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Molecular cloning in Arabidopsis thaliana of a new protein phosphatase 2C (PP2C) with homology to ABI1 and ABI2

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Abstract

We report the cloning of both the cDNA and the corresponding genomic sequence of a new PP2C from Arabidopsis thaliana, named AtP2C-HA (for homology to ABI1/ABI2). The AtP2C-HA cDNA contains an open reading frame of 1536 bp and encodes a putative protein of 511 amino acids with a predicted molecular mass of 55.7 kDa. The AtP2C-HA protein is composed of two domains, a C-terminal PP2C catalytic domain and a N-terminal extension of ca. 180 amino acid residues. The deduced amino acid sequence is 55% and 54% identical to ABI1 and ABI2, respectively. Comparison of the genomic structure of the ABI1, ABI2 and AtP2C-HA genes suggests that they belong to a multigene family. The expression of the AtP2C-HA gene is up-regulated by abscisic acid (ABA) treatment.

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Authors and Affiliations

  1. Instituto de Biologia Molecular y Celular de Plantas, Universidad Politécnica - C.S.I.C., Camino de Vera, 46022, Valencia, Spain

    Pedro L. Rodriguez & Martin P. Leube

  2. TU-München, Lehrstuhl für Botanik, Arcisstrasse 16, 80333, München, Germany

    Erwin Grill

Authors
  1. Pedro L. Rodriguez
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  2. Martin P. Leube
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  3. Erwin Grill
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Rodriguez, P.L., Leube, M.P. & Grill, E. Molecular cloning in Arabidopsis thaliana of a new protein phosphatase 2C (PP2C) with homology to ABI1 and ABI2. Plant Mol Biol 38, 879–883 (1998). https://doi.org/10.1023/A:1006012218704

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  • DOI: https://doi.org/10.1023/A:1006012218704

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