biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 43:205-209, 2000 | DOI: 10.1023/A:1002791824350

Variability of Storage Proteins and Esterase Isozymes in Vicia Sativa Subspecies

A.S. Haider1, A.R. El-Shanshoury1
1 Department of Botany, Faculty of Science, Tanta University, Tanta, Egypt

The relationships among 20 samples belonging to 6 subspecies of Vicia sativa based on the variability of seed storage proteins and esterase isozyme electrophoretic patterns was discussed in relation to variation in their morphology and chromosome characters. Electrophoretic protein profiles of different accessions of the same subspecies showed identical (e.g. macrocarpa and cordata) or similar (e.g. amphicarpa) patterns, confirming the stablity of seed storage proteins within these subspecies. However, considerable variation of protein patterns were observed within accessions of both nigra and sativa subspecies, which could be correlated to different geographical origins. Esterase pattern revealed a sharp distinction for each subspecies according to the number and loci of allelic bands. The dendrogram delimited the subspecies incisa and sativa as two separate groups, while the other subspecies were grouped together in another group.

Keywords: common vetch; chromosomes; esterase; isozymes; numerical analysis; seed storage proteins
Subjects: chromosomes, Vicia subspecies; esterase isozymes, Vicia subspecies; numerical analysis, Vicia subspecies; seed storage proteins vetch subspecies; storage proteins, Vicia subspecies; vetch, common, subspecies

Published: August 1, 2000  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Haider, A.S., & El-Shanshoury, A.R. (2000). Variability of Storage Proteins and Esterase Isozymes in Vicia Sativa Subspecies. Biologia plantarum, 43(2), 205-209. doi: 10.1023/A:1002791824350
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Ball, P.W.: Vicia L.-In: Tutin, T.G., Heywood, V.H. (ed.): Flora Europe. Pp. 129-136. Cambridge University Press, Cambridge 1968.
    2. Dalling, M.J., Trunoff, P.N., Swinden, L.B.: Identification of subterranean clover (Trifolium subterraneum) cultivars on the basis of electrophoretic mobility of the seed globulin proteins.-Seed Sci. Technol. 7: 177-223, 1979.
    3. Davis, P.H. Plitmann, U.: Vicia L.-In: Davis, P.H. (ed.): Flora of Turkey. Pp. 274-325, Edinburgh University Press, Edinburgh 1970.
    4. Ferguson, J.M. Grabe, D.F.: Identification of cultivars of perennial rye grass by SDS-PAGE of seed proteins.-Crop Sci. 26: 170-176, 1986. Go to original source...
    5. Schubert, I., Rieger, R., Fuchs, J.: Alteration of basic chromosome number by fusion-fission cycle.-Genome 38: 1289-1292, 1995. Go to original source...
    6. Hamrick, J.L., Godt, M.J.W.: Allozyme diversity in plant species.-In: Brown, A.H.D., Clegg, M.T., Kahler, A.L., Weir, B.S. (ed.): Plant Population Genetics, Breeding and Genetic Resources. Pp. 43-63, Sinauer, Sutherland 1989.
    7. Hamrick, J.L., Godt, M.J.W.: Conservation genetics of endemic plant species.-In: Avise, J.L., Hamrick, J.L. (ed.): Plant Population Genetics: Case Histories from Nature. Pp. 281-304. Chapman and Hall, New York 1996. Go to original source...
    8. Hanelt, P. Mettin, D.: Cytosystematische Untersuchungen in der Artengruppe um Vicia sativa L. II.-Kulturpflanze 14: 137-161, 1966. Go to original source...
    9. Holger, K. Joachim, W.K.: Variability of isozyme pattern, chromosome number, and latex colour and components in the closely related Papaver dubium L. and P. lecoqii Lamotte in Europe.-Flora 190: 97-104, 1995. Go to original source...
    10. Holling, E. Stace, C.A.: Karyotype variation and evolution in the Vicia sativa aggregate.-New Phytol. 73: 195-208, 1974. Go to original source...
    11. Ladizinsky, G., Hymowitz, T.: Seed protein electrophoresis in taxonomic and evolutionary studies.-Theor. appl. Genet. 54: 145-151, 1979. Go to original source...
    12. Ladizinisky, G., Waines, G.: Seed protein polymorphism in Vicia sativa aggregate (Fabaceae).-Plant Syst. Evol. 41: 1-5, 1982. Go to original source...
    13. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680-685, 1970. Go to original source...
    14. Mejia, I., McDaniel, R.G.: Electrophoretic characterization of lettuce cultivars.-HortScience 21: 278-280, 1986. Go to original source...
    15. Mettin, D. Hanelt, P.: Cytosystematische Untersuchungen in der Artengruppe um Vicia sativa L. I.-Kulturpflanze 12: 163-225, 1964. Go to original source...
    16. Mettin, D. Hanelt, P.: Über Speziationvorgänge in der Gattung Vicia L.-Kulturpflanze 21: 25-54, 1973. Go to original source...
    17. Robinson, P.J. Megarrity, R.G.: Characterization of Stylosanthes introductions by using seed protein patterns.-Aust. J. agr. Res. 26: 467-479, 1975. Go to original source...
    18. Rohlf, F.J.: NTSYS-PC-Numerical Taxonomy and Multivariate Analysis. Appl. Biostatics. New York 1993.
    19. Soltis, D.S., Haufler, C.H., Darrow, D.C., Gastony, G.J.: Starch gel electrophoresis of ferns: a comparison of grinding buffers, gel and electrode buffers, and staining schedules.-Amer. Fern J. 73: 9-27, 1983. Go to original source...
    20. Watanabe, K., Yamada, T.: Studies on the interspecific hybridization of common vetch Vicia sativa L. and V. angustifolia var. segetalis Yahazuendo. I. Morphological characteristics and fertility in F1 and F2 plants.-Bull. nat. Inst. agr. Sci. 12: 1-18, 1958.
    21. Wrigley, C.W., Autran, J.C., Bushuk, W.: Identification of cereal varieties by gel electrophoresis of grain proteins.-In: Pomeranz, Y. (ed.): Advances in Cereal Science and Technology. Pp. 211-259. Amer. Assoc. Cereal Chem., St. Paul 1982.
    22. Yamamoto, K.: Studies on the hybrids among the Vicia sativa L. and its related species.-Tech. Bull. Fac. Agr. Kagawa Univ. 21: 1-104, 1966.
    23. Yamamoto, K.: On the species hybrid between Vicia sativa L. (2n=12) and Vicia angustifolia L. var. minor (2n=12).-Tech. Bull. Fac. Agr. Kagawa Univ. 19: 1-11, 1967.
    24. Yamamoto, K.: On the secondary balanced karyotypes.-Tech. Bull. Fac. Agr. Kagawa Univ. 20: 101-107, 1971.
    25. Yamamoto, K.: Hybrid plants between two races of Vicia amphicarpa having 2n=10 and 2n=14 chromosomes.-Jap. J. Breed. 42: 73-80, 1974. Go to original source...
    26. Yamamoto, K.: On the inter specific hybrid between Vicia amphicarpa and Vicia sativa.-Tech. Bull. Fac. Agr. Kagawa Univ. 28: 23-31, 1977.
    27. Zohary, M. Plitmann, U.: Chromosome polymorphism, hybridization and colonization in the Vicia sativa group (Fabaceae).-Plant Syst. Evol. 131: 143-156, 1979. Go to original source...

    Return to the content