Abstract
Amyloglucosidase EC 3.2.1.3 was used as model for the immobilisation of enzymes in poly(vinylalcohol) hydrogel (LentiKats) lenses. The entrapment of the enzyme in PVA-hydrogel based on a two-step procedure, firstly its coupling to polyelectrolytes increased the structure level of the enzyme, and subsequently, the resulting complex was entrapped in LentiKats. The immobilised enzyme retained 45% of its original activity and lost no activity over five repeated batch runs.
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Czichocki, G., Dautzenberg, H., Capan, E. et al. New and effective entrapment of polyelectrolyte-enzyme-complexes in LentiKats. Biotechnology Letters 23, 1303–1307 (2001). https://doi.org/10.1023/A:1010569322537
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DOI: https://doi.org/10.1023/A:1010569322537