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Heterogeneous plastid genomes in anther culture-derived albino rice plants

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Abstract

Large-scale deletions in the plastid genome of albino plants derived fromanther culture of japonica rice have in previous studies been detected bySouthern blot analysis. In the present study, PCR was applied to detectlarge-scale deletions in the plastid genome of albino and green plantsgenerated from the anther culture of japonica-indica hybrid-derived DHlines. The rice plastid genome (134.5 kbp) was separated into sevenregions and each one (approximately 20 kbp) was amplified by long PCR. The plastid sequences of all 22 albino and 19 green plants were amplifiedat all the seven regions, indicating that large-scale deletions in the plastidgenome were not detected by long PCR. The concentrations of two plastidgenome regions were measured by competitive PCR. Four of 29 albinoplants analyzed showed different concentrations between the two regions,indicating that both deleted and complete plastid molecules were presentin each of the four albino plants. Such plastid genome heterogeneity wasnot detected in 17 green plants.

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References

  • Barkan, A., R. Voelker, J. Mendel-Hartvig, D. Johnson & M. Walker, 1995. Genetic analysis of chloroplast biogenesis in higher plants. Physiol Plant 93: 163–170.

    Google Scholar 

  • Barnes, W., 1994. PCR amplification of up to 35-kb DNA with high fidelity and high yield from ? bacteriophage templates. Proc Natl Acad Sci USA 91: 2216–2220.

    Google Scholar 

  • Chen, C., H. Tsay & C. Huang, 1991. Factors affecting androgenesis in rice (Oryza sativa L.). In: Y.P.S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry. Vol 14: Rice, pp. 193–215. Springer-Verlag, Berlin, Heidelberg.

    Google Scholar 

  • Cheng, S., C. Fockler, W. Barnes & R. Higuchi, 1994. Effective amplification of long targets from cloned inserts and human genomic DNA. Proc Natl Acad Sci USA 91: 5695–5699.

    Google Scholar 

  • Chiba, T., T. Harada, S. Goto, R. Ishikawa & M. Niizeki, 1996. Transcription of tRNA genes from a large-scale plastid DNA deletion clearly reveals the action of nuclear-encoded RNA polymerase in the plastid. J Plant Physiol 148: 652–656.

    Google Scholar 

  • Day, A. & T. Ellis, 1985. Deleted forms of plastid DNA in albino plants from cereal anther culture. Current Genet 9: 671–678.

    Google Scholar 

  • Day, A. & T. Ellis, 1984. Chloroplast DNA deletions associated with wheat plants regenerated from pollen: Possible basis for maternal inheritance of chloroplasts. Cell 39: 359–368.

    Google Scholar 

  • Ellis, T. & A. Day, 1986. A hairpin plastid genome in barley. EMBO J 5: 2769–2774

    Google Scholar 

  • Gillham, N., 1994. Evolution of organelle genomes. In: Organelle Genes and Genomes, pp. 92–109. Oxford University Press, New York.

    Google Scholar 

  • Gilliand, G., S. Perrin, K. Blanchard & H. Bunn, 1990. Analysis of cytokine mRNA and DNA: Detection and quantitation by competitive polymerase chain reaction. Proc Natl Acad Sci USA 87: 2725–2729.

    Google Scholar 

  • Güiderdoni, E., E. Galinato, J. Luistro & G. Vergara, 1992. Anther culture of tropical japonica ? indica hybrids of rice. Euphytica 62: 219–224.

    Google Scholar 

  • Harada, T., R. Ishikawa, M. Niizeki & K. Saito, 1992. Pollen-derived rice calli that have large deletions in plastid DNA do not require protein synthesis in plastids for growth. Mol Gen Genet 233: 145–150.

    Google Scholar 

  • Harada, T., T. Sato, D. Asaka & I. Mitsukawa, 1991. Large-scale deletions of rice plastid DNA in anther culture. Theor Appl Genet 81: 157–161.

    Google Scholar 

  • He, P., L. Shen, C. Lu, Y. Chen & L. Zhu, 1998. Analysis of quantitative trait loci which contribute to anther culturability in rice (Oryza sativa L.). Mol Breed 4: 165–172.

    Google Scholar 

  • Hiratsuka, J., H. Shimada, R. Whittier, T. Ishibashi, M. Sakamoto, M. Mori, C. Kondo, Y. Honji, C. Sun, B. Meng, Y. Li, A. Kanno, Y. Nishizawa, A. Hirai, K. Shinozaki & M. Sugiura, 1989. The complete sequence of the rice (Oryza sativa) chloroplast genome: Intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals. Mol Gen Genet 217: 185–194.

    Google Scholar 

  • Kurata, N., Y. Nagamura, K. Yamamoto, Y. Harushima, N. Sue, J. Wu, B.A. Antonio, A. Shomura, T. Shimizu, S.Y. Lin, T. Inoue, A. Fukuda, T. Shimano, Y. Kuboki, T. Toyama, Y. Miyamoto, T. Kirihara, K. Hayasaka, A. Miyao, L. Monna, H.S. Zhong, Y. Tamura, Z.X. Wang, T. Momma, Y. Umehara, M. Yano, T. Sasaki & Y. Minobe, 1994. A 300 kilobase interval genetic map of rice including 883 expressed sequences. Nat Genet 8: 365–372.

    Google Scholar 

  • Leon, P., A. Arroyo & S. Mackenzie, 1998. Nuclear control of plastid and mitochondrial development in higher plants. Annu Rev Plant Physiol Plant Mol Biol 49: 453–80.

    Google Scholar 

  • Liu, Y., N. Mori & K. Tsunewaki, 1990. Restriction fragment length polymorphism (RFLP) analysis in wheat. I. Genomic DNA library construction and RFLP analysis in common wheat. Jpn J Genet 65: 367–380.

    Google Scholar 

  • Maruta, K., T. Tsuchiya, S. Kikuchi & K. Oono, 1992. Splicing variability of a chloroplast gene expression in albino pollen plants in rice (in Japanese). Jpn J Breed 42 (suppl. 1) 238–239.

    Google Scholar 

  • Mouritzen, P. & P. Holm, 1994. Chloroplast genome breakdown in microspore cultures of barley (Hordeum vulgare L.) occurs primarily during regeneration. J Plant Physiol 144: 586–593.

    Google Scholar 

  • Quimio, C.A. & F.J. Zapata, 1990. Diallel analysis of callus induction and green-plant regeneration in rice anther culture. Crop Sci 30: 188–192.

    Google Scholar 

  • Sathish, P., O. Gamborg & M. Nabors, 1995. Rice anther culture: callus initiation and androclonal variation in progenies of regenerated plants. Plant Cell Rep 14: 432–436.

    Google Scholar 

  • Siebert, P. & J. Larrick, 1992. Competitive PCR. Nature 359: 557–558.

    Google Scholar 

  • Sugimoto, K. & Y. Takeoka, 1998. Genetic analysis of plant regeneration ability in anther culture of rice (Oryza sativa L.) Breed Sci 48: 115–121.

    Google Scholar 

  • Tsukahara, M., T. Hirosawa & H. Murayama, 1996. Effect of culture methods on the regeneration of albino rice (Oryza sativa L.) plantlets. Plant Cell Rep 15: 597–600.

    Google Scholar 

  • Yamagishi, M., M. Otani, M. Higashi, Y. Fukuta, K. Fukui, M. Yano & T. Shimada, 1998. Chromosomal regions controlling anther culturability in rice (Oryza sativa L.). Euphytica 103: 227–234.

    Google Scholar 

  • Yamagishi, M., M. Yano, Y. Fukuta, K. Fukui, M. Otani & T. Shimada, 1996. Distorted segregation of RFLP markers in regenerated plants derived from anther culture of an F1 hybrid of rice. Gene Genet Syst 71: 37–41.

    Google Scholar 

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  1. Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane, 690-8504, Japan

    Masumi Yamagishi

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  1. Masumi Yamagishi
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Yamagishi, M. Heterogeneous plastid genomes in anther culture-derived albino rice plants. Euphytica 123, 67–74 (2002). https://doi.org/10.1023/A:1014493316433

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