Abstract
Six-day-old alfalfa (Medicago sativa L.) seedlings were treated with auxin, abscisic acid (ABA), cytokinin and gibberellin to determine the effect of these plant hormones on induction of alcohol dehydrogenase (ADH). The ADH activity was increased at concentrations greater than 1 μM for auxin and ABA and 3 μM for cytokinin, respectively, and all increases were found within 6 h after treatments. However, ADH activity remained almost unchanged in the seedlings treated with gibberellin. At 100 μM doses, the activities in the seedlings were 4.0-, 3.6- and 2.1-fold greater than that of non-treated seedlings for auxin, ABA and cytokinin, respectively.
Similar content being viewed by others
References
Addicott FT and Van Steveninck RFM (1983) Summary: Significance of abscisic acid in the life of plants. In: Addicott FT (ed) Abscisic Acid. New York: Praeger Publishing, pp 581–586
Bruxelles de GL, Peacock WJ, Dennis ES and Dolferus R (1996) Abscisic acid induces the alcohol dehydrogenase gene in Arabidopsis. Plant Physiol 111: 381–391
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 7: 248–254
Davies DD (1980) Anaerobic metabolism and the production of organic acids. In: Davies DD (ed) The Biochemistry of Plants Vol. 2. New York: Academic Press, pp 581–611
Dolferus R, Ellis M, Bruxelles de G, Trevaskis B, Hoeren F, Dennis ES and Peacock WJ (1997) Strategies of gene action in Arabidopsis during hypoxia. Annal Bot 79: 21–31
Good AG and Muench DG (1993) Long-term anaerobic metabolism in root tissue: Metabolic products of pyruvate metabolism. Plant Physiol 101: 1163–1168
Guy CL (1990) Cold acclimation and freezing stress tolerance: Role of protein metabolism. Annu Rev Plant Physiol Plant Mol Biol 41: 187–223
Hanson AD, Jacobsen JV and Zwar JA (1984) Regulated expression of three alcohol dehydrogenase genes in barely aleurone layers. Plant Physiol 75: 573–581
Hwang S-Y and Van Toai TT (1991) Abscisic acid induces anaerobiosis tolerance in corn. Plant Physiol 97: 593–597
Jacobsen JV (1983) Regulation of protein synthesis in aleurone cells by gibberellin and abscisic acid. In: Crozier A (ed) The Biochemistry and Physiology of Gibberellins. New York: Praeger Publishing, pp 159–187
Jarillo JA, Leyva A, Salinas J, and Marínez-Zapater JM (1993) Low temperature induces the accumulation of alcohol dehydrogenase mRNA in Arabidopsis thaliana, a chillingtolerant plant. Plant Physiol 101: 833–837
Kato-Noguchi H and Watada AE (1997) Effects of low-oxygen atmosphere on ethanolic fermentation in fresh-cut carrots. J Amer Soc Hort Sci 122: 107–111
Ke D, Yahia E, Mateos M and Kader AA (1994) Ethanolic fermentation of 'Bartlett' pears as influenced by ripening stage and atmospheric composition. J Amer Soc Hort Sci 119: 976–982
Kennedy RA, Rumpho ME and Fox TC (1992) Anaerobic metabolism in plants. Plant Physiol 100: 1–6
Lange OL, Nobel PS, Osmond CB and Ziegler H (1981) Introduction: Perspectives in ecological plant physiology. In: Lange OL, Nobel PS, Osmomd CB and Ziegler H (eds) Physiological Plant Ecology I. New York: Springer-Verlag, pp 1–9
Perata P and Alpi A (1991) Ethanol-induced injuries to carrot cells: The role of acetaldehyde. Plant Physiol 95: 748–752
Ricard B, Couée I, Raymond P, Saglio PH, Saint-Ges V and Pradet A (1994) Plant metabolism under hypoxia and anoxia. Plant Physiol & Biochem 32: 1–10
Roberts JKM, Callis J, Jardetzky O, Walbot V and Freeling M (1984) Cytoplasmic acidosis as a determinant of flooding intolerance in plants. Proc Natl Acad Sci USA 81: 6029–6033
Sachs MM and Ho T-HD (1986) Alteration of gene expression during environmental stress in plants. Annu Rev Plant Physiol 37: 363–376
Sánchez-Serrano JJ, Amati S, Ebneth M, Hildmann T, Mertens R, Pena-Cortés H, Prat S and Willmitzer L (1991) The involvement of ABA in wound responses of plants. In: Davies WJ and Jones HG (eds) Abscisic Acid, Physiology and Biochemistry. Oxford, UK: Bios Scientific Publishers, pp 210–216
Xie Y and Wu R (1989) Rice alcohol dehydrogenase genes: Anaerobic induction, organ specific expression and characterization of cDNA clones. Plant Mol Biol 13: 53–68
Zeevaart JAD and Creelman RA (1988) Metabolism and physiology of abscisic acid. Annu Rev Plant Physiol Plant Mol Biol 39: 439–473
Rights and permissions
About this article
Cite this article
Kato-Noguchi, H. Induction of alcohol dehydrogenase by plant hormones in alfalfa seedlings. Plant Growth Regulation 30, 1–3 (2000). https://doi.org/10.1023/A:1006253615894
Issue Date:
DOI: https://doi.org/10.1023/A:1006253615894