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Meiotic Chromosomes as Templates for Microdissection

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Abstract

As templates for chromosome microdissection, meiotic cells offer several advantages over mitotic cells. The pairing of homologous chromosomes at the metaphse plate of the first meiotic division allows the simultaneous isolation of two copies of the same chromosome, and the sex chromosomes are easy to identify in male meiotic cells. We report on a method for making fluorescence in-situ hybridization (FISH) probes from dissected meiotic chromosomes.

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References

  • Beechey CV (1995) List of chromosome anomalies. Mouse Genome 93: 407–420.

    Google Scholar 

  • Christian AT, Garcia HE, Tucker JD (1999a) PCR in situ followed by microdissection allows whole chromosome painting probes to be made from single microdissected chromosomes. Mam Genome 10: 628–631.

    Google Scholar 

  • Christian AT, Garcia HE, Tucker JD (1999b) Extending the useful life span of DNA probes for £uorescence in situ hybridization. BioTechniques 26: 1069–1072.

    Google Scholar 

  • Engelen JJ, Albrechts JC, Hamers GJ, Geraedts JP (1998) A simple and ef¢cient method for microdissection and microFISH. J Med Genet 35: 265–268.

    Google Scholar 

  • Evans EP, Breckon G, Ford CE (1964) An air-drying method for meiotic preparation from mammalian testis. Cytogenetics 3: 289–294.

    Google Scholar 

  • Guan XY, Meltzer PS, Trent JM (1994) Rapid generation of whole chromosome painting probes (WCPs) by chromosome microdissection. Genomics 22: 101–107.

    Google Scholar 

  • Tease C (1996) Analysis of dual-color fluorescence in situ hybridization of meiotic chromosome segregation in male mice heterozygous for a reciprocal translocation. Chromosome Res 4: 61–68.

    Google Scholar 

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Christian, A.T., Pattee, M.S. & Marchetti, F. Meiotic Chromosomes as Templates for Microdissection. Chromosome Res 10, 45–48 (2002). https://doi.org/10.1023/A:1014266010522

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  • DOI: https://doi.org/10.1023/A:1014266010522

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