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pH regulation of enzyme production in Aspergillus nidulans growing in aerobic batch fermenter

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Abstract

The physiological response to ambient pH on the regulation of the production of the xylanolytic enzyme complex was investigated using two modified strains of Aspergillus nidulans. Transcriptional gene fusions were constructed between the promoters xlnA and xlnB (alkaline and acid expressed, respectively) and the Aspergillus niger goxC (encoding glucose oxidase), and A. nidulans transformants possessing single integrations at the argB locus were selected. Changing the pH from 5.5 to 4.5 or 7 after induction resulted in differential expression of the homologous and heterologous proteins. Thus, versatile industrial strains capable of differentially producing mixtures of extracellular enzymes in response to ambient pH can be produced.

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Authors and Affiliations

  1. Planta Piloto del Departamento de Biotecnología, CSIC, IATA (Instituto de Agroquímica y Tecnología de Alimentos), Apartado Postal 73, 46100, Burjassot, Valencia, Spain

    J.M. Bruno-Bárcena & D. Ramón

  2. PROIMI (Planta Piloto de Procesos Industriales Microbiológicos), CONICET, Universidad Nacional de Tucumán, Av. Belgrano y Pje. Caseros, 4000, S. M. de Tucumán, Argentina

    M.E. Lucca & F. Siñeriz

  3. Departamento de Medicina Preventiva y Salud Pública, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universitat de Valencia, Avenida Vicent Andrés Estellés s/n, 46100, Burjassot, Valencia, Spain

    D. Ramón

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  1. J.M. Bruno-Bárcena
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  2. M.E. Lucca
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  3. F. Siñeriz
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  4. D. Ramón
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Bruno-Bárcena, J., Lucca, M., Siñeriz, F. et al. pH regulation of enzyme production in Aspergillus nidulans growing in aerobic batch fermenter. Biotechnology Letters 24, 567–572 (2002). https://doi.org/10.1023/A:1014868726188

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  • DOI: https://doi.org/10.1023/A:1014868726188

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