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The Influence of Hyperbaric Oxygenation (HBO) on Proliferation and Differentiation of Human Keratinocyte Cultures In Vitro

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Abstract

A drop in tissue oxygen partial pressure below 30 mm Hg as a result of reduced perfusion in an extensive area of acute skin damage, or where a large number of chronic skin defects occur, inhibits collagen synthesis and neoangiogenesis in the various phases of wound healing. Subsequent granulation and epithelialisation are correspondingly impaired.

Hyperbaric oxygenation is now recognised as a valuable supplementary method of treatment for problematic wounds. Stimulation of fibroblast and endothelial cell proliferation through Hyperbaric oxygenation has been demonstrated in numerous studies.

The aim of this study was to investigate the effect of hyperbaric oxygen treatment on the proliferation and differentiation of human keratinocyte cultures.

The influence of hyperbaric oxygenation on the proliferation of human keratinocyte cultures was demonstrated using flow-through cytometry and a fluorescence activated cell sorter, which detects fluorescence intensity following incorporation of 5-bromo-2′-deoxyuridine in cell DNA.

The degree of cell differentiation was deduced from the expression of various components of the cytoskeleton, such as cytokeratin 10 and involukrin, the production of which was quantified through the determination of monoclonal antibodies against cytokeratin 10 and involukrin from measurements of fluorescence activity in a flow-through cytometer.

Hyperbaric oxygenation of cell cultures in vitro did not produce a significantly higher rate of cell proliferation, so that no increase in vitality was observed.

An interesting observation following exposure to hyperbaric oxygen was the marked increase in expression of both cytokeratin 10 and involukrin, as an indication of accelerated cell differentiation.

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Authors and Affiliations

  1. Department of Trauma and Reconstructive Surgery, Johann Wolfgang Goethe University Clinics, Theodor Stern-Kai 7, 60590, Frankfurt/Main, Germany

    Dirk A. Hollander

  2. Department of Trauma and Reconstructive Surgery, Johann Wolfgang Goethe University Clinics, Theodor Stern-Kai 7, 60590, Frankfurt/Main, Germany

    Mohssen Y. Hakimi, Andre Hartmann, Kerstin Wilhelm & Joachim Windolf

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  1. Dirk A. Hollander
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  5. Joachim Windolf
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Hollander, D.A., Hakimi, M.Y., Hartmann, A. et al. The Influence of Hyperbaric Oxygenation (HBO) on Proliferation and Differentiation of Human Keratinocyte Cultures In Vitro. Cell Tissue Banking 1, 261–269 (2000). https://doi.org/10.1023/A:1010145312698

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