Abstract
The application of protease as a laundry detergent additive from a newly isolated Nocardiopsis sp., isolated from a soil sample collected in Northeast Brazil is reported. The optimal pH and temperature for protease activity were pH 10.5 and 50 °C, respectively. The enzyme was stable in a long-term incubation, showed 73.5% of initial activity at pH 10.5 and 61.7% at pH 12.0 for 120 min. Approximately 60% of initial activity remained after 120 min at 50 °C or after 30 min at 80 °C. Almost 87% of enzyme activity was retained in the presence of 10% (v/v) of peroxide at 40 °C, after 1 h. The protease also was stable in the presence of oxidants and surfactants such as SDS, saponin, Tween 20 and Tween 80 after 30 min. In the presence of Omo®, the enzyme retained 64% of its activity at 40 °C for 1 h. An increase in the proteolytic activity (6–17%) was observed with K+, Na+, and Mg++ ions. At pH 8.0, the protease hydrolysed casein maximally (50 U/mg).
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Moreira, K., Albuquerque, B., Teixeira, M. et al. Application of protease from Nocardiopsis sp. as a laundry detergent additive. World Journal of Microbiology and Biotechnology 18, 309–315 (2002). https://doi.org/10.1023/A:1015221327263
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DOI: https://doi.org/10.1023/A:1015221327263