Skip to main content
SpringerLink
Log in

Comparison of the Efficiency of Moloney Murine Leukaemia Virus (M-MuLV) Reverse Transcriptase, RNase H--M-MuLV Reverse Transcriptase and Avian Myeloblastoma Leukaemia Virus (AMV) Reverse Transcriptase for the Amplification of Human Immunoglobulin Genes

  • Published:
Biotechnology Techniques

Abstract

The efficiency of three commonly used reverse transcriptases, namely Moloney Murine Leukaemia Virus (M-MuLV) reverse transcriptase, RNase H--M-MuLV reverse transcriptase and Avian Myeloblastoma Leukaemia Virus (AMV) reverse transcriptase (RT), have been compared in RT-PCR amplification of antibody genes. These enzymes differed significantly in their efficiency of cDNA synthesis as indicated by the degree of V gene amplification by PCR; Moloney Murine Leukaemia Virus (M-MuLV) reverse transcriptase and RNase H--M-MuLV reverse transcriptase produced greater amplification of the human V-gene repertoire using RT-PCR than that of the Avian Myeloblastoma Leukaemia Virus (AMV) reverse transcriptase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bøyum, A. (1968). Scand. J. Clin. Lab. Invest. 21:Suppl. 97:77-89.

    Google Scholar 

  • Kabat, E.A., Wu, T.T., Perry, H.M., Gottesman, K.S. and Foeller, C. (1991) Sequences of Proteins of Immunological Interest. 5th edn. U.S. Department of Health and Human Services, Public Health Service National Institutes of Health.

  • Leinhard, G.E., Secemski, I.I., Koehler, K.A. and Lindquist, R.M. (1971) Gold Spring Harbor Symp. Quant. Biol. 36:45-51.

    Google Scholar 

  • Marks, J.D., Hoogenboom, H.R., Bonnert, T., McCafferty, J., Griffiths, A.D. and Winter, G. (1991) J. Mol. Biol. 222:581-597.

    Google Scholar 

  • Marks, J.D., Tristem, M., Karpas, A., and Winter, G. (1991) Eur. J. Immumol. 21: 985-991.

    Google Scholar 

  • Orlandi, R., Güssow, D.H., Jones, P.T. and Winter, G. (1989) Proc. Natl. Acad. Sci. USA. 86: 3833-3837.

    Google Scholar 

  • Persson, M.A.A., Caothien, R.H. and Burton, D.R. (1991) Proc. Natl. Acad. Sci. U.S.A. 88: 2432-2436.

    Google Scholar 

  • Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A laboratory Manual, Volume 1, Section 7.6, John Wiley & Sons, Plainview, NY.

    Google Scholar 

  • Tomlinson, I.M., Walter, G., Marks, J.D., Llewelyn, M.B. and Winter, G. (1992) J. Mol. Biol. 227: 776-798.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, University of New South Wales, NSW, 2052, Australia

    Louisa Wong, Christopher Peter Marquis & Stephen Mahler

  2. Australian Red Cross Blood Service, 151 Clarence St., Sydney, NSW, 2000, Australia

    Helen Pearson & Anne Fletcher

Authors
  1. Louisa Wong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Helen Pearson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Anne Fletcher
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Christopher Peter Marquis
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Stephen Mahler
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wong, L., Pearson, H., Fletcher, A. et al. Comparison of the Efficiency of Moloney Murine Leukaemia Virus (M-MuLV) Reverse Transcriptase, RNase H--M-MuLV Reverse Transcriptase and Avian Myeloblastoma Leukaemia Virus (AMV) Reverse Transcriptase for the Amplification of Human Immunoglobulin Genes. Biotechnology Techniques 12, 485–489 (1998). https://doi.org/10.1023/A:1008871608947

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1008871608947

Keywords

Search

Navigation