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Detection of Squalene in Alpha-Fetoprotein and Fetal Serum Albumin from Bovine

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Abstract

Alpha-fetoprotein and fetal serum albumin have been simultaneously purified from fetal bovine serum by mild procedures utilizing ammonium sulfate, hydrophobic interaction, immobilized metal (nickel) affinity chromatography, and isoelectric focusing. The lipidic extract from each protein was analyzed by gas chromatography and the peak appearing just after the arachidonic acid was identified as squalene by gas chromatography-mass spectrometry. This isoprenoid was not detected formerly in these proteins from human, rat, bovine, and pig. Until recently, in the analysis of the fatty acid composition of the alpha-fetoprotein and serum albumin from mammals, a peak has been assigned in the last part of the chromatographic profile, after arachidonic acid, to docosahexaenoic acid. In the present work, it was found that the peak corresponds to squalene instead of docosahexaenoic acid. Furthermore, we conclude that bovine alpha-fetoprotein and fetal serum albumin carry squalene, but not docosahexaenoic acid. These results agree with others obtained analyzing the same proteins from chick embryo.

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Authors and Affiliations

  1. Consejo Superior de Investigaciones Cientificas, Instituto de la Grasa, 41012, Seville, Spain

    V. Ruiz-Gutiérrez & W. Moreda

  2. Laboratorio de Fetoproteinas, Unidad de Virología y Biología del Desarrollo, Facultad de Biología, Campus de Rabanales, Universidad de Córdoba, 14071, Cordoba, Spain

    R. Moreno, M. A. Copado & A. Rodríguez-Burgos

Authors
  1. V. Ruiz-Gutiérrez
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  2. R. Moreno
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  3. W. Moreda
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  4. M. A. Copado
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  5. A. Rodríguez-Burgos
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Ruiz-Gutiérrez, V., Moreno, R., Moreda, W. et al. Detection of Squalene in Alpha-Fetoprotein and Fetal Serum Albumin from Bovine. J Protein Chem 20, 19–23 (2001). https://doi.org/10.1023/A:1011096702910

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  • DOI: https://doi.org/10.1023/A:1011096702910

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