Skip to main content
SpringerLink
Log in

Analysis of Putative Interactions Between Potyviral Replication Proteins and Plant Retinoblastoma Proteins

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Sequence comparisons suggest that the RNA-dependent RNA polymerase (NIb) of potyviruses and bymoviruses, as well as the viral polymerase of potexviruses may contain a putative retinoblastoma protein (pRb) binding motif. The possibility that the potyviral NIb may function in the nucleus through interactions with plant pRb-related (RBR) proteins, and the modifications of the cell cycle was investigated by a combination of mutagenesis of the NIb and yeast two-hybrid system (YTHS). Mutation of a highly conserved glutamic acid residue in the putative pRb-binding motif of the NIb had no detectable phenotypic effect on replication of Potato virus A (PVA). Furthermore, the NIb proteins from Potato virus V and PVA failed to interact with maize or tobacco RBR proteins in yeast. Although the conservation of the motif for pRb interaction in plant RNA viruses is intriguing, these proteins from plant RNA viruses appear not to interact with plant RBR proteins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Mayo M.A. and Pringle C.R., Virus taxonomy—1997, J Gen Virol 79, 649-657, 1998.

    PubMed  Google Scholar 

  2. Riechmann J.L., Laín S., and García J.A., J Gen Virol 73, 1-16, 1992.

    PubMed  Google Scholar 

  3. Shukla D.D., Ward C.W., and Brunt A.A., The Potyviridae. CAB International Wallingford, UK, 1994.

    Google Scholar 

  4. Martín M.T. and García J.A., J Gen Virol 72, 785-790, 1991.

    PubMed  Google Scholar 

  5. Restrepo M.A., Freed D.D., and Carrington J.C., Plant Cell 2, 987-998, 1990.

    PubMed  Google Scholar 

  6. Baunoch D.A., Das P., Browning M.E., and Hari V., J Gen Virol 72, 487-492, 1991.

    PubMed  Google Scholar 

  7. Hajimorad M.R., Ding X.S., Flasinski S., Mahajan E., Graff Haldeman-Cahill R., Carrington J.C., and Cassidy B.G., Virology 224, 368-379, 1996.

    PubMed  Google Scholar 

  8. Langenberg W.G. and Zhang L., J Struct Biol 118, 243-247, 1997.

    PubMed  Google Scholar 

  9. Schaad M.C., Haldeman-Cahill R., Cronin S., and Carrington J.C., J Virol 70, 7039-7048, 1996.

    PubMed  Google Scholar 

  10. Oruetxebarria I., Guo D., Merits A., Mäkinen K., Saarma M., and Valkonen J.P.T. Virus Res 73, 103-112, 2001.

    PubMed  Google Scholar 

  11. Li X.H. and Carrington J.C., Proc Natl Acad Sci USA 92, 457-461, 1995.

    PubMed  Google Scholar 

  12. Li X.H., Valdez P., Olvera R.E., and Carrington J.C., J Virol 71, 1598-1607, 1997.

    PubMed  Google Scholar 

  13. Jansen-Dürr P., Trends Genet 12, 270-275, 1996.

    PubMed  Google Scholar 

  14. Teodoro J.G. and Branton P.E., J Virol 71, 1739-1746, 1997.

    PubMed  Google Scholar 

  15. O'Brien V., J Gen Virol 79, 1833-1845, 1998.

    PubMed  Google Scholar 

  16. Gutiérrez C., EMBO J 19, 792-799, 2000.

    PubMed  Google Scholar 

  17. Aronson M.N., Meyer A.D., Györgyey J., Katul L., Vetten H.J., Gronenborn B., and Timchenko T., J Virol 74, 2967-2972, 2000.

    PubMed  Google Scholar 

  18. Durfee T., Feiler H.S., and Gruissem W., Plant Mol Biol 43, 635-642, 2000.

    PubMed  Google Scholar 

  19. White R.J., Trends Biochem Sci 22, 77-80, 1997.

    PubMed  Google Scholar 

  20. Herwig S. and Strauss M., Euro J Biochem 246, 581-601, 1997.

    Google Scholar 

  21. den Boer B.G.W., and Murray J.A.H., Trends Cell Biol 10, 245-250, 2000.

    PubMed  Google Scholar 

  22. Lavia P. and Jansen-Dürr P., Bioessays 21, 221-230, 1999.

    PubMed  Google Scholar 

  23. Wanitchakorn R., Hafner G.J., Harding R.M., and Dale J.L., J Gen Virol 81, 299-306, 2000.

    PubMed  Google Scholar 

  24. Ghosh A.K., Steele R., Meyer K., Ray R., and Ray R.B., J Gen Virol 80, 1179-1183, 1999.

    PubMed  Google Scholar 

  25. Forng R.-Y. and Atreya C.D., J Gen Virol 80, 327-332, 1999.

    PubMed  Google Scholar 

  26. Aranda M. and Maule A., Virology 243, 261-267, 1998.

    PubMed  Google Scholar 

  27. Aranda M.A., Escaler M., Wang D., and Maule A.J., Proc Natl Acad Sci USA 93, 15289-15293, 1996.

    PubMed  Google Scholar 

  28. Koonin E.V., J Gen Virol 72, 2197-2206, 1991.

    PubMed  Google Scholar 

  29. O'Reilly E.K. and Kao C., Virology, 252, 287-303, 1998.

    PubMed  Google Scholar 

  30. Wang X. and Gillam S., Virology, 285, 322-331, 2001.

    PubMed  Google Scholar 

  31. Andrejeva J., Puurand Ü., Merits A., Rabenstein F., Järvekülg L., and Valkonen J.P.T., J Gen Virol 80, 1133-1139, 1999.

    PubMed  Google Scholar 

  32. Sambrook J., Fritsch E.F., and Maniatis T., Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory Press, New York, 1989.

    Google Scholar 

  33. Hämäläinen J.H., Kekarainen T., Gebhardt C., Watanabe K.N., and Valkonen J.P.T., Mol Plant Microbe Interact 13, 402-412, 2000.

    PubMed  Google Scholar 

  34. Nolasco G., de Blas C., Torres V., and Ponz F., J Virol Methods 45, 201-218, 1993.

    PubMed  Google Scholar 

  35. Xie Q., Sanz-Burgos A.P., Hannon G.J., and Gutiérrez C., EMBO J 15, 4900-4908, 1996.

    PubMed  Google Scholar 

  36. Nakagami H., Sekine M., Murakami H., and Shinmyo A., Plant J 18, 243-252, 1999.

    PubMed  Google Scholar 

  37. Kvarnheden A., Yao J.-L., Zhan X., O'Brien I., and Morris B.A.M., J Exp Bot 51, 1789-1797, 2000.

    PubMed  Google Scholar 

  38. Guo D., Rajamäki M.L., Saarma M., and Valkonen J.P.T., J Gen Virol 82, 935-939, 2001.

    PubMed  Google Scholar 

  39. Gietz D., St Jean A., Woods R.A., and Schiestl R.H., Nucl Acids Res 20, 1425, 1992.

    PubMed  Google Scholar 

  40. Seron K., Tieaho V., Prescianotto-Baschong C., Aust T., Blondel M.O., Guillaud P., Devilliers G., Rossanese O.W., Glick B.S., Riezman H., Keranen S., and Haguenauer-Tsapis R., Mol Biol Cell 9, 2873-2889, 1998.

    PubMed  Google Scholar 

  41. Xie Q., Suárez-López P., and Gutiérrez C., EMBO J 14, 4073-4082, 1995.

    PubMed  Google Scholar 

  42. Liu L., Saunders K., Thomas C.L., Davies J.W., and Stanley J., Virology 256, 270-279, 1999.

    PubMed  Google Scholar 

  43. Nagar S., Pedersen T.J., Carrick K.M., Hanley-Bowdoin L., and Robertson D., Plant Cell 7, 705-719, 1995.

    PubMed  Google Scholar 

  44. Puurand Ü., Mäkinen K., Paulin L., and Saarma M., J Gen Virol 75, 457-461, 1994.

    PubMed  Google Scholar 

  45. Oruetxebarria I., Kekarainen T., Spetz C., and Valkonen J.P.T., Phytopathology 90, 437-444, 2000.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Biology, Genetics Centre, SLU, PO Box 7080, S-750 07, Uppsala, Sweden

    IGOR Oruetxebarria, Anders Kvarnheden & Jari P. T. Valkonen

Authors
  1. IGOR Oruetxebarria
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anders Kvarnheden
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jari P. T. Valkonen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jari P. T. Valkonen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Oruetxebarria, I., Kvarnheden, A. & Valkonen, J.P.T. Analysis of Putative Interactions Between Potyviral Replication Proteins and Plant Retinoblastoma Proteins. Virus Genes 24, 65–75 (2002). https://doi.org/10.1023/A:1014090105049

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1014090105049

Search

Navigation