Abstract
Surface modification techniques were employed in order to provide functionalized silicone rubber with enhanced cytocompatibility. Acrylic acid (AAc), methacrylic acid (MAAc) and glycidylmethacrylate (GMA) were graft-co-polymerized onto the surface of silicone induced by an argon plasma and thermal initiation. The polymerizations were carried out in solution, in the case of acrylic acid a vapor phase graft-co-polymerization subsequent to argon plasma activation was carried out as well. Human fibronectin (hFn), which acts as a cell adhesion mediator for fibroblasts, was immobilized by making use of the generated carboxylic or epoxy groups, respectively. Surface analysis was accomplished by means of X-ray photoelectron spectroscopy (XPS), infrared spectroscopy in attenuated total reflection mode (IR-ATR), scanning electron microscopy (SEM), atomic force microscopy (AFM) and dynamic contact angle measurements using the Wilhelmy-plate method. The amount of immobilized active hFn was semiquantified by enzyme-linked immunosorbent assay (ELISA) using a structure-specific antibody against the cell-binding domain of hFn. In vitro testing showed a remarkable difference between surfaces exposing adsorbed-only and surfaces with covalently immobilized hFn. © 2001 Kluwer Academic Publishers
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
B. ARKLES, CHEMTECH 13 (1983) 542.
M. REIM, Ophthalmology 89 (1992) 109.
L. REINBACHER, Ärztlicher Praxis 96 (1995) 5.
S. A. VISSER, R. W. HERGENROTHER and S. L. COOPER in ``Biomaterials Science: An Introduction to Materials in Medicine'', edited by B. D. Ratner, A. S. Hoffman, F. J. Schoen and J. E. Lemons (Academic Press, New York, 1998) p. 59.
S. S. BACON, C. ASTIN and J. DART, Cornea 13 (1994) 422.
G. KOERNER, M. SCHULZE and J. WEIS (editors) in ``Silicones: Chemistry and Technology'' (Vullkan Publishing, Essen, 1991).
S.-H. CHANG, US Patent, WO 91/04288, 1991.
H.-J. HETTLICH, F. OTTERBACH and C. MITTERMAYER, Biomaterials 12 (1991) 521.
T. OKADA and Y. IKADA, J. Biomed. Mater. Res. 26 (1992) 1569.
G. H. HSIUE, S.-D. LEE, C.-C. WANG and P. CHANG, J. Biomater. Sci. Polymer Edn. 5 (1993) 205.
A. S. HOFFMAN in ``Biomaterials Science: An Introduction to Materials in Medicine'', edited by B. D. Ratner, A. S. Hoffman, F. J. Schoen and J. E. Lemons (AcademicPress, NewYork, 1998),p.128.
G.-H. HSIUE, S.-D. LEE, C.-C. WANG, M. HSIUE and P. CHANG, Biomaterials 15 (1994) 163.
T. A. HORBETT, in ``Protein Adsorption to Hydrogels'' (Vol. 1, CRC Press, 1986).
S. SANO, K. KATO and Y. IKADA, Biomaterials 14 (1993) 817.
L. J. GOGUÉ, N. MERMILLIOD and A. GANDINI, J. Appl. Polym. Sci. 56 (1995) 33.
X. COQUERET, A. LABLACHE-COMBIER and C. LOUCHEUX, Eur. Polym. J. 24 (1988) 1137.
D. F. MOSHER, Ann. Rev. Med. 35 (1984) 561.
J. G. STEELE, B. A. DALTON, G. JOHNSON and P. A. UNDERWOOD, Biomaterials 16 (1995) 1057.
A. S. HOFFMAN, Artificial Organs 16 (1992) 43.
J. M. LEE, H. H. L. EDWARDS, C. A. PEREIRA and S. I. SAMII, J. Mater. Sci.: Mater. Med. 7 (1996) 531.
C. J. CLIFFORD and S.DOWNES, ibid., 7 (1996) 637.
J. G. A. TERLINGEN, H. F. C. GERRITSEN, A. S. HOFFMAN and J. FEIJEN, J. Appl. Polym. Sci. 57 (1995) 969.
B. RATNER and D. G. CASTNER, Coll. Surf. B: Biointerfaces 2 (1994) 333.
M. J. OWEN, T. M. GENTLE, T. ORBECK and D. E. WILLIAMSIN, in “Polymer Surface Dynamics”, edited by J. Andrade (Plenum Press, New York, 1988), p. 101.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vo¨lcker, N., Klee, D., Ho¨cker, H. et al. Functionalization of silicone rubber for the covalent immobilization of fibronectin. Journal of Materials Science: Materials in Medicine 12, 111–119 (2001). https://doi.org/10.1023/A:1008938525489
Issue Date:
DOI: https://doi.org/10.1023/A:1008938525489