biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 45:121-124, 2002 | DOI: 10.1023/A:1015121111934

In Vitro Long-Term Storage of Date Palm

S.A. Bekheet1, H.S. Taha1, M.M. Saker1
1 Plant Cell and Tissue Culture Department, National Research Centre, Cairo, Egypt

A reliable method for the long-term conservation of date palm tissue cultures is described. In vitro shoot bud and callus culture were successfully stored for 12 months at 5°C in the dark. At this conditions high percent of cultures remained viable without serious signs of senescence. However, the growth rate decreased as storage period increased. The role of sorbitol as osmotic agent in storage was examined. Health shoot bud cultures were obtained after 6 months of storage on medium containing 40 g dm-3 sorbitol. This period extended for 9 months in case of callus cultures.

Keywords: osmotic stress; Phoenix dactylifera
Subjects: date palm, in vitro culture; in vitro culture, bud and callus induction and growth; in vitro culture, long-term storage of bud and callus culture; osmotic stress, storage of in vitro culture; Phoenix dactylifera

Published: March 1, 2002  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Bekheet, S.A., Taha, H.S., & Saker, M.M. (2002). In Vitro Long-Term Storage of Date Palm. Biologia plantarum, 45(1), 121-124. doi: 10.1023/A:1015121111934
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bajaj, Y.P.S.: Cryopreservation and international exchange of germplasm.-In: Sen, S.K., Giles, K.L. (ed.): Plant Cell Culture in Crop Improvement. Pp. 19-41. Plenum Press, New York 1983. Go to original source...
    2. Bekheet, S.A.: In vitro preservation of Asparagus officinalis.-Biol. Plant. 43: 179-183, 2000. Go to original source...
    3. Fletcher, P.J.: In vitro long-term storage of asparagus.-New Zeal. J. Crop hort. Sci. 22: 351-359, 1994. Go to original source...
    4. Grout, B.W.: In vitro conservation of germplasm.-In: Bhojwani, S.S. (ed.): Plant Tissue Culture. Applications and Limitations. Pp. 394-411. Elsevier, Amsterdam 1990. Go to original source...
    5. Snedecor, G.W., Cochran, W.G.: Statistical Methods. 6th Edition.-Iowa State University Press, Iowa 1967.
    6. Tisserat, B., Ulrich, J., Finkle, B.: Cryogenic preservation and regeneration of date palm tissue.-HortScience 16: 47-48, 1981. Go to original source...
    7. Wanas, W.H.: In vitro storage of proliferated apple rootstock shoot-tip cultures.-Ann. agr. Sci. Ain Shams Univ. 37: 501-510, 1992.
    8. Wanas, W.H., Callow, J.A., Withers, L.A.: Growth limitation for the conservation of pear genotypes.-In: Withers, L.A., Alderson, P.G. (ed.): Plant Tissue Culture and its Agricultural Applications. Pp. 285-289. Cambridge University Press, Cambridge 1986. Go to original source...
    9. Withers, L.A.: Biotechnology and plant genetic resources conservation.-In: Paroda, R.S., Arora, R.K. (ed.): Plant Genetic Resources, Conservation and Management. Concepts and Approaches. Pp. 273-297. International Board for Plant Genetic Resources, New Delhi 1991.

    Return to the content