Abstract
Type VI collagen was revealed by high-resolution immunocytochemistry in renal glomeruli from short- and long-term streptozotocin-injected hyperglycaemic rats and from their age-matched normoglycaemic controls. The labellings obtained over the glomerular basement membrane and the mesangial matrix were assessed by quantitative evaluations. The labellings over the glomerular basement membrane were low and sparse in the young normoglycaemic animals but became consistent and increased in intensity with age and in both the short- and long-term diabetic animals. For the mesangial matrix, this was labelled more systematically, and its intensity increased with age and in the short-term hyperglycaemic animals. For the long-term hyperglycaemic animals, the intensities of labelling resembled those of their age-matched controls. These results indicate that type VI collagen appears to be a minor constituent of the extracellular matrix of the rat glomeruli, rather concentrated in the mesangial area in the young control animal. Concomitant with the general modifications of the extracellular matrix occurring with age and diabetes, this component increases, but apparently not with the length of the hyperglycaemic state. © Chapman & Hall
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bendayan, M. (1985) Alteration in the distribution of type IV collagen in glomerular basal laminae in diabetic rats as revealed by immunocytochemistry and morphometrical approach. Diabetologia 28, 373–8.
Bendayan, M. (1995) Colloidal gold post-embedding immunocytochemistry. Prog. Histochem. Cytochem. 29, 1–163.
Bolton, W.K., Benton, F.R., Maclay, J.G. & Sturgill, B.C. (1976) Spontaneous glomerular sclerosis in aging Sprague-Dawley rats. Am. J. Pathol. 85, 277–302.
Chung, E., Rhodes, R.K. & Miller, E. J. (1976) Isolation of three collagenous components of probable basement membrane origin from several tissues. Biochem. Biophys. Res. Commun. 71, 1167–74.
Couchman, J.R., Beavan, L.A. & Mccarthy, K.J. (1994) Glomerular matrix: synthesis, turnover and role in mesangial expansion. Kidney Int. 45, 328–35.
Couser, W.G. & Stilmant, M.M. (1975) Mesangial lesions and focal glomerular sclerosis in the aging rat. Lab. Invest. 33, 491–501.
Darmady, E.M., Offer, J. & Woodhouse, M.A. (1973) The parameters of the ageing kidney. J. Pathol. 109, 195–207.
Desjardins, M. & Bendayan, M. (1990) Ultrastructural distribution of glomerular basement membrane components in experimental diabetes. Diabetes Res. 14, 65–73.
Fukui, M., Nakamura, T., Ebihara, I., Shirato, I., Tomino, Y. & Koide, H. (1992) ECM gene expression and its modulation by insulin in diabetic rats. Diabetes 41, 1520–7.
Hessle, H. & Engvall, E. (1984) Type VI collagen. Studies on its localization, structure and biosynthetic form with monoclonal antibodies. J. Biol. Chem. 259, 3955–61.
Hirose, K., Osterby, R., Nozawa, M. & Gundersen, H.J. (1982) Development of glomerular lesions in experimental long-term diabetes in the rat. Kidney Int. 21, 889–95.
Inoue, S. & Bendayan, M. (1995) High-resolution ultrastructural study of the rat glomerular basement membrane in long-term experimental diabetes. Ultrastruct. Pathol. 19, 175–85.
Jander, R., Rauterberg, J., Voss, B. & Von Bassewitz, D.B. (1981) A cysteine-rich collagenous protein from bovine placenta. Isolation of its constituent polypeptide chains and some properties of the non-denaturated protein. Eur. J. Biochem. 114, 17–25.
Karkavelas, G., Kefalides, N.A., Amenta, P.S. & Matinez-Hernandez, A. (1988) Comparative ultrastructural localization of collagen type III, IV, VI and laminin in rat uterus and kidney. J. Ultrastruct. Mol. Struct. Res. 100, 137–55.
Karttunen, T., Risteli, J., Autio-Harmainen, H. & Risteli, L. (1986) Effect of age and diabetes on type IV collagen and laminin in human kidney cortex. Kidney Int. 30, 586–91.
Kashtan, C.E. & Kim, Y. (1992) Distribution of the á1 and α2 chains of collagen IV and collagen V and VI in Alport syndrome. Kidney Int. 42, 115–26.
Keene, D.R., Engvall, E. & Glanvil, R.W. (1988) Ultrastuctural of type VI collagen in human skin and cartilage suggests an anchoring function for this FIlamentous network. J. Cell Biol. 107, 1995–2006.
Leehey, D. J., Song, R.H., Alavi, N. & Singh, A.K. (1995) Decreased degradative enzymes in mesangial cells cultured in high glucose media. Diabetes 44, 929–35.
Mclennan, S.V., Fisher, E. J., Yue, D.K. & Turtle, J.R. (1994) High glucose concentration causes a decrease in mesangium degradation. A factor in pathogenesis of diabetic nephropathy. Diabetes 43, 1041–5.
Magro, G., Grasso, S., Colombatti, A. & Lopes, M. (1996) Immunohistochemical distribution of type VI collagen in developing human kidney. Histochem. J. 28, 385–90.
Mohan, P.S., Carter, W.G. & Spiro, R.G. (1990) Occurrence of type VI collagen in extracellular matrix of renal glomeruli and its increase in diabetes. Diabetes 39, 31–7.
Nerlich, A. & Schleicher, E. (1991) Immunohistochemical localization of extracellular matrix components in human diabetic glomerular lesions. Am. J. Pathol. 139, 889–99.
Nerlich, A.G., Schleicher, E.D., Wiest, I., Specks, V. & Timpl, R. (1994) Immunohistochemical localization of collagen VI in diabetic glomeruli. Kidney Int. 45, 1648–56.
Peleg, I., Greenfeld, Z., Cooperman, H. & Shoshan, S. (1993) Type I and type III collagen mRNA levels in kidney regions of old and young rats. Matrix 13, 281–7.
Prockop, D. J. & Kivirikko, K.I. (1995) Collagens: molecular biology, diseases, and potentials for therapy. Annu. Rev. Biochem. 64, 403–34.
Pugliese, G., Pricci, F., Pugliese, F., Mene, P., Lenti, L., Andreani, D., Galli, G., Casini, A., Bianchi, S., Rotella, C.M. & Di Mario, U. (1994) Mechanisms of glucose-enhanced extracellular matrix accumulation in rat glomerular mesangial cells. Diabetes 43, 478–90.
Quaglino, D., Fornieri, C., Nanney, L.B. & Davidson, J.M. (1993) Extracellular matrix modiFIcation in rat tissues of different ages. Matrix 13, 481–90.
Reckelhoff, J.F., Tygart, V.L., Mitias M.M. & Walcott, J.L. (1993) STZ-induced diabetes results in decreased activity of glomerular cathepsin and metalloprotease in rats. Diabetes 42, 1425–32.
Reiser, K.M., Hennessy, S.M. & Last, J.A. (1987) Analysis of age-associated changes in collagen crosslinking in the skin and lung in monkeys and rats. Biochem. Biophys. Acta 926, 339–48.
Rupprecht, H.D., SchÖcklmann, H.O. & Sterzel, R.B. (1996) Cell-matrix interactions in the glomerular mesangium. Kidney Int. 49, 1575–82.
Schaefer, L., Schaefer, R.M., Ling, H., Teschner, M. & Heidland, A. (1994) Renal proteinases and kidney hypertrophy in experimental diabetes. Diabetologia 37, 567–571.
Schleicher, E., Kolm, V., Ceol, M. & Nerlich, A. (1996) Structural and functional changes in diabetic glomerulopathy. Kidney Blood Press. Res. 19, 305–15.
von der Mark, H., Aumailley, M., Wick, G., Fleischmajer, R. & Timpl, R. (1984) Immunochemistry, genuine size and tissue localization of collagen VI. Eur. J. Biochem. 142, 493–502.
Yamauchi, M., Woodley, D.T. & Mechanic, G.L. (1988) Aging and crosslinking of skin collagen. Biochem. Biophys. Res. Commun. 152, 898–903.
Zhu, D., Kim, Y., Steffes, M.W., Groppoli, T. J., Butkowski, R. J. & Mauer, S.M. (1994) Application of electron microscopic immunocytochemistry to human kidney: distribution of type IV and type VI collagen in normal human kidney. J. Histochem. Cytochem. 42, 577–84.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Regoli, M., Magro, G., Grasso, S. et al. Type VI collagen in glomeruli of short- and long-term experimental diabetic rats. Histochem J 30, 13–20 (1998). https://doi.org/10.1023/A:1003258311438
Issue Date:
DOI: https://doi.org/10.1023/A:1003258311438