Abstract
Prawn waste, a chitinous solid waste of the shellfish processing industry, was used as a substrate for chitinase production by the marine fungus Beauveria bassiana BTMF S10, in a solid state fermentation (SSF) culture. The␣process parameters influencing SSF were optimized. A maximum chitinase yield of 248.0 units/g initial dry substrate (U/gIDS) was obtained in a medium containing a 5:1 ratio (w/v) of prawn waste/sea water, 1% (w/w) NaCl,␣2.5% (w/w) KH2PO4, 425–600μm substrate particle size at 27°C, initial pH 9.5, and after 5 days of incubation. The presence of yeast extract reduced chitinase yield. The results indicate scope for the utilization of shellfish processing (prawn) waste for the industrial production of chitinase by using solid state fermentation.
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Aidoo, K.E., Hendry, R. & Wood, B.J.B. 1982 Solid State Fermentations. Advances in Applied Microbiology 28, 201–237.
Ashford, N.A., Hattis, D. & Murray, A.E. 1977 Industrial prospects for chitin and protein from shellfish wastes. M.I.T. Sea Grant Program report number MITSG-77-3 index number 77-703-Zle. Massachusetts: Massachusetts Institute of Technology.
Balakrishnan, K. & Pandey, A. 1996 Production of biologically active secondary materials in solid state fermentation. Journal of Scientific and Industrial Research 55, 365–372.
Carroad, A.P. & Tom, R.A. 1978 Bioconversion of shellfish chitin wastes: process conception and selection of microorganisms. Journal of Food Science 43, 1158–1165.
Casio, I.G., Fisher, R.A. & Carroad, P.A. 1982 Bioconversion of shellfish chitin waste: waste pre-treatment, enzyme production, process design and economic analysis. Journal of Food Science 47, 901–911.
Chandrasekaran, M. 1994 Economic utilization of marine microorganisms employing solid state fermentation. In Solid State Fermentation, ed Pandey, A. pp. 168–172. New Delhi: Wiley Eastern Limited.
Chandrasekaran, M. 1996 Harnessing marine micro-organisms through solid state fermentation. Journal of Scientific and Industrial Research 55, 468–471.
Chandrasekaran, M., Lakshmanapermalsamy, P. & Chandramohan, D. 1991 Combined effect of environmental factors on spoilage bacteria. Fishery Technology (India) 28, 146–153.
Gernot, V. 1983 Actinoplanetes. In Bergey's Manual of Systematic Bacteriology, Vol. 4, eds Williams, S.T., Sharpe, M.E. & Holt, J.G. pp. 2418–2450. Baltimore: Williams & Wilkins.
Ghidyal, N.P., Lonsane, B.K., Sreekantiah, K.R. & Murthy, V.S. 1985 Economics of submerged and solid state fermentations for the production of amyloglucosidase. Journal of Food Science and Technology 22, 171–176.
Healy, M.G., Romo, C.R. & Bustos, R. 1994 Bioconversion of marine crustacean shell waste. Resource, Conservation and Recycling 11, 139–147.
Hedges, A. & Wolf, R.S. 1974 Extracellular enzyme from Myxobactor AL-1 that exhibits both β-1, 4-glucanase and chitosanase activities. Journal of Bacteriology 120, 844–847.
Huang, S.Y., Waug, H.H., Wei, C-J., Malaney, G.W. & Tanner, R.D. 1985 Kinetic responses of the koji solid state fermentation processes. In Topics in Enzyme and Fermentation Biotechnology Vol. 10, ed Wiseman, A. pp. 88–108. Chichester: Ellis Horwood.
Jones, E.B.G. & Byrne, P.J. 1976 Physiology of the higher marine fungi. In Recent Advances in Aquatic Mycology, ed Jones, E.B.G. pp. 135–175. London: Paul Elek (Scientific Books) Limited.
Jones, E.B.G. & Irvine, J. 1972 The role of marine fungi in the biodeterioration of materials. In Biodeterioration of Materials, eds Walters, A.H. & Huck-Van der Plus, E.H. pp. 422–431. London: Applied Science.
Lonsane, B.K., Ghildyal, N.P., Budiatman, S. & Ramakrishna, S.V. 1985 Engineering aspects of solid state fermentation. Enzyme and Microbial Technology 7, 258–265.
Madhavan, P. & Nair, K.G.R. 1975 Chitosan from Squilla. Fishery Technology (India) 7, 81–82.
Madhavan, P., Nair, K.G.R., Thankappan, T.K. Prabhu, P.V. & Gopakumar, K. 1986 Production of Chitin and Chitosan, Kochi, India: Central Institute of Fisheries Technology.
Miller, G.L. 1959 Use of dinitrosalicylic acid for estimation of reducing sugar. Analytical Chemistry 31, 426–428.
Molitoris, H.P. & Schaumann, K. 1986 Physiology of marine fungi: a screening programme for growth and enzyme production. In Biology of Marine Fungi, ed Moss, S.T. pp. 35–47. Cambridge: Cambridge University Press.
Moriguchi, M., Sakai, K., Takyana, Y. & Wakayana, M. 1994 Isolation and characterization of salt tolerant glutaminase from marine Micococcus luteus K-3. Journal of Fermentation and Bioengineering 77, 621–625.
Muniswaran, P.K.A & Charyulu, N.C.L.N. 1994 Solid substrate fermentation of coconut coir pith for cellulase production. Enzyme and Microbial Technology 16, 436–440.
Nagendra, P.G. & Chandrasekaran M. 1996 L-glutaminase production by marine Vibrio costicola under solid state fermentation using different substrates. Journal of Marine Biotechnology 4, 176–179
Nirmala, R.-R. 1991 Shrimp Waste Utilzation, INFOFISH Technical Handbook 4. Kulalumpur: INFOFISH.
Nishio, N., Tai, K. & Nagai, S. 1979 Hydrolase production by Aspergillus niger in solid state cultivation. European Journal of Applied Microbiology and Biotechnology 8, 263–270.
Pegg, G.F. 1988 Chitinase from Verticillium albo-atrum. In Methods in Enzymology, Vol. 161, ed Sabobo, G.D. pp. 474–479. London: Academic Press.
Raimbault, M. & Alezard, D. 1980 Culture method to study fungal growth in solid state fermentation. European Journal of Applied Microbiology 9, 199–209.
Ramesh, M.V. & Lonsane, B.K. 1990 Critical importance of moisture content of the medium in alpha amylase production by Bacillus licheniformis M 27 in a solid state fermentation system. Applied Microbiology and Biotechnology 33, 501–505.
Revah-Moiseev, S. & Carroad, P.A. 1981 Conversion of the enzymatic hydrolysate of shellfish waste chitin to single cell protein. Biotechnology and Bioengineering 23, 1067–1073.
Sandhya, X. & Lonsane, B.K. 1994 Factors influencing fungal degradation of total soluble carbohydrates in sugar cane pressmud under solid state fermentation. Process Biochemistry 29, 259–301.
Smith, J.E. & Aidoo, K.E. 1988 Growth of fungi on solid substrate. In Physiology of Industrial Fungi, ed Berry, D.R. pp. 249–269. Oxford: Blackwell Scientific Publications.
Soni, S.K., Bath, K.S. & Soni, R. 1996 Production of amylase by Saccharomycopsis capsularis in solid state fermentation. Indian Journal of Microbiology 36, 157–159.
Suresh, P.V. 1996 Chitinase production by marine fungi. PhD thesis, Cochin University of Science and Technology, Kochi, India.
Tengerdy, R.P. 1992 Solid state fermentation of lignocellulose. In Solid State Cultivation, eds Doelle, H.W., Mitchell, D.A. & Rolz, C.E. pp. 269–282. London: Elsevier Applied Science.
Vyas, P.R. & Deshpande, M.V. 1991 Enzymatic hydrolysis of chitin by Myrothecium verrucaria chitinase complex and its utilization to produce SCP. Journal of General and Applied Microbiology 37, 267–275.
Yang, S.S. 1988 Protein enrichment of sweet potato residue with amylolytic yeast by solid state fermentation. Biotechnology and Bioengineering 32, 886–890.
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Suresh, P., Chandrasekaran, M. Utilization of prawn waste for chitinase production by the marine fungus Beauveria bassiana by solid state fermentation. World Journal of Microbiology and Biotechnology 14, 655–660 (1998). https://doi.org/10.1023/A:1008844516915
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DOI: https://doi.org/10.1023/A:1008844516915