biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 39:321-329, 1997 | DOI: 10.1023/A:1000959621891

Continuous micropropagation of juvenile larch from different species via adventitious bud formation

D. Ewald1, U. Kretzschmar1, Y. Chen2
1 Institute for Forest Tree Breeding, Federal Research Centre for Forestry and Forest Products, Waldsieversdorf, Germany
2 Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China

A continuous propagation of juvenile larch in vitro was based on adventitious bud formation and different cytokinin combinations were tested concerning their effectiveness to induce elongation of adventitious buds. Zeatin (1.5 mg dm-3) combined with kinetin (0.15 mg dm-3) was found to be the best. Development and elongation of buds was achieved on a modified LP-medium. Using this system it was possible to propagate different larchspecies (Larix decidua, L. gmelinii and L. sukaczewii f. multiramosus) continuously. Shoots were successfully rooted and transferred to the soil.

Keywords: Larix; regeneration; tissue culture

Prepublished online: April 1, 1997; Published: November 1, 1997  Show citation

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Ewald, D., Kretzschmar, U., & Chen, Y. (1997). Continuous micropropagation of juvenile larch from different species via adventitious bud formation. Biologia plantarum, 39(3), 321-329. doi: 10.1023/A:1000959621891
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