Abstract
A fluorescence measurement system and methods of data analysis were developed to measure rapid kinetics of transdermal transport in vitro. Three variations on the technique were demonstrated, where the receptor compartment concentration was determined by: 1) fluorescence measurements of aliquots removed at discrete time points, 2) continuous fluorescence measurements made directly in the receptor compartment using a custom-made fluorimeter cuvette as a permeation chamber, and 3) continuous fluorescence measurements made in a flow-through cuvette containing receptor solution continuously pumped from a flow-through permeation chamber. In each case, the measured signal was a convolution of the time-dependent molecular flux (the desired information) and the characteristic response of the measurement system. Algorithms for deconvolution of the signal were derived theoretically. For the most complicated case, (3), the experimental confirmation is shown here, proving a time resolution on the order of half a minute.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
R. L. Bronaugh and H. I. Maibach (eds.). Percutaneous Absorption, Mechanisms—Methodology—Drug Delivery. Marcel Dekker, New York, 1989.
J. Hadgraft and R. H. Guy (eds.). Transdermal Drug Delivery: Developmental Issues and Research Initiatives, Marcel Dekker, New York, 1989.
Z. Fehér, I. Kolbe, and E. Pungor. Application of flow-through techniques to drug dissolution studies. Analyst 116, 48–487 (1991).
M. Talukdar and J. A. Plaizier-Vercammen. Flow-through methods is advantageous for dissolution experiment of small dosage form. Pharmazie 47, 458–459 (1992).
E. R. Edelman, L. Brown, J. Taylor, and R. Langer. In vitro and in vivo kinetics of regulated drug release from polymer matrices by oscillating magnetic fields. J. Biomed. Mater. Res. 21, 339–353 (1987).
W. J. Addicks, G. L. Flynn, and N. Weiner. Validation of a flow-through diffusion cell for use in transdermal research. Pharm. Res. 4, 337–341 (1987).
S. W. Frantz, D. A. Dittenber, D. L. Eisenbrandt, and P. G. Watanabe. Evaluation of a flow-through in vitro skin penetration chamber method using acetone-deposited organic solids. J. Toxicol—Cut. & Ocular Toxicol. 9, 277–299 (1990).
J. Sclafani, J. Nightengale, P. Liu, and T. Kurihara-Bergstrom. Flow-through system effects on in vitro analysis of transdermal systems. Pharm. Res. 10, 1521–1526 (1993).
K. Knutson, S. L. Krill, W. J. Lambert, and W. I. Higuchi. Probing the structure of stratum corneum on the molecular level. In P. I. Lee and W. R. Good (eds.), Controlled-Release Technology, American Chemical Society, Washington D.C., 1987, pp. 241–266.
M. R. Prausnitz, V. G. Bose, R. Langer, and J. C. Weaver. Electroporation of mammalian skin: A mechanism to enhance transdermal drug delivery. Proc. Natl. Acad. Sci. USA 90, 10504–10508 (1993).
M. R. Prausnitz, U. Pliquett, R. Langer, and J. C. Weaver. Rapid temporal control of transdermal drug delivery by electroporation. submitted.
D. C. Chang, B. M. Chassy, J. A. Saunders, and A. E. Sowers. Guide to Electroporation and Electrofusion, Academic Press, New York, 1992.
J. C. Weaver. Electroporation: a general phenomenon for manipulating cells and tissues. J. Cell Biochem. 51, 426–435 (1993).
J. W. Furry. Preparation, Properties and Applications of Calcein in a Highly Pure Form. PhD Thesis, Iowa State University, Ames, IA, 1985.
Rights and permissions
About this article
Cite this article
Pliquett, U., Prausnitz, M.R., Chizmadzhev, Y.A. et al. Measurement of Rapid Release Kinetics for Drug Delivery. Pharm Res 12, 549–555 (1995). https://doi.org/10.1023/A:1016254013374
Issue Date:
DOI: https://doi.org/10.1023/A:1016254013374