Abstract
Translocations of A chromosome-specific and B chromosome-specific satellite DNA were tracked by fluorescence in situ hybridisation from an irradiated M1 generation of an experimental population of rye (Secale cereale L.) to its M2 progeny. Although high frequencies of large structural rearrangements were detected in root-tip meristems of M1 plants, none was present at meiosis or in somatic cells of their progeny. These results are interpreted in terms of efficient "filtering" of translocations during vegetative development, and not in the more usual terms of meiosis presenting a physical barrier to structural variants. These observations highlight the fact that B–A translocations are not tolerated, and may explain why this form of chromosome mutation is largely absent from natural populations.
References
Cuadrado A, Jouve N (1997) Distribution of highly repeated DNA sequences in species of the genus Secale. Genome 40: 309–317.
Gerlach WL, Dyer TA (1980) Sequence organization of the repeating units in the nucleus of wheat which contain 5S rRNA genes. Nucleic Acids Res 11;8(21): 4851–4865.
Hasterok R, Jenkins G, Langdon T, Jones RN, Maluszynska J (2001) Ribosomal DNA is an effective marker of Brassica chromosomes. Theor Appl Genet 103: 486–490.
Jones RN, Rees H (1982) B-chromosomes. London: Academic Press.
Langdon T, Seago Ch, Jones RN et al. (2000) De novo evolution of satellite DNA on rye B chromosome. Genetics 154: 869–884.
Puertas MJ, Baeza F (1983) Translocations between A and B chromosomes or rye. In: Kew Chromosome Conference II. George Allen & Unwin, pp. 359.
Sandery MJ, Forster JW, Blunden R, Jones RN (1990) Identi-fication of a family of repeated sequences on the rye B chromosome. Genome 33: 908–913.
Tsujimoto H, Niwa K (1992) DNA-structure of the B-chromosome of rye revealed by in situ hybridisation using repetitive sequences. Jpn J Genet 67: 233–241.
Vershinin A, Schwarzacher T, Heslop-Harrison JS (1995) The large-scale genomic organisation of repetitive DNA families at the telomeres of rye chromosomes. Plant Cell 7: 1823–1833.
Wilkes TM, Francki MG, Langridge P, Karp A, Jones RN, Forster JW (1995) Analysis of rye B-chromosomes using fluorescence in situ hybridisation (FISH). Chromosome Res 3(8): 466–472.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hasterok, R., Jenkins, G., Langdon, T. et al. The Nature and Destiny of Translocated B-Chromosome-Specific Satellite DNA of Rye. Chromosome Res 10, 83–86 (2002). https://doi.org/10.1023/A:1014278429178
Issue Date:
DOI: https://doi.org/10.1023/A:1014278429178