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Picosecond laser-fluorometer with simultaneous time and wavelength resolution for monitoring decay spectra of photoinhibited Photosystem II particles at 277 K and 10 K

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Abstract

The present study describes a novel fluorometer system which permits the simultaneous monitoring of the time and wavelength dependence of chlorophyll fluorescence in the picosecond time domain. The key element of this equipment is a microchannel-plate photomultiplier with delay-line anode. The comparatively short acquisition times in combination with full spectral and temporal resolution of this device are of high advantage, especially for measurements on photosynthetic samples at cryogenic temperatures. For a convenient numerical data evaluation of complex overlapping spectra a decay-associated gaussian deconvolution technique was installed by developing a fitting program with graphical user-interface. In order to illustrate the potential of the new set-up, surface plots and decay spectra gathered from measurements with anaerobically photoinhibited Photosystem II particles at 277 K and 10 K are presented.

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Authors and Affiliations

  1. Optisches Institut, Technische Universität Berlin, Strasse des 17. Juni 135, 10623, Berlin, Germany

    Axel Bergmann & Hans-Joachim Eichler

  2. Max-Volmer Institut für Biophysikalische Chemie und Biochemie, Technische Universität Berlin, Straße des 17. Juni 135, 10623, Berlin, Germany

    Hann-Jörg Eckert & Gernot Renger

Authors
  1. Axel Bergmann
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  2. Hans-Joachim Eichler
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  3. Hann-Jörg Eckert
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  4. Gernot Renger
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Correspondence to Hann-Jörg Eckert.

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Bergmann, A., Eichler, HJ., Eckert, HJ. et al. Picosecond laser-fluorometer with simultaneous time and wavelength resolution for monitoring decay spectra of photoinhibited Photosystem II particles at 277 K and 10 K. Photosynthesis Research 58, 303–310 (1998). https://doi.org/10.1023/A:1006181908149

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  • DOI: https://doi.org/10.1023/A:1006181908149

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