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Secondary products formation as a tool for discriminating non-Saccharomyces wine strains

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Abstract

A total of 78 strains of non-Saccharomyces yeasts were isolated: 30 strains of Kloeckera apiculata, 20 of Candida stellata, 8 of Candida valida and 20 of Zygosaccharomyces fermentati. The diversity of yeast species and strains was monitored by determining the formation of secondary products of fermentation, such as acetaldehyde, ethyl acetate and higher alcohols. Within each species, the strains were distinguishable in phenotypes through the production of different amounts of by-products. In particular, a great variability was found in C. stellata, where six different phenotypes were identified by means of the production of acetaldehyde, ethyl acetate, isobutanol and isoamyl alcohol. At different stages of the spontaneous fermentation different phenotypes of the non-Saccharomyces yeasts were represented, characterized by consistent differences in some by-products involved in the wine bouquet, such as acetaldehyde.

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References

  • Barnett JA, Payne RW & Yarrow D (1990) Yeasts: Characteristics and Identification. Cambridge University Press, Cambridge

    Google Scholar 

  • Benda I (1982) Wine and Brandy. In: Reed G (Ed) Prescott and Dunn's Industrial Microbiology, pp 293–402. Avi-Publication Company, Westport

    Google Scholar 

  • EBC Analytica Microbiologica (1981) Part II. J. Inst. Brew. 87: 303–321

    Google Scholar 

  • Fleet GH (1993) The microorganisms of winemaking — isolation, enumeration and identification. In: Fleet GH (Ed) Wine Microbiology and Biotechnology, pp 1–25, Harwood Academic Publishers

  • Fleet GH & Heard GM (1993) Yeasts — growth during fermentation. In: Fleet GH (Ed) Wine Microbiology and Biotechnology, pp 27–54, Harwood Academic Publishers

  • Frezier V & Dubourdieu D (1992) Ecology of yeast strain Saccharomyces cerevisiae during spontaneous fermentation in a Bordeaux winery. Am. J. Enol. Vitic. 43: 375–380

    Google Scholar 

  • Kreger-van Rij NJW (1984) The Yeasts: a Taxonomic Study. Elsevier Science Publishers, Amsterdam

    Google Scholar 

  • Lutstorf U & Megnet R (1968) Multiple forms of alcohol dehydrogenase in S.cerevisiae. I. Physiological control of ADH-2 and properties of ADH-2 and ADH-4. Arch. Biochem. Biophys. 126: 933–944

    Google Scholar 

  • Polsinelli M, Romano P, Suzzi G & Mortimer R (1996) Multiple strains of Saccharomyces cerevisiae on a single grape vine. Lett. Appl. Microbiol. 22

  • Romano P, Suzzi G, Comi G & Zironi R (1992) Higher alcohol and acetic acid production by apiculate wine yeasts. J. Appl. Bacteriol. 73: 126–130

    Google Scholar 

  • Romano P & Suzzi G (1993) Potential use for Zygosaccharomyces species in winemaking. J. Wine Res. 4: 87–94

    Google Scholar 

  • Schimpfessel L (1968) Présence et régulation de la synthesé de deux alcool dehydrogenases chez la levure S. cerevisiae. Biochim. Biophys. Acta 151: 317–329

    Google Scholar 

  • Schütz M & Gafner J (1993) Analysis of yeast diversity during spontaneous and induced alcoholic feermentations. J. Appl. Bacteriol. 75: 551–558

    Google Scholar 

  • Wickerham LJ (1951) Taxonomy of yeasts. I. Techniques of classification. In: U.S. Department of Agriculture, Technical Bulletin No. 1029, Washington, D.C.

    Google Scholar 

Download references

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Romano, P., Suzzi, G., Domizio, P. et al. Secondary products formation as a tool for discriminating non-Saccharomyces wine strains. Antonie Van Leeuwenhoek 71, 239–242 (1997). https://doi.org/10.1023/A:1000102006018

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  • DOI: https://doi.org/10.1023/A:1000102006018

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