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Digitization of DGGE (denaturing gradient gel electrophoresis) profile and cluster analysis of microbial communities

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Abstract

Denaturing gradient gel electrophoresis (DGGE) of 16S rDNA profiles were objectively digitized using an image analyzer; the individual microbial species in a community can thus be precisely quantified. The similarity between various microbial communities was compared to the digitized DGGE profiles using the cluster analyses technique. The microbial community in a biofilm was considerably different from that in suspended sludge obtained from the same system.

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Zhang, T., Fang, H.H. Digitization of DGGE (denaturing gradient gel electrophoresis) profile and cluster analysis of microbial communities. Biotechnology Letters 22, 399–405 (2000). https://doi.org/10.1023/A:1005680803442

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  • DOI: https://doi.org/10.1023/A:1005680803442

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