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Plant regeneration from cocoyam callus derived from shoot tips and petioles

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Abstract

The effects of various growth regulators on morphogenesis from cocoyam tissues (Xanthosoma sagittifolium) were investigated. Calluses were initiated from shoot tip and petiole explants and proliferated on medium containing 1.36 μM dicamba. Callus production was significantly greater from petioles than from shoot tips. Thidiazuron (0.045 μM) enhanced callus production when dicamba (13.5 μM) was used, and was more favorable to petioles than shoot tips. Friable shoot tip callus was subcultured into liquid media containing either 1.36 μM dicamba alone, 1.35 μM 2,4-D + 0.46 μM kinetin or 1.36 μM dicamba + 0.46 μM kinetin to induce adventive regeneration. Tissues producing single or aggregated shoot buds were subcultured into media containing 0, 0.049 and 0.49 μM 2-isopentenyladenine where bud multiplication and shoot regeneration were observed. Bud aggregates were formed from callus in liquid cultures containing 1.36 μM dicamba, 1.36 μM dicamba + 0.46 μM kinetin or 1.35 μM 2,4-D + 0.46 μM kinetin. Shoot bud clumps which remained green produced shoots, daughter buds, and plantlets in stationary and agitated liquid media containing 0, 0.049 and 0.49 μM 2iP.

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Authors and Affiliations

  1. Department of Plant and Soil Science, Alabama A & M University, P.O. Box 1208, Normal, AL, 35762, USA

    Leopold M. Nyochembeng & Stephen Garton

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  1. Leopold M. Nyochembeng
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  2. Stephen Garton
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Nyochembeng, L.M., Garton, S. Plant regeneration from cocoyam callus derived from shoot tips and petioles. Plant Cell, Tissue and Organ Culture 53, 127–134 (1998). https://doi.org/10.1023/A:1006084207636

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