucalcin. Int J Mol Med 6: 323–328, 2000

    Google Scholar 

  24. Lynch WE, Umeda T, Uyeda M, Liberman I: Nature of the deoxyribonucleic acid made by isolated liver nuclei. Biochim Biophys Acta 287:28–37, 1972

    Google Scholar 

  25. Yamaguchi M, Kanayama Y: Calcium-binding protein regucalcin inhibits deoxyribonucleic acid synthesis in the nuclei of regenerating rat liver. Mol Cell Biochem 162: 121–126, 1996

    Google Scholar 

  26. Tsurusaki Y, Misawa H, Yamaguchi M: Translocation of regucalcin to rat liver nucleus: Involvement of nuclear protein kinase and protein phosphatase regulation. Int J Mol Med 6: 655–660, 2000

    Google Scholar 

  27. Morooka Y, Yamaguchi M: Inhibitory effect of regucalcin on protein phosphatase activity in the nuclei of rat kidney cortex. J Cell Biochem 83: 111–120, 2001

    Google Scholar 

  28. Hunter T: Protein kinases and phosphatase: The Yin and Yang of protein phosphorylation and signaling. Cell 80: 225–236, 1995

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka, 422-8526, Japan

    Yoshiko Morooka & Masayoshi Yamaguchi

Authors
  1. Yoshiko Morooka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masayoshi Yamaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Morooka, Y., Yamaguchi, M. Suppressive effect of endogenous regucalcin on deoxyribonuclic acid synthesis in the nuclei of rat renal cortex. Mol Cell Biochem 229, 157–162 (2002). https://doi.org/10.1023/A:1017920620495

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1017920620495

Search

Navigation