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A thermostable glucose isomerase having a relatively low optimum pH: study of activity and molecular cloning of the corresponding gene

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Abstract

A thermostable glucose isomerase from a newly isolated thermophilic Streptomyces sp. SK strain, had a wide pH range with an optimum of 6 at 60°C and 6.4 at 90°C. It was optimally active at 95°C and completely stable at 80°C for at least 5.5 h with a half-life of 5 h at 90°C. Using E.coli as a host strain and an internal fragment of xylA of S. olivochromogenes as a probe, a 6.5 kb DNA fragment from Streptomyces sp. SK was cloned. This fragment carries the entire xylA gene since the recombinant plasmid complements the E.coli xyl-5 mutant strain HB101. © Rapid Science Ltd. 1998

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Authors and Affiliations

  1. Centre de Biotechnologie de Sfax, Sfax, Tunisia

    Karima Srih-Belghith & Samir Bejar

Authors
  1. Karima Srih-Belghith
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  2. Samir Bejar
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Srih-Belghith, K., Bejar, S. A thermostable glucose isomerase having a relatively low optimum pH: study of activity and molecular cloning of the corresponding gene. Biotechnology Letters 20, 553–556 (1998). https://doi.org/10.1023/A:1005393510435

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  • DOI: https://doi.org/10.1023/A:1005393510435

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