Skip to main content
SpringerLink
Log in

Sequence of DNA Replication in Allium Fistulosum Chromosomes During S-phase

  • Published:
Chromosome Research Aims and scope Submit manuscript

Abstract

Bromodeoxyuridine pulse labelling and immunodetection were applied to synchronized Allium fistulosum cells to study sequential changes in the chromosome replication pattern during S-phase. The replication patterns were classified into five main types depending on the location of the replication signals: (1) over the whole chromosomes; (2) at proximal and interstitial regions; (3) at proximal, interstitial and distal regions; (4) at interstitial and distal regions; and (5) at distal regions. The frequencies of each pattern changed sequentially according to the timing of BrdU incorporation, demonstrating the temporal order of chromosome replication during S-phase. The distal regions that correspond to the major C-bands replicated throughout S-phase except for the earliest stage, but most intensely in late S-phase. The replication time of different chromosome sites overlapped, which is quite different from the biphasic mode of replication tha t occurs in mammalian chromosomes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barnes SR, James AM, Jamieson G (1985) The organization, nucleotide sequence, and chromosomal distribution of a satellite DNA from Allium cepa. Chromosoma 92: 185–192.

    Google Scholar 

  • Cortés F, Escalza P (1986) Analysis of different banding patterns and late replicating regions in chromosomes of Allium cepa, A. sativum and A. nigrum. Genetica 71: 39–46.

    Google Scholar 

  • Cortés F, Gonzalez-Gil G, López-Saez JF (1980) Differential staining of late replicating DNA-rich regions in Allium cepa chromosomes. Caryologia 33: 193–202.

    Google Scholar 

  • Cortés F, Escalza P, Sosa MD, López-Campos JL (1983) Cytological demonstration of DNA synthesis inhibition at late S by 5-amino-uracil. Exp Cell Res 148: 503–507.

    Google Scholar 

  • Darlington CD, Haque A (1966) Organisation of DNA synthesis in rye chromosomes. Chrom Today 1: 102–107.

    Google Scholar 

  • Döbel P, Schubert I, Rieger R (1978) Distribution of heterochromatin in a reconstructed karyotype of Vicia faba as identified by banding-and DNA-late replication patterns. Chromosoma 69: 193–209.

    Google Scholar 

  • Dutrillaux B (1975) Obtention simultanée de plusiers marquages chromosomiques sur les mêmes préparations, aprés traitement par le BrdU. Humangenetik 30: 297–306.

    Google Scholar 

  • El-Gadi A, Elkington, TT (1975) Comparison of the Giemsa C-band karyotypes and the relationships of Allium cepa, A. fistulosum and A. galanthum. Chromosoma 51: 9–23.

    Google Scholar 

  • Epplen JT, Siebers J-W, Vogel W (1975) DNA replication patterns of human chromosomes from fibroblasts and amniotic fluid cells revealed by a Giemsa staining technique. Cytogenet Cell Genet 15: 177–185.

    Google Scholar 

  • Fiskesjö G (1974) Two types of constitutive heterochromatin made visible in Allium by a rapid C-banding method. Hereditas 78: 153–156.

    Google Scholar 

  • Fussell CP (1975) The position of interphase chromosomes and late replicating DNA in centromere and telomere regions of Allium cepa L. Chromosoma 50: 201–210.

    Google Scholar 

  • Holmquist G, Gray M, Porter T, Jordan J (1982) Characterization of Giemsa dark-and light-band DNA. Cell 31: 121–129.

    Google Scholar 

  • Irifune K, Hirai K, Zheng J, Tanaka R, Morikawa H (1995) Nucleotide sequence of a highly repeated DNA sequence and its chromosomal localization in Allium fistulosum. Theor Appl Genet 90: 312–316.

    Google Scholar 

  • Kakeda K, Yamagata H (1992) Immunological analysis of chromosome replication in barley, rye, and durum wheat by using an anti-BrdU antibody. Hereditas 116: 67–70.

    Google Scholar 

  • Klevecz RR, Keniston BA, Deacen LL (1975) The temporal structure of S-phase. Cell 5: 195–203.

    Google Scholar 

  • Kuroiwa T, Tanaka N (1970) DNA replication pattern in somatic chromosomes of Crepis capillaris. Cytologia 35: 271–279.

    Google Scholar 

  • Latt SA (1973) Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc Natl Acad Sci USA 70: 3395–3399.

    Google Scholar 

  • Latt SA (1975) Fluorescence analysis of late DNA replication in human metaphase chromosomes. Somat Cell Genet 1: 293–321.

    Google Scholar 

  • Maggini F, Barsanti P, Marazia T (1978) Individual variation of the nucleolus organizer regions in Allium cepa and A. sativum. Chromosoma 66: 173–183.

    Google Scholar 

  • Pich U, Fuchs J, Schubert I (1996) How do Alliaceae stabilize their chromosome ends in the absence of TTTAGGG sequences? Chrom Res 4: 207–213.

    Google Scholar 

  • Richer C-L, Drouin R (1990) Dynamic banding for high-resolution analysis of chromosomes and assignment of DNA replication times. In: Obe G, ed. Advances in Mutagenesis Research 2, pp 55–94. Berlin: Springer-Verlag.

    Google Scholar 

  • Sato S (1981) Cytological studies on the satellited chromosomes of Allium cepa. Caryologia 34: 431–440.

    Google Scholar 

  • Schmidt M (1980) Two phases of DNA replication in human cells. Chromosoma 76: 101–110.

    Google Scholar 

  • Schubert I, Rieger R (1979) Asymmetric banding of Vicia faba chromosomes after BrdU incorporation. Chromosoma 70: 385–391.

    Google Scholar 

  • Schweizer D, Strehl S, Hagemann S (1990) Plant repetitive DNA elements and chromosome structure. Chrom Today 10: 33–43.

    Google Scholar 

  • Sumner AT (1990) Chromosome Banding. London: Unwin Hyman.

    Google Scholar 

  • Tanaka R, Taniguchi K (1975) A banding method for plant chromosomes. Jpn J Genet 50: 163–167.

    Google Scholar 

  • Taniguchi K, Fujishige I (1996) Replication of chromosomes. In: Fukui K, Nakayama S, eds. Plant Chromosomes: Laboratory Methods. Boca Raton: CRC Press.

    Google Scholar 

  • Taniguchi K, Tanaka R (1991) Visualization of replicating bands in plant chromosomes with a monoclonal anti-BrdU antibody method. Jpn J Genet 66: 485–489.

    Google Scholar 

  • Taylor JH (1958) The mode of chromosome duplication in Crepis capillaris. Exp Cell Res 15: 350–357.

    Google Scholar 

  • Uozu S, Nakazaki T, Tanisaka T (1997) Characterization of the late replication banding patterns in barley chromosomes. Genes Genet Syst 72: 125–130.

    Google Scholar 

  • Vosa CG (1976a) Heterochromatic patterns in Allium. I. The relationship between the species of the cepa group and its allies. Heredity 36: 383–392.

    Google Scholar 

  • Vosa CG (1976b) Heterochromatic banding patterns in Allium. II. Heterochromatin variation in species of the paniculatum group. Chromosoma 57: 119–133.

    Google Scholar 

  • Wimber DE (1961) Asynchronous replication of deoxyribonucleic acid in root tip chromosomes of Tradescantia paludosa. Exp Cell Res 23: 402–407.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Plant Chromosome and Gene Stock, Faculty of Science, Hiroshima University, Higashi-Hiroshima, 739, Japan

    I. Fujishige & K. Taniguchi

Authors
  1. I. Fujishige
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Taniguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fujishige, I., Taniguchi, K. Sequence of DNA Replication in Allium Fistulosum Chromosomes During S-phase. Chromosome Res 6, 611–619 (1998). https://doi.org/10.1023/A:1009201409644

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1009201409644

Search

Navigation