Abstract
We report here the production of transgenic plants containing the P1 gene of the plum pox potyvirus genome. Two P1 constructs were engineered into Nicotiana benthamiana host plants via Agrobacterium tumefaciens. When challenged with PPV, transgenic plant lines were either immune to or recovered from infection. Biochemical studies conducted on these plant lines showed that the resistance phenotypes were correlated with the level of transgene transcript. Plant transformation with the P1 gene is a potentially useful alternative approach to create resistant plants.
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Audy P, Palukaitis P, Stack SA and Zaitlin M (1994) Replicase-mediated resistance to potato virus Yin transgenic tobacco plants. Mol Plant-Microbe Interact 7: 15-22
Baulcombe DC (1996) Mechanism of pathogen-derived resistance to viruses in transgenic plants. The Plant Cell 8: 1833-1844
Brantley JD and Hunt AG (1993) The N-terminal protein of the polyprotein encoded by the tobacco vein mottling virus is an RNA-binding protein. J Gen virol 74: 1157-1162
Candresse T, Macquaire G, Lanneau M, Bousalem M, QuiotDouine L, Quiot JB and Dunez J (1995) Analysis of plum pox virus variability and developement of strain-specific PCR assay. Acta Horticulturae: 357-369
Cassidy BG and Nelson RS (1995) Differences in protection phenotypes in tobacco plants expressing coat protein genes from peanut stripe potyvirus with or without an engineered ATG. Mol Plant-Microbe Interact 8: 357-365
Dosba F, Orliac S, Dutranoy F, Maison P, Massonié G and Audergon JM (1992) Evaluation of resistance to plum pox virus in apricot trees. Acta Horticulturae 309: 211-220
Dougherty WG, Lindbo JA, Smith HA, Parks TD, Swaney S and Proesting WM (1994) RNA-mediated virus resistance in transgenic plants: Exploitation of cellular pathway possibly involved in RNA degradation. Mol Plant-Microbe Interact. 7: 544-552
Flavell RB (1994) Inactivation of gene expression in plants as a consequence of specific sequence duplication. Proc Natl Acad Sci USA 91: 3490-3496
Guo HS and Garcia JA (1997) Delayed resistance to plum pox potyvirus mediated by a mutated RNA replicase gene: involvement of a gene-silencing mechanism. Mol. Plant-Microbe Interact. 10: 160-170
Horsch RB, Fry JE, Holffmann NL, Eicholtz D, Rogers SG and Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227: 179-180
Lindbo JA and Dougherty WG (1992) Untranslatable transcripts of the tobacco etch virus coat gene sequence can interfere with tobacco etch virus replication in transgenic plant and protoplasts. Virology 189: 725-733
Lindbo JA, Silva-Rosales L and Dougherty WG (1993a) Pathogen-derived resistance to potyvirus: Working but why? Seminars in virology 4, 369-379.
Lindbo, JA Silva-Rosales, L, Proesbsting, WM and Dougherty, WG (1993b) Induction of a highly specific antiviral state in transgenic plants: implications for regulation of gene expression and virus resistance. The Plant Cell 4: 1749-1759
Maiti IB, Murphy JF, Shaw JG and Hunt AG (1993) Plants that express a potyvirus proteinase gene are resistant to virus infection. Pro Natl Acad Sci USA 90: 6110-6114
Pehu TM, Maki-Valkama TK, Valkonen KT, Koivu KT, Lehto KM and Pehu EP (1995) Potato plants transformed with a potato virus Y P1 gene sequence are resistant to PVY0. American Potato J. 72: 523-532
Powell-Abel P, Nelson RS, De B, Hoffman N, Rogers SG, Fraley RT and Beachy RN (1986) Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232: 738-743
Ravelonandro M, Monsion M, Delbos R and Dunez J (1993) Variable resistance to plum pox virus and potato virus Y infection in transgenic Nicotianaplants expressing plum pox virus coat protein. Plant Science 91: 157-169
Regner F, da Camara Machado A, Laimer da Camara Machado M, SteinKellner H, Mattanovitch D, Hanzer V and Katinger H (1992) Coat protein mediated resistance to plum pox in Nicotiana clevelandiiand N. benthamiana. Plant Cell Rep. 11: 30-33
Smith HA, Swaney SL, Parks TD, Wernsman EA and Dougherty WG (1994) Transgenic plant virus resistance mediated by untranslatable sense RNAs: Expression, regulation, and fate of nonessential RNAs. The Plant Cell 6: 1441-1453
Soumounou Y and Laliberté JF (1994) Nucleic acid-binding properties of the P1 protein of turnip mosaic potyvirus produced in Escherichia coli. J Gen Virol 75: 2567-2573
Swaney S, Powers H, Goodwin J, Silva-Rosales L and Dougherty WG (1995) RNA-mediated resistance with non structural genes from the tobacco etch virus genome. Mol Plant Microbe Interact 8: 1004-1011
Vardi Y, Sela I, Edelbaum O, Livneh O, Kuznetsova L and Stram Y (1993) Plant transformed with a cistron of a potato virus Y protease (NIa) are resistant to virus infection. Proc Natl Acad Sci USA 90: 7513-7517
Verchot JM, Koonin EV and Carrington JC (1991) The 35-kDa protein from the N-terminus of the potyviral polyprotein functions as a third virus-encoded proteinase. Virology 185: 527-535
Verchot JM and Carrington JC (1995) Evidence that the potyvirus P1 proteinase functios intransas an accessory factor for genome amplification. J Virol 69: 3668-3674
Verwoerd TC, Deker BMM and Hoekema A (1989) A small scale procedure for the rapid isolation of plant RNAs. Nucl Acids Res 17: 2362
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Tavert-Roudet, G., Ravelonandro, M., Bachelier, JC. et al. Transgenic Nicotiana benthamiana plants containing the P1 gene of plum pox virus are resistant to virus challenge. European Journal of Plant Pathology 104, 103–107 (1998). https://doi.org/10.1023/A:1008671825361
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DOI: https://doi.org/10.1023/A:1008671825361