Abstract
Thirteen cows, which had been vaccinated as calves with strain 19, were revaccinated twice or three times as adults with 1×109 cfu of B. abortus strain RB51. Their serological responses following adult vaccination remained negative to conventional brucellosis surveillance tests. Vaccination with strain RB51 during the eighth month of pregnancy did not induce abortion, although strain RB51 was recovered from milk for up to 69 days after vaccination. In a parallel study, thirteen 8- to 10-month-old heifers were vaccinated as calves with 109 cfu of strain RB51. The heifers remained seronegative to conventional brucellosis surveillance tests but antibody responses to RB51 could be demonstrated using an indirect ELISA. This study showed that multiple vaccination with strain RB51 did not induce seroconversion to brucellosis surveillance tests. In addition, this study suggests that 109 cfu of strain RB51 is safe for use in pregnant cattle.
Similar content being viewed by others
REFERENCES
Alton, G.G., Jones, L.M., Angus, R.D. and Verger, J.M., 1988. Techniques for the Brucellosis Laboratory, (INRA, Paris)
Angus, R.D. and Barton, C.E., 1984. The production and evaluation of buffered plate antigen for use in a presumptive test for brucellosis. Development and Biological Standardization, 56, 349-356
Anonymous, 1998. Human exposure to Brucella abortus strain RB51. Kansas, 1997. Morbidity and MortalityWeekly Report of the Center for Disease Control, 47, 172-175
Buhrman, D.L., 1989. The behavior and effects of Brucella abortus rough strain RB51 in mice and cattle, (MS thesis, Virginia Polytechnic Institute and State University, Blacksburg)
Cheville, N.F., Stevens, M.G., Jensen, A.E., Tatum, F.M. and Halling, S.M., 1993. Immune responses and protection against infection and abortion in cattle experimentally vaccinated with mutant strains of Brucella abortus. American Journal of Veterinary Research, 54, 1591-1597
Cheville, N.F., Olsen, S.C., Jensen, A.E., Stevens, M.G., Palmer, M.V. and Florance, A.M., 1996. Effects of age at vaccination on efficacy of Brucella abortus strain RB51 to protect cattle against brucellosis. American Journal of Veterinary Research, 57, 1153-1156
Elzer, P.H., Enright, F.M., Colby, L., Hagius, S.D., Walker, J.V., Fatemi, M.B., Kopec, J.D., Beal, V. and Schurig, G.G., 1998. Oral vaccination of cattle with Brucella abortus strain RB51 protects against infection and abortion induced by virulent challenge. American Journal of Veterinary Research, 59, 1575-1578
Jensen, A.E., Ewalt, D.R., Cheville, N.F., Thoen, C.O. and Payeur, J.B., 1996. Determination of stability of B. abortus RB51 by use of genomic fingerprint, oxidative metabolism, and colonial morphology and differentiation of strain RB51 from Brucella abortus isolates from bison and elk. Journal of Clinical Microbiology, 34, 628-633
Jimenez de Bagues, M.P., Elzer, P.H., Jones, S.M., Blasco, J.M., Enright, F.M., Schurig, G.G. and Winter, A.J., 1994. Vaccination with Brucella abortus rough mutant RB51 protects BALB/C mice against virulent strains of Brucella abortus, Brucella melitensis and Brucella ovis. Infection and Immunity, 62, 4990-4996
Lord, V.R., Schurig, G.G., Cherwonogrodsky, J.W., Marcano, M.J. and Melendez, G.E., 1998. Field study of vaccination of cattle with Brucella abortus strains RB51 and 19 under high and low disease prevalence. American Journal of Veterinary Research, 59, 1016-1020
Nagy, L.K., Hignett, P.G. and Ironside, C.J.T., 1967. Bovine brucellosis: a study of an adult vaccinated infected herd. Veterinary Record, 81, 140-144
Nicoletti, P., 1981. Prevalence and persistence of Brucella abortus strain 19 infections and prevalence of other biotypes in vaccinated adult dairy cattle. Journal of the American Veterinary Medical Association, 178, 143-145
Nicoletti, P., 1990. Vaccination. In: K. Nielsen and J.R. Duncan (eds), Animal Brucellosis, (CRC Press, Boca Raton)
Nicoletti, P., 1991. Vaccination, bovine brucellosis. In: J.F. Frank (ed.), Networking in Brucellosis Research, (The United Nations University of Tokyo)
Palmer, M.V., Olsen, S.C. and Cheville, N.F., 1997. Safety and immunogenicity of Brucella abortus strain RB51 vaccine in pregnant cattle. American Journal of Veterinary Research, 58, 472-477
Schurig, G.G., Roop, R.M., Bagchi, T., Boyle, S., Buhrman, D. and Sriranganathan, N., 1991. Biological properties of RB51: a stable rough strain of Brucella abortus. Veterinary Microbiology, 28, 171-188
Schurig, G.G., Boyle, S. and Sriranganathan, N., 1995. Brucella abortus vaccine strain RB51: a brief review. Archivos de Medicina Veterinaria, 27, 19-22
Stevens, M.G. and Olsen, S.C., 1996. Antibody response to Brucella abortus 2308 in cattle vaccinated with Brucella abortus RB51. Infection and Immunity, 64, 1030-1034
Stevens, M.G., Hennager, D.C., Olsen, S.C. and Cheville, N.F., 1994. Serological response in diagnostic test for brucellosis in cattle vaccinated with Brucella abortus strain 19 or RB51. Journal of Clinical Microbiology, 32, 1065-1066
Stevens, M.G., Olsen, S.C., Palmer, M.V. and Pugh, G.W., 1996. Immune response and resistance to brucellosis in mice vaccinated orally with Brucella abortus RB51. Infection and Immunity, 64, 4534-4541
Uzal, F.A., Carrasco, E.A., Nielsen, K.H., Echaide, S. and Cabrera, R.F., 1996a. An indirect ELISA using a monoclonal anti IgG1 enzyme conjugate for the diagnosis of bovine brucellosis. Veterinary Microbiology, 52, 175-180
Uzal, F.A., Carrasco, E.A. and Nielsen, K.H., 1996b. Evaluation of a competitive ELISA for the diagnosis of bovine brucellosis. Veterinary Research Communications, 20, 421-426
Zambrano, A.J., Villava, F.M., Schurig, G.G. and Cherwonogrodzky, J.W., 1995. Resultados preliminares para la vacunacion de vacas preñadas con Brucella abortus cepa 19 o Brucella abortus cepa RB51. Archivos de Medicina Veterinaria, 27, 119-123
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Uzal, F., Samartino, L., Schurig, G. et al. Effect of Vaccination with Brucella abortus Strain RB51 on Heifers and Pregnant Cattle. Vet Res Commun 24, 143–151 (2000). https://doi.org/10.1023/A:1006468713614
Issue Date:
DOI: https://doi.org/10.1023/A:1006468713614