Abstract
A three-dimensional cell coculture method is presented where cancer cells can be maintained alone or combined with other cell types in longterm culture in order to reconstitute some of the interactions between the different cell elements in tumors in vivo. The cells are accumulated by centrifugation to form ‘nodules’ which are cultivated on a semi-solid agar medium at medium/air interface. The nodules are not mere cell aggregates, they are able to develop morphological and functional differentiation as well as tissue-like membrane junctions. Studies on short-term and long-term effects of anticancer treatments are possible and their long-term regrowth can be obtained. Especially, in nodules containing cell mixtures, the localization of the different cell types can be determined and their specific differentiation. An example showing stroma-like formations and collagen production in breast cancer cell and breast fibroblast containing nodules is presented.
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References
Beaupain R, Baroche C, Lagarde D (1983). Long term regeneration of cis-platinum and X-ray treated human tumor nodules maintained in continuous organotypic culture. Int J Radiation Oncology Biol Phys 9: 707–712.
Beaupain R, Billard C, Falcoff E (1986). Effects of human recombinant interferons-α2, -β and -γ on growth and survival of human cancer nodules maintained in continuous organotypic culture. Eur J Cancer Clin Oncol 22: 141–149.
Beaupain R, Dionet C (1985). Effects of combined treatments of cis-diamminedichloroplatinum, 5-fluorouracil and X-rays on growth of human cancer nodules maintained in continuous organotypic culture. Cancer Res 45: 3150–3154.
Beaupain R, Martyré MC (1990). Short-and longterm synergistic effects of human tumor necrosis factor and interferon-γ on A549 human lung cancer cells maintained in three-dimensional culture. Anticancer Res 10: 1061–1066.
Beaupain R, Prévost G, Mainguené C, Lainé-Bidron C, Tamboise A, Tamboise E (1992). Continuous threedimensional culture of MCF-7 cells in serum free medium. In Vitro Cell Dev Biol 29A: 893–898.
Brouty-Boyé D, Mainguené C, Magnien V, Israel L, Beaupain R (1994). Fibroblast-mediated differentiation in human breast carcinoma cells (MCF-7) grown as nodules in vitro. Int J Cancer 56: 731–735.
Lyser K (1976). Adaptations of the culture method of Wolff for neoplastic nervous system. Tissue Culture Association Procedure 41177: 133–138.
Martyré MC, Beaupain R, Falcoff E (1987). Potentiation of antiproliferative activity by mixtures of human recombinatnt IFN-α2 and -γ on growth of human cancer nodules maintained in continuous organotypic culture. Eur J Cancer Clin Oncol 23: 917–922.
Rofstad EK (1991). Spheroids and xenografts. In: Masters JRW (ed), Human cancer in primary culture, a hand book, pp 81–101. The Netherlands: Kluwer Academic Publishers.
Sutherland RM (1988). Cell and environment interactions in tumor microregions: The multicell spheroid model. Science 240: 177–184.
Wolff Et, Wolff Em (1975). Current research with organ cultures of human tumors. In: Fogh J (ed), Human Tumor Cells in Vitro, pp 207–240. London/New York: Plenum Press.
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Beaupain, R. A method for three-dimensional coculture of cancer cells combined to any other type of cells maintained organotypically. Methods Cell Sci 21, 25–30 (1999). https://doi.org/10.1023/A:1009899527587
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DOI: https://doi.org/10.1023/A:1009899527587