Skip to main content
SpringerLink
Log in

Photoproduction of indole 3-acetic acid by Rhodobacter sphaeroides from indole and glycine

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Rhodobacter sphaeroides photoproduced indole 3-acetic acid (IAA) when the precursor L-tryptophan was added to the basal medium. Of the other organic carbon sources that influenced IAA formation from L-tryptophan, α-ketoglutaric acid gave the maximum formation of 530 mg 1−1 in less than 24 h of illuminated anaerobic incubation. IAA was also produced from indole, glycine and glucose together by Rb. sphaeroides under photoanaerobic conditions yielding 61 mg l−1 within 30 fh.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anderson GM (1975) Quantification of tryptophan in rat faeces by thin layer chromatography. J. Chromatogr. 105: 323–328.

    Google Scholar 

  • Biebl H, Pfennig N (1981) Isolation of members of family Rhodospirillaceae. In: Starr MP, Stolp H, Truper HG, Balows A, Schlegel HG, eds. The Prokaryotes, Vol. 1. New York: Springer Verlag, pp. 267–273.

    Google Scholar 

  • Forni C, Riou J, Grilli CM, Tel-Or E (1992) Indole-3-acetic acid (IAA) production by Arthrobacter species isolated from Azolla. J. Gen. Microbiol. 138: 377–381.

    Google Scholar 

  • Koga J, Syono K, Ichikawa T, Adachi T (1994) Involvement of L-tryptophan amino transferase in indole-3-acetic acid biosynthesis in Enterobacter cloacae. Biochim. Biophys. Acta 1209: 241–247.

    Google Scholar 

  • Kupfer D, Atkinson DE (1964) Quantitative method for determination of indole, tryptophan and anthranilic acid in the same aliquot. Anal. Biochem. 8: 82–94.

    Google Scholar 

  • Manulis S, Shafrir H, Epstein E, Lichter A, Barach I (1994) Biosynthesis of indole-3-acetic acid via indole-3-acetamide pathway in Streptomyces spp. Microbiology 140: 1045–1050.

    Google Scholar 

  • Oberhansli T, Defago G, Haas D (1991) Indole-3-acetic acid synthesis in the biocontrol strain CHAO of Pseudomonas fluorescens; role of tryptophan side chain oxidase. J. Gen. Microbiol. 137: 2273–2279.

    Google Scholar 

  • Rajasekhar N, Sasikala Ch, Ramana ChV (1998) Photobiotransformation of indole to its value-added derivatives by Rhodobacter sphaeroides OU5. J. Ind. Microbiol. Biotech. 20: 177–179.

    Google Scholar 

  • Sasikala Ch, Ramana ChV (1995a) Biotechnological potentials of anoxygenic phototrophic bacteria. 1. Production of single cell protein, vitamins, ubiquinones, hormones and enzymes and use in waste treatment. Adv. Appl. Microbiol. 41: 173–226.

    Google Scholar 

  • Sasikala Ch, Ramana ChV (1995b) Biotechnological potentials of anoxygenic phototrophic bacteria. 2. Biopolyesters, biopesticide, biofuel and biofertilizer. Adv. Appl. Microbiol. 41: 227–278.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Environmental Microbial Biotechnology Laboratory, Centre for Environment, IPGS & R, J.N.T. University, Mahaveer Marg, Hyderabad, 500 028, India

    N. Rajasekhar, Ch. Sasikala & Ch.V. Ramana

Authors
  1. N. Rajasekhar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ch. Sasikala
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ch.V. Ramana
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rajasekhar, N., Sasikala, C. & Ramana, C. Photoproduction of indole 3-acetic acid by Rhodobacter sphaeroides from indole and glycine. Biotechnology Letters 21, 543–545 (1999). https://doi.org/10.1023/A:1005515906321

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005515906321

Search

Navigation