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Lipoxygenase Metabolites of α-linolenic Acid in the Development of Resistance in Pigeonpea, Cajanus cajan (L.) Millsp, Seedlings Against Fusarium udum Infection

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Abstract

Lipoxygenase (LOX) activity was measured in germinating pigeonpea Cajanus cajan seedlings, resistant (ICP-8863) and susceptible (ICP-2376) to wilt fungus, before and after infection with Fusarium udum. LOX activity was significantly higher in the resistant than in the susceptible cultivars of pigeonpea and was enhanced further in response to infection with Fusarium udum. This increase in LOX activity in the resistant cultivars of pigeonpea appears to be due to the induction of lipoxygenase isozymes in response to infection. Analysis of the endogenous LOX metabolites in pigeonpea seedlings revealed the predominant formation of 13-hydroperoxyoctadecadienoic acid (13-HPODE) in healthy seedlings and 13-hydroperoxyoctadecatrienoic acid (13-HPOTrE) in infected seedlings. Further studies on the effects of LOX metabolites on the growth and multiplication of Fusarium udum showed that HPOTrEs, LOX metabolites of α-linolenic acid, are more anti-fungal compared to HPODEs, LOX metabolites of linoleic acid.

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Authors and Affiliations

  1. School of Life Sciences, University of Hyderabad, Hyderabad –, 500 046, India

    P. Uma Maheswari Devi, P. Srinivas Reddy & M. Narsa Reddy

  2. Department of Botany, Osmania University, Hyderabad –, 500 007, India

    N.R. Usha Rani & K.J. Reddy

  3. School of Life Sciences, University of Hyderabad, Hyderabad –, 500 046, India;

    P. Reddanna

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  1. P. Uma Maheswari Devi
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  2. P. Srinivas Reddy
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  3. N.R. Usha Rani
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  4. K.J. Reddy
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  5. M. Narsa Reddy
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  6. P. Reddanna
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Uma Maheswari Devi, P., Srinivas Reddy, P., Usha Rani, N. et al. Lipoxygenase Metabolites of α-linolenic Acid in the Development of Resistance in Pigeonpea, Cajanus cajan (L.) Millsp, Seedlings Against Fusarium udum Infection. European Journal of Plant Pathology 106, 857–865 (2000). https://doi.org/10.1023/A:1008797006206

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