Abstract
The herb Lobelia inflata L. (Lobeliaceae) containspiperidine alkaloids. The main alkaloid is the pharmacologically-activelobeline. We have studied the effects of alkaloid precursor amino acids (lysineand phenylalanine) on the growth and alkaloid production of hairy root culturesof Lobelia inflata L. The hairy root clone 8009/h7transformed with Agrobacterium rhizogenes strain R 1601wascultivated on B5 solid medium containing lysine (Lys) and phenylalanine (Phe)both in the presence and absence of the growth regulators IAA and kinetin. Onthe medium containing hormones growth was delayed until day 14. The applicationof growth regulators to the B5 media containing amino acids either singly or incombination increased the biomass in all cases. The maximum dry weight wasachieved in a medium containing Phe + Lys and growthregulators. The highest lobeline level (36 μg/g) was detectedintissues cultivated on hormone-free medium containing Phe. In hormone-freemedia,lobeline production increased in the presence of either Phe or Lys comparedwiththe control, but the addition of both greatly decreased synthesis. In contrast,the addition of both amino acids to media supplemented with IAA and kinetinincreased lobeline production.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bálványos I., Szőke É. and Kursinszki L. 1998. Effect of magnesium on the growth and alkaloid production of Lobelia inflata L. hairy root cultures. In: Kiss S.A. (ed.), Magnesium and Interaction of Magnesium with Trace Elements. Hung. Chem. Soc., Budapest, pp. 358–361.
Bálványos I., Szőke É. and Kursinszki L. 2001. The effect of growth regulators on the biomass formation and lobeline production of Lobelia inflata L. hairy root cultures. Plant Growth Regulation 34: 339–345.
Gamborg O.K., Miller R.A. and Ojima K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exptl. Cell. Res. 50: 151–158.
Ishimaru K., Sadoshima S., Neera S., Koyama K., Takahashi K. and Shimomura K. 1992. A Polyacetylene gentiobioside from hairy roots of Lobelia inflata. Phytochemistry 31: 1577–1579.
Ishimaru K., Yonemitsu H. and Shimomura K. 1991. Lobetyolin and lobetyol from hairy root culture of Lobelia inflata. Phytochemistry 30: 2255–2257.
Krajewska A., Szőke É., Petri G., Botz L. and Szarvas T. 1987. Effect of new synthetic regulators on biomass and alkaloid production by callus tissues of Lobelia inflata L. Acta Bot. Hung. 33: 407–411.
Murashige T. and Skoog F. 1962. A revised medium for rapid growth and bioassays with tabacco tissue cultures. Physiol Plant 15: 473–497.
Szőke É., Krajewska A., Neszmélyi A., Pomázi A. and Mészáros A. 1992. In vitro multiplication of Lobelia inflata plants and their alkaloid production. Planta Med. 58: 625–626.
Szőke É. 1994. In vitro culture and the production of lobeline and other related secondary metabolites. In: Baja Y.P.S. (ed.), Biotechnology in Agriculture and Forestry 28, Medicinal and Aromatic Plants VII. Springer, Berlin, Heidelberg, pp. 289–327.
Szőke É., Neszmélyi A., Bálványos I. and Krajewska A. 1998. NMR characterisation of lobeline from Lobelia inflata tissue cultures. Medical Science Monitor 4: 15–19.
Yonemitsu H., Shimomura K., Satake M., Mochida S., Tanaka M., Endo T. et al. 1990. Lobeline production by hairy root culture of Lobelia inflata L. Plant Cell Rep. 9: 307–310.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bálványos, I., Szõke, É. & Kursinszki, L. The influence of amino acids on the lobeline production of Lobelia inflata L. hairy root cultures. Plant Growth Regulation 36, 241–244 (2002). https://doi.org/10.1023/A:1016574003070
Issue Date:
DOI: https://doi.org/10.1023/A:1016574003070