Skip to main content
SpringerLink
Log in

Halonatronum saccharophilum gen. nov. sp. nov.: A New Haloalkaliphilic Bacterium of the Order Haloanaerobiales from Lake Magadi

  • Published:
Microbiology Aims and scope Submit manuscript

Abstract

A new alkaliphilic and moderately halophilic chemoorganotrophic anaerobic bacterium (strain Z-7986), which is spore-forming, rod-shaped, and has a gram-negative cell wall pattern, was isolated from the coastal lagoon mud of the highly mineralized Lake Magadi (Kenya). The organism is an obligatorily carbonate- and sodium chloride-dependent motile peritrichously flagellated rod that grows within a 3–17% NaCl concentration range (with an optimum at 7–12% NaCl) and within a pH range of 7.7–10.3 (with an optimum at pH values of 8–8.5). It is a moderate thermophile with a broad temperature optimum at 36–55°C; maximum growth temperature is 60°C. The bacterium catabolizes glucose, fructose, sucrose, maltose, starch, glycogen, N-acetyl-D-glucosamine, and, to a slight degree, peptone and yeast extract. Its anabolism requires yeast extract or casamino acids. Glucose fermentation yields formate, acetate, ethanol, H2, and CO2. The bacterium is sulfide-tolerant and capable of the nonspecific reduction of S0 to H2S. The G+C content of the DNA is 34.4 mol %. The analysis of the 16S rRNA sequence revealed that strain Z-7986 belongs to the order Haloanaerobiales and represents a new genus in the family Halobacteroidaceae. We suggest the name Halonatronum saccharophilum gen. nov. sp. nov. The type strain of this species is Z-7986T (= DSM13868, = Uniqem*211).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Rainey, F.A., Zhilina, T.N., Boulygina, E.S., Stackebrandt, E., Tourova, T.P., and Zavarzin, G.A., The Taxonomic Status of the Fermentative Halophilic Anaerobic Bacteria: Description of Haloanaerobiales ord. nov., Halobacteroidaceae fam. nov., Orenia gen. nov., and Further Taxonomic Rearrangements at the Genus and Species Level, Anaerobe, 1995, vol. 1, pp. 185–199.

    Google Scholar 

  2. Zhilina, T.N. and Zavarzin, G.A., Anaerobic Degradative Bacteria in the Halophilic Cyanobacterial Community, Zh. Obshch. Biol., 1991, vol. 52, pp. 302–318.

    Google Scholar 

  3. Zhilina, T.N., Zavarzin, G.A., Detkova, E.N., and Rainey, F.A., Natroniella acetigena gen. nov., sp. nov., an Extremely Haloalkaliphilic, Homoacetic Bacterium: A New Member of Haloanaerobiales, Curr. Microbiol., 1996, vol. 32, pp. 320–326.

    Google Scholar 

  4. Zhilina, T.N. and Zavarzin, G.A., A New Extremely Halophilic Homoacetic Bacterium Acetohalobium arabaticum gen. nov., sp. nov., Dokl. Akad. Nauk SSSR, 1990, vol. 311, pp. 745–747.

    Google Scholar 

  5. Zhilina, T.N. and Zavarzin, G.A., Alkaliphilic Anaerobic Community at pH 10, Curr. Microbiol., 1994, vol. 29, pp. 109–112.

    Google Scholar 

  6. Jones, B.E., Grant, W.D., Duckworth, A.W., and Owenson, G.G., Microbial Diversity of Soda Lake, Extremophiles, 1998, vol. 2, pp. 191–200.

    Google Scholar 

  7. Tourova, T.P., Garnova, E.S., and Zhilina, T.N., Phylogenetic Diversity of Alkaliphilic Anaerobic Saccharolytic Bacteria Isolated from Soda Lakes, Mikrobiologiya, 1999, vol. 68, pp. 701–709.

    Google Scholar 

  8. Zhilina, T.N., Zavarzin, G.A., Rainey, F.A., Kevbrin, V.V., Kostrikina, N.A., and Lysenko, A.M., Spirochaeta alkalica sp. nov., Spirochaeta africana sp. nov., and Spirochaeta asiatica sp. nov., Alkaliphilic Anaerobes from the Continental Soda Lakes in Central Asia and East African Rift, Int. Syst. Bacteriol, 1996, vol. 46, pp. 305–312.

    Google Scholar 

  9. Zavarzin, G.A., Zhilina, T.N., and Kevbrin, V.V., The Alkaliphilic Microbial Community and Its Functional Diversity, Mikrobiologiya, 1999, vol. 68, pp. 579–599.

    Google Scholar 

  10. Kevbrin, V.V. and Zavarzin, G.A., The Effect of Sulfur Compounds on the Growth of the Halophilic Homoacetic Bacterium Acetohalobium arabaticum, Mikrobiologiya, 1992, vol. 61, pp. 812–817.

    Google Scholar 

  11. Wolin, E.A., Wolin, M.J., and Wolfe, R.S., Formation of Methane by Bacterial Extracts, J. Biol. Chem., 1963, vol. 238, pp. 2882–2886.

    Google Scholar 

  12. Hanson, R. and Phillips, J., Chemical Composition of the Bacterial Cell, in Manual of Methods for General Bacteriology, Gerhardt, P. et al., Eds., Washington: Am. Soc. Microbiol., 1981.

    Google Scholar 

  13. Lang, E. und Lang, N., Spezifische Farbreaktion zum direkten Nachweis der Ameisensäure, Fres. Z. Anal. Chem., 1972, vol. 260, no. 1, pp. 8–10.

    Google Scholar 

  14. Trüper, H.G. and Schlegel, H.G., Sulfur Metabolism in Thiorhodaceae: Quantitative Measurements on Growing Cells of Chromatium okenii, Antonie van Leeuwenhoek, 1964, vol. 30, pp. 225–238.

    Google Scholar 

  15. Pfennig, N. and Wagener, S., An Improved Method of Preparing Wet Mounts for Photomicrographs of Microorganisms, J. Microbiol. Methods, 1986, vol. 4, pp. 303–306.

    Google Scholar 

  16. Van de Peer, Y. and De Wachter, R., TREECON for Windows: A Software Package for the Construction and Drawing of Evolutionary Trees for the Microsoft Windows Environment, Comput. Appl. Biosci., 1994, vol. 10, pp. 569–570.

    Google Scholar 

  17. Felsenstein, J., PHYLIP Manual Version 3.3., University Herbarium, Univ. of California, Berkeley, 1990.

    Google Scholar 

  18. Zhilina, T.N., Tourova, T.P., Kuznetsov, B.B., Kostrikina, N.A., and Lysenko, A.M., Orenia sivashensis sp. nov., a New Moderately Halophilic Anaerobic Bacterium from Lake Sivash Lagoons, Mikrobiologiya, 1999, vol. 68, pp. 517–527.

    Google Scholar 

  19. Ravot, G., Magot, M., Olliver, B., Patel, B.K.C., Ageron, E., Grimont, P.A.D., Thomas, P., and Garcia, J.L., Haloanaerobium congolense sp. nov., an Anaerobic, Thiosulfate and Sulfur-reducing Bacterium from an African Oil Field, FEMS Microbiol. Lett., 1997, vol. 147, pp. 81–88.

    Google Scholar 

  20. Moune, S., Eatock, C., Matheron, R., Willison, J.C., Hirschler, A., Herbert, R., and Caumette, P., Orenia salinaria sp. nov., a Fermentative Bacterium Isolated from Anaerobic Sediments of Mediterranean Salterns, Int. J. Syst. Evol. Microbiol., 2000, vol. 50, pp. 721–729.

    Google Scholar 

  21. Oren, A., Pohla, H., and Stackebrandt, E., Transfer of Clostridium lortetii to a New Genus, Sporohalobacter gen. nov. as Sporohalobacter lortetii comb. nov. and Description of Sporohalobacter marismortui sp. nov., Syst. Appl. Microbiol., 1987, vol. 9, pp. 239–246.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117811, Russia

    T. N. Zhilina, E. S. Garnova, T. P. Tourova, N. A. Kostrikina & G. A. Zavarzin

Authors
  1. T. N. Zhilina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. S. Garnova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. P. Tourova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. A. Kostrikina
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. A. Zavarzin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhilina, T.N., Garnova, E.S., Tourova, T.P. et al. Halonatronum saccharophilum gen. nov. sp. nov.: A New Haloalkaliphilic Bacterium of the Order Haloanaerobiales from Lake Magadi. Microbiology 70, 64–72 (2001). https://doi.org/10.1023/A:1004896905579

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1004896905579

Search

Navigation