Abstract
The oxidative consumption of methane (CH4) generally proceeds with a significant isotope fractionation, and isotopic variations in CH4 observed in sulfate-containing anaerobic sediments have often been interpreted as an indicator of anaerobic methane oxidation at the expense of sulfate. However, we found variations in δ13C value of CH4 depending on sulfate availability in tropical swamp sediments, in which no anaerobic CH4 oxidation was detected. In one sediment, the range of δ13C variation due to sulfate was as large as 20‰. The variations in δ13C of decomposed organic matter and CO2 failed to explain the variation in CH4 δ13C. We postulate a syntrophic linkage between sulfate-reducing and methanogenic bacteria via acetate as a mechanism of the observed δ'13C variation.
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References
Alperin, M. J., N. E. Blair, D. B. Albert, T. M. Hoehler & C. S. Martens, 1992. Factors that control the stable carbon isotopic composition of methane produced in an anoxic marine sediment. Global biogeochem. Cycles 6: 271–291.
Anderson, B. L., 1996. Modeling isotopic fractionation in systems with multiple sources and sinks with application to atmospheric CH4.Global biogeochem. Cycles 10: 191–196.
Barker, J. F. & P. Fritz, 1981. Carbon isotope fractionation during microbial methane oxidation. Nature 293: 289–291.
Coleman, D. D., J. B. Risatti & M. Schoell, 1981. Fractionation of carbon and hydrogen isotopes by methane-oxidizing bacteria. Geochim. cosmochim. Acta 45: 1033–1037.
Hansen, T. A., 1993. Carbon metabolism of sulfate-reducing bacteria. In Odom, J. M. & R. Singleton Jr. (ed.), The Sulfate-Reducing Bacteria: Contemporary Perspectives. Springer-Verlag, New York: 21–40.
Kalbasi, M. & M. A. & Tabatabai, 1985. Simultaneous determination of nitrate, chloride, sulfate, and phosphate in plant materials by ion chromatography. Commun. Soil Sci. Plant Anal. 16: 787–800.
King, G. M., 1992. Ecological aspects of methane oxidation, a key determinant of global methane dynamics. Adv.Microb. Ecol. 12: 431–468.
Miyajima, T., E. Wada, Y. T. Hanba & P. Vijarnsorn, 1997. Anaerobic mineralization of indigenous organic matters and methanogenesis in tropical wetland soils. Geochim. cosmochim. Acta 61: 3739–3751.
Mook W. G., J. C. Bommerson & W. H. Staverman, 1974.Carbon isotope fractionation between dissolved bicarbonate and gaseous carbon dioxide. Earth. Planet. Sci. Lett. 22: 169–176.
Oremland, R. S., 1988. Biogeochemistry of methanogenic bacteria. In A. J. B. Zehnder, (ed.), Biology of Anaerobic Microorganisms. Wiley-Liss, New York: 641–705.
Oremland, R. S. & D. G. Capone, 1988. Use of 'specific’ inhibitors in biogeochemistry and microbial ecology.Adv. Microb. Ecol. 10: 285–383.
Ricci M. P, D. A. Merritt, K. H. Freeman & J. M. Hayes, 1994. Acquisition and processing of data for isotope-ratio-monitoring mass spectrometry. Org. Geochem. 21: 561–571.
Stevens, C. M., 1993.Isotope abundances in the atmosphere and sources. In: Khalil, M. A. K. (ed.), Atmospheric Methane: Sources, Sinks, and Role in Global Change. Springer-Verlag, Berlin: 62–88.
Sugimoto, A. & E. Wada, 1993. Carbon isotope composition of bacterial methane in a soil incubation experiment: Contribution of acetate and CO2/H2. Geochim. cosmochim. Acta 57: 4015–4027.
Sugimoto, A., Xu Hong & E. Wada, 1991. Rapid and simple measurement of carbon isotope ratio of bubble methane using GC/C/IRMS. Mass Spectrosc.39: 261–266.
Vijarnsorn, P., 1992. Problems related to coastal swamp land development in southern Thailand. In Kyuma, K., P. Vijarnsorn & A. Zakaria, (eds.), Coastal Lowland Ecosystems in Southern Thailand and Malaysia. Kyoto University, Kyoto: 3–16.
Whiticar, M. J., 1993. Stable isotopes and global budgets. In Khalil, M. A. K. (ed.), Atmospheric Methane: Sources, Sinks, and Role in Global Change. Springer-Verlag, Berlin, Germany: 138–167.
Whiticar, M. J., E. Faber, and M. Schoell, 1986. Biogenic methane formation in marine and freshwater environments: CO2 reduction vs. acetate fermentation–Isotope evidence. Geochim. cosmochim. Acta 50: 693–709.
Widdel, F. 1988. Microbiology and ecology of sulfate-and sulfurreducing bacteria. In Zehnder, A. J. B. (ed.), Biology of Anaerobic Microorganisms. Wiley-Liss, New York: 469–585.
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Miyajima, T., Wada, E. Sulfate-induced isotopic variation in biogenic methane from a tropical swamp without anaerobic methane oxidation. Hydrobiologia 382, 113–118 (1998). https://doi.org/10.1023/A:1003409812416
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DOI: https://doi.org/10.1023/A:1003409812416